Method for rapidly measuring content of bongkrekic acid in food by automatic solid phase extraction-ultra performance liquid chromatography
A technology of ultra-high performance liquid phase and rice yeast acid, which is applied in the field of food safety testing, can solve the problems of short processing cycle, achieve high recovery rate, short pre-processing cycle, and reduce labor costs
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Embodiment 1-10
[0040] Embodiment 1-10: Determination of the rice yeast acid content in dry corn
[0041] 1) After crushing 10 batches of dry cornmeal purchased in the market, weigh 20 g and place them in a conical flask, add 40 mL of 2 % ammonia water and 80 % acetonitrile aqueous solution as the extraction solvent, mix well, and soak for 1 h at room temperature in the dark. Place in an ultrasonic oscillator for ultrasonic extraction for about 30 min, centrifuge at low speed, transfer the supernatant, add 40 mL of 80% acetonitrile aqueous solution of 2% ammonia to the residue, repeat the above operation once, and combine the supernatant; the supernatant is fully automatic The solid-phase extraction instrument was used for program elution and purification, using WatersOasis MAX 6cc (500mg) anion exchange column, the enrichment and elution program time is shown in Table 1, to achieve the purification and enrichment of 80 mL extraction solution;
[0042] Table 1. Fully automatic solid-phase ext...
Embodiment 11-20
[0045] Embodiment 11-20: Determination of the content of rice yeast acid in dry corn
[0046] 2) After crushing 10 batches of dried white fungus purchased in the market, weigh 20 g and place them in a conical flask, add 40 mL of 2 % ammonia water and 80 % acetonitrile aqueous solution as the extraction solvent, mix well, and soak for 1 h at room temperature in the dark. Place in an ultrasonic oscillator for ultrasonic extraction for about 30 min, centrifuge at low speed, transfer the supernatant, add 40 mL of 80% acetonitrile aqueous solution of 2% ammonia to the residue, repeat the above operation once, and combine the supernatant; the supernatant is fully automatic The solid-phase extraction instrument was used for program elution and purification, using WatersOasis MAX 6cc (500mg) anion exchange column, the enrichment and elution program time is shown in Table 1, to achieve the purification and enrichment of 80 mL extraction solution;
[0047] Table 1. Fully automatic solid...
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