Cerebroside carnosine injection with good stability as well as preparation method and application of cerebroside carnosine injection
A technology of cerebroside muscle and injection, which is applied in the field of medicine, can solve the problem of affecting the quality of drugs, quality uniformity and quality control effectiveness and safety, and increase the risk of microbial infection of rabbit muscle and its extract Rabbit muscle extract Freshness, affecting the extraction efficiency of peptide products and other issues, to achieve the effect of improving peptide extraction efficiency, improving stability and safety, and improving resource utilization
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Embodiment 1
[0054] Example 1 The present invention is used to prepare the rabbit muscle extract of cerebroside and carnosine injection
[0055] The present invention is used for the preparation of the rabbit muscle extract of cerebroside carnosine injection and comprises the following steps:
[0056] (1) Take 10kg of fresh rabbit muscle, remove the fat and connective tissue, wash it, and mince it until the volume is not higher than 1cm 3 Then add the obtained minced muscle into 10L of water for injection, mix evenly, homogenate, and then freeze the obtained muscle homogenate at not lower than -10°C for 7 days, then place it in 8 Thaw at ℃ for 36 hours, repeat the freezing and thawing treatment of the rabbit muscle homogenate twice according to the aforementioned freezing and thawing conditions, heat the obtained rabbit muscle homogenate to 60°C and keep it warm for 20 minutes, then cool it down to room temperature, Centrifuge at 4000 rpm for 20 minutes to obtain a filtrate;
[0057] (2...
Embodiment 2
[0060] Example 2 The present invention is used to prepare the rabbit muscle extract of cerebroside and carnosine injection
[0061] The present invention is used for the preparation of the rabbit muscle extract of cerebroside carnosine injection and comprises the following steps:
[0062] (1) Take 10kg of fresh rabbit muscle, grind it after pretreatment, and grind it to a volume no higher than 1cm 3 Then add 10L of water for injection to the obtained minced muscle, mix evenly, homogenate, place the obtained muscle homogenate at no lower than -10°C for 7 days, and then place it at 10°C After thawing at low temperature for 48 hours, the rabbit muscle homogenate was repeatedly frozen and thawed twice according to the aforementioned freezing and thawing conditions, and the obtained rabbit muscle homogenate was heated to 60°C, kept for 20 minutes, and then cooled to room temperature. Centrifuge at 4000 rpm for 20 minutes to obtain a primary filtrate;
[0063] (2) Adjust the pH ...
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