Whole blood centrifugal separation chip and preparation method thereof

A centrifugal separation and chip technology, applied in the field of micro-electromechanical systems, can solve the problems of large consumption of sample biochemical reagents, restricting the development of biochemical analysis, and difficulty in realizing automation, etc., and achieve the effect of compact structure, reduced total area, and short time consumption

Inactive Publication Date: 2011-09-07
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as an indispensable part of the system, pretreatment technologies such as sample separation have developed relatively slowly, and have become the bottleneck in the entire analysis process, which restricts the development of biochemical analysis.
Existing sample pretreatment technologies are often implemented off-chip, and most of them have disadvantages such as time-consuming, labor-intensive, difficult to automate, poor precision, and large consumption of samples and other biochemical reagents, and are often the main cause of measurement errors
The traditional sample separation technology can no longer meet the needs of the development of μTAS (Micro total analysis system), it is necessary to develop a new micro separation technology

Method used

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  • Whole blood centrifugal separation chip and preparation method thereof
  • Whole blood centrifugal separation chip and preparation method thereof
  • Whole blood centrifugal separation chip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1: Process a centrifugal separator on a silicon wafer

[0065] The structure of this embodiment is shown in Figure 1, and the process flow is shown in figure 2 .

[0066] 1) The process flow of the silicon wafer structure of the chip:

[0067] (a) processing and cleaning the silicon wafer 8;

[0068] (b) Throwing the photoresist 9 on the front side of the silicon wafer, pre-baking, photolithography, developing, and post-baking;

[0069] (c) In-depth etching (ICP) of about 100 μm of silicon on the front side of the silicon wafer to form a microfluidic channel 4, a micro column fence 5, 6, an inlet 2, and three outlets 3;

[0070] 2) Chip cover polymer process:

[0071] (d) process and clean the silicon wafer 8, and apply a release agent 10 on its surface;

[0072] (e) Pour the bubble-free silicon rubber photoresist (PDMS) 11 into the petri dish, let it stand for flattening, and then bake it in an oven at 80°C for about 1 hour;

[0073] (f) Cut the cured ...

Embodiment 2

[0075] Example 2: Fabrication of centrifugal separators from polymers

[0076] The structure of the present embodiment is shown in Fig. 1, and the process flow is shown in the appended image 3 .

[0077] 1) Process flow of polymer structure of separator:

[0078] (a) processing and cleaning the silicon wafer 8;

[0079] (b) Throwing the photoresist 9 on the front side of the silicon wafer, pre-baking, photolithography, developing, and post-baking;

[0080] (c) In-depth etching (ICP) of about 100 μm of silicon on the front side of the silicon wafer to form microfluidic channels 4, micro column fences, sample inlets and sample outlets;

[0081] (d) Process and clean the silicon structure mold 13, and apply a release agent 10 on its surface, pour the bubble-free PDMS 11 on the silicon structure mold 13, and let it stand for flattening, then bake in an oven at 80°C Bake for about 1 hour;

[0082] (e) peel off the cured PDMS from the silicon mold 13, and cut each unit;

[00...

Embodiment 3

[0088] Embodiment 3: the centrifugal separator of sandwich structure

[0089] The technological process of this embodiment sees Figure 4 .

[0090] (a) processing and cleaning the silicon wafer 8;

[0091] (b) Throwing the photoresist 9 on the front side of the silicon wafer, pre-baking, photolithography, developing, and post-baking;

[0092] (c) In-depth etching (ICP) of about 50 μm of silicon on the front side of the silicon wafer to form a microfluidic channel 4, a micro column fence, an inlet 2, and an outlet 3 (similar in structure to method 1);

[0093] (d) the front side of the silicon wafer is anodically bonded to the glass 15;

[0094] (e) Thinning the silicon wafer on the back side of the silicon glass sheet after bonding, the method of dry method, wet method or CMP can be adopted;

[0095] (f) Photoresist removal on the back of the silicon wafer, pre-baking, photolithography, development, and post-baking;

[0096] (g) Deeply etch the inlet and outlet sample po...

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Abstract

The invention discloses a whole blood centrifugal separation chip and a preparation method thereof and belongs to the field of micro-electromechanical system. A vortex-type microfluid passage is arranged on the chip; a sample inlet is arranged in the passing center of the microfluid passage; at least one row of micro upright post rails are arranged in the microfluid passage; the microfluid passage is divided into two or more flow passages by the micro upright post rails; and the flow passages are connected with different sample outlets. By pouring the whole-blood sample into the centrifugal separation chip through a micro pump, cells and blood plasma are separated by gaps between the micro upright post rails; the structure of the chip is compact, so the total area of the chip is reduced; the separating efficiency is enhanced; and the separating process of the chip costs a short time.

Description

technical field [0001] The invention belongs to the field of micro-electromechanical systems, and in particular relates to a whole blood centrifugal separation chip and a preparation method of the chip for separation, detection and analysis of biological samples. Background technique [0002] The 21st century is an era of interdisciplinary development, especially the development of biochips and biochemical detection technology. Sensing technology is an important means of information acquisition, and using sensing technology to obtain biological sample information is an important content of the development of biological detection technology. [0003] BioMEMS (biological micro-electro-mechanical systems) technology, which combines biotechnology and micro-electro-mechanical systems (MEMS) technology, can realize continuous, integrated, miniature , so as to obtain the so-called micro-full analysis system. The system includes sample injection, separation, reaction and detection...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48
Inventor 李志宏耿照新王玮鞠衍睿
Owner PEKING UNIV
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