The invention refers to microfluidic separation of plasma for colormetric assay. A device includes a plasma separation membrane (110), a capillary channel (115) positioned adjacent the plasma separation membrane to receive plasma from blood placed on the plasma separation membrane, at least one cuvette (125, 245, 250, 255, 260, 332, 334, 336, 338, 342, 344, 346, 348, 420, 425, 430, 435.540, 545, 550, 555, 650, 652, 656) coupled to the capillary channel, a gas permeable membrane (150, 270, 350, 355, 622), and a distribution channel (120, 230, 235, 240, 325, 330, 525, 530, 545, 630) coupled to the capillary channel to provide plasma to the cuvette, wherein the cuvette is configured to hold an amount of plasma with reagent suitable for colorimetric assay by a tester to hold the device. Variations include the use of a quantiation channel (612) to provide a selected amount of plasma and a mixing channel (616) to mix plasma with a diluent.