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Method for simultaneously preparing shiny-leaved yellowhorn oil and shiny-leaved yellowhorn protein through aqueous enzymatic method

A technology of sorbifolia oil and water enzymatic method, which is applied to the preparation method of peptides, chemical instruments and methods, fat oil/fat production, etc., can solve the problem that the cake can only be used as animal feed, the human body and the environment are greatly affected, and the fat Quality restrictions and other issues, to achieve the effect of reduced possibility, low cost, and reduced flammability and explosion

Pending Publication Date: 2020-03-06
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] (1) The traditional pressing method produces a high temperature above 200°C during the pressing process, which greatly damages other important nutrients such as unsaturated fatty acids and vitamin K in the oil, and also leads to protein denaturation, etc. The cake is only Can be used as animal feed, etc.
[0008] (2) The organic solvent used in the extraction method for oil extraction will overflow and volatilize, which will have a great adverse effect on the human body and the environment. Problems such as solvent residues and oil quality degradation have limited its development as edible oil and health care oil.

Method used

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  • Method for simultaneously preparing shiny-leaved yellowhorn oil and shiny-leaved yellowhorn protein through aqueous enzymatic method

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Effect test

Embodiment 1

[0067] A kind of aqueous enzymatic method that the embodiment of the present invention 1 provides simultaneously prepares the method for sorbia sorbifolium oil and radix sorbifolium protein and comprises the following steps:

[0068] (1) Slurry acquisition: put the peeled X. sorbifolia nuts and water into a colloid mill at a material-to-liquid ratio of 2 g: 3 mL, crush the X. radix radix kernels to 80 mesh, and collect the slurry.

[0069] (2) Enzymolysis: Adjust the pH of the slurry ground in step 1 to 3.7, add pectinase and cellulase for enzymolysis, and perform enzymolysis in a water bath at 45°C for 2.5 hours. After the enzymatic hydrolysis reaction is completed, heat the enzymatic hydrolysis solution to 75°C to inactivate the enzyme for 15 minutes, adjust the pH of the enzymatic hydrolysis solution to 4, and then centrifuge. Centrifuge the enzymatic solution to obtain the first layer of clear oil, the second layer of emulsified layer, the third layer of waste liquid, and ...

Embodiment 2

[0076] A kind of aqueous enzymatic method that the embodiment of the present invention 2 provides simultaneously prepares the method for sorbia sorbifolium oil and radix sorbifolium protein and comprises the following steps:

[0077] (1) Slurry acquisition: put the peeled X. sorbifolia nuts and water into a colloid mill at a material-to-liquid ratio of 2 g: 5 mL, crush the X. sorbifolia nuts to 120 mesh, and collect the slurry.

[0078] (2) Enzymolysis: adjust the pH of the slurry ground in step 1 to 5.0, add pectinase and cellulase for enzymolysis, and perform enzymolysis in a water bath at 50° C. for 4 hours. After the enzymatic hydrolysis reaction is completed, heat the enzymatic hydrolysis solution to 80°C to inactivate the enzyme for 20 minutes, adjust the pH of the enzymatic hydrolysis solution to 4.5, and then centrifuge. Centrifuge the enzymatic solution to obtain the first layer of clear oil, the second layer of emulsified layer, the third layer of waste liquid, and t...

Embodiment 3

[0085] A kind of aqueous enzymatic method that the embodiment of the present invention 3 provides simultaneously prepares the method for sorbia sorbifolium oil and sorbia sorbifolium protein and comprises the following steps:

[0086] (1) Slurry acquisition: put the peeled X. sorbifolia nuts and water into a colloid mill at a material-to-liquid ratio of 2 g: 9 mL, crush the X. radix radix kernels to 160 mesh, and collect the slurry.

[0087] (2) Enzymolysis: adjust the pH of the slurry ground in step 1 to 6.0, add pectinase and cellulase for enzymolysis, and perform enzymolysis in a water bath at 65° C. for 5.5 hours. After the enzymatic hydrolysis reaction is completed, heat the enzymatic hydrolysis solution to 85°C to inactivate the enzyme for 25 minutes, adjust the pH of the enzymatic hydrolysis solution to 5, and then centrifuge. Centrifuge the enzymatic solution to obtain the first layer of clear oil, the second layer of emulsified layer, the third layer of waste liquid, ...

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Abstract

The invention belongs to the technical field of deep processing of agricultural products, and discloses a method for simultaneously preparing shiny-leaved yellowhorn oil and shiny-leaved yellowhorn protein through an aqueous enzymatic method. The method comprises the steps: obtaining slurry; carrying out enzymolysis and centrifugation; carrying out ultrasonic demulsification treatment to obtain free oil; and sequentially carrying out hydration degumming, deacidification and decoloration treatment to obtain the high-purity shiny-leaved yellowhorn oil; adding alkaline protease into a primary crude protein liquid, and carrying out alkaline extraction two times; obtaining a secondary alkali extract solution, carrying out centrifugal separation on the obtained secondary alkali extract solutionto obtain a secondary protein liquid; and adjusting the pH value, carrying out acid precipitation centrifugation, and carrying out freeze drying to obtain the shiny-leaved yellowhorn protein. The process is simple and safe, and the extracted shiny-leaved yellowhorn oil is strong in fragrance, light in color and good in quality; the prepared shiny-leaved yellowhorn protein is higher in purity, theprotein content is higher than 50%, and the shiny-leaved yellowhorn protein is suitable for industrial production. According to the method, the oil yield is increased, meanwhile, no pollution is caused, the safety is high, and a combustible solvent is not used, so that the possibility of flammability and explosiveness is reduced, the pollution is controlled, and the safety is guaranteed; the costis low.

Description

technical field [0001] The invention belongs to the technical field of intensive processing of agricultural products, and in particular relates to a method for simultaneously preparing sorbifolia oil and radix sorbifolium protein by an aqueous enzymatic method. Background technique [0002] At present, the closest prior art: sorbiflora belongs to Sapindaceae genus S. sorbifolium, also known as ramex, radix radix, etc., and is a unique precious woody tree species in my country. It is not only a nutritious and delicious fruit and a famous woody oil tree species, but also an important excellent water and soil conservation tree species that can improve the ecological environment and green the country. [0003] Xingguan fruit contains a lot of oil, the seed oil content rate is 30.4%, the seed kernel is rich in nutrition, the oil content rate is 55%-66%, and the unsaturated fatty acid content in its oil is as high as 94%, of which linoleic acid accounts for 36.9%, oleic acid It a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/04C11B1/02C11B3/00C11B3/06C11B3/10C12P21/06C07K1/30
CPCC11B1/00C11B1/04C11B1/025C11B3/001C11B3/06C11B3/10C12P21/06C07K1/30
Inventor 麻云霞李钢铁郭欣宇沈新然
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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