Method for separating and purifying T lymphocytes

A technology for separation and purification of lymphocytes, applied in the field of separation and purification of T lymphocytes, can solve problems such as limited use, unfavorable effective use of T lymphocytes, limited source of umbilical cord blood, etc., and achieve the effect of optimizing extraction conditions
CN109679903AInactive Publication Date: 2019-04-26SHENZHEN PREGENE BIOPHARMA CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHENZHEN PREGENE BIOPHARMA CO LTD
Publication Date
2019-04-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for separating and purifying T lymphocytes. The method comprises the following four steps: 1) separating plasma; 2) separating lymphocytes; 3) cleaning lymphocytes; 4)purifying lymphocytes. According to the technical scheme of the invention, extraction conditions of T lymphocytes are optimized and effective utilization of T lymphocytes is benefited.
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Description

technical field

[0001] The invention relates to the technical field of cell extraction and separation, in particular to a method for separating and purifying T lymphocytes. Background technique

[0002] T cells are one of the two major lymphocytes in the human body and play an extremely important role in specific cellular immunity. Mature T cells settle in the thymus-dependent area of ​​the peripheral immune organs of the human body, and can circulate through lymphatic vessels, peripheral blood, and interstitial fluid to exert cellular immunity and immune regulation functions. Because T cells have extremely strong specificity and good killing effect in cellular immunity, T cells have broad application prospects in basic immunology research and in the biopharmaceutical industry related to immunotherapy. Usually, in order to obtain a better extraction effect, T cells are separated and extracted by collecting umbilical cord blood. Due to the limited source of umbilical cord b...

Claims

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