35 results about "Micro column" patented technology

The present application belongs to the technical field of microfluidic chip, and particularly relates to a two-section cell sample sorting microfluidic chip and a sorting method thereof. A rectangular array composed of multiple columns of micro-pillars is arranged in the main flow channel of the chip, and the column offset angle between adjacent two columns of micro-pillars periodically changes along the sample flow direction. In one change cycle, each column of micro-pillars is first deflected by a preset gradient angle column by column along a first direction until the cumulative deflection angle reaches a limit angle, and then is deflected along a second opposite direction until the limit angle is reached, and the cycle is repeated. By arranging the micro-pillar array with periodically changing column offset angle, a dynamic flow field is created in the main flow channel, and high stiffness fibrous particles are actively induced to rotate and change their poses, and their effective hydrodynamic size is dynamically amplified, so that accurate sorting of fibrous biological particles based on morphology and length is realized, and the risk of sample damage and clogging is reduced, which is suitable for the rapid detection needs of clinical complex samples.

This application provides an in-situ micropillar compression experiment alignment method, relating to the field of micro / nano mechanical testing instrument technology. The method includes: acquiring a sample; providing a mechanically self-locking alignment device, placing the sample and an in-situ indenter on the mechanically self-locking alignment device to perform macroscopic positioning of the sample, and transferring the sample to the in-situ indenter; removing the in-situ indenter from the mechanically self-locking alignment device and placing it under an electron microscope; controlling a probe to sequentially contact two marks, acquiring coordinate data of the two marks respectively; obtaining the positional deviation of the micropillar relative to the marks in the probe's motion coordinate system based on the coordinate data; controlling the probe to move directly above one of the marks based on the coordinate data; and controlling the probe to move from directly above the mark to directly above the micropillar based on the positional deviation. This application can solve the significant shortcomings of existing sample mounting and alignment technologies in terms of positioning accuracy, operational safety, and engineering reliability.

The invention provides a micro-column center determination method and device using white light interference scanning and a visual identification algorithm. The method comprises the following steps: acquiring a white light interference scanning image sequence of a micro-cylinder sample from bottom to top; preprocessing the image sequence to obtain a two-dimensional grayscale image; segmenting a micro-column region by using a visual identification algorithm; determining a threshold value based on a micro-column design area, and screening a target Blob region; calculating the Blob centroids of all pixel points in the target Blob area, carrying out cross validation on the Blob centroids and a micro-column contour fitting center, and determining XY centers (X0, Y0); screening part of pixel points close to the highest point in the target Blob area, and calculating the mean value Z0 of Z values of the pixel points; obtaining a three-dimensional center (X0, Y0, Z0); and repeatedly searching the three-dimensional center, and verifying repeatability and accuracy. The method can be applied to micro-column center positioning requirements of engineering cases, and technical support is provided for reliable operation of coating related devices under extreme working conditions.

The invention provides a film curing stress testing device and method, and belongs to the technical field of film mechanical property testing. The film curing stress testing device comprises a substrate, a rigid base, an optical camera module, a stress calculation module and a plurality of flexible micro-columns, the top surface of the substrate is used for coating a to-be-tested film sample, and the bottom surface of the substrate is adhered to the plurality of flexible micro-columns; the plurality of flexible microcolumns are arranged on the rigid base and are distributed on the edge of the bottom surface of the substrate at intervals, and the plurality of flexible microcolumns are used for being uniformly stressed and deforming when the substrate deforms in the curing process of the to-be-detected film sample; the optical camera module is arranged on the side surface of the substrate and is used for collecting the displacement variation of each mark point; and the stress calculation module is used for determining the curing stress of the to-be-measured film sample according to the displacement variation of each mark point. According to the invention, the curing stress change rule of the to-be-tested film sample can be obtained in real time under the condition that the test cost is relatively low and the test precision is relatively high.

The present application relates to a test reagent for determining blood group antigens. The test reagent comprises: an antibody or antigen-binding fragment thereof against blood group antigens, sodium salt, potassium dihydrogen phosphate, bovine serum albumin, glycine, sugar, optionally a polymerization promoter, a preservative. After the test reagent of the present application is mixed with red blood cells, a positive result can form visible agglutination in an anti-human globulin test card or a blood group reverse typing test card based on micro-column gel method.

The application discloses a kind of V-shaped slope stability calculation method and system, it is related to strip mining technical field.Based on three-dimensional numerical simulation technology, determine its failure mode first determine the spatial form equation of potential sliding body, then the sliding body is divided into regional division micro column, through constitutive relation and static equilibrium condition, the stress state of micro column is solved, the force of each row micro column is superposed along the direction of main sliding surface, and the two-dimensional equivalent shear strength parameters with three-dimensional effect on each row of main sliding surface micro column are obtained using equivalent idea, then the three-dimensional V-shaped slope stability coefficient is derived using rigid body limit equilibrium method.The application avoids the problems of high calculation cost, difficult parameter determination and complex calculation process in the traditional three-dimensional slope stability calculation method, solves the three-dimensional stability problem of this special form slope, provides more reliable theory and technology for the design, mining, prevention and management of slope, and has important practical significance for ensuring mining safety and efficient production.

The invention discloses a mixed wettability nanowire modified three-stage enhanced boiling heat transfer structure and a preparation method thereof, and belongs to the technical field of micro-nano processing and enhanced phase change heat transfer. According to the structure, two kinds of functional micro-column arrays, namely a micro-cylinder array modified by a super-hydrophilic nanowire and a micro-square column array modified by a super-hydrophobic nanowire, are integrated on a substrate. An efficient liquid supplementing channel is constructed through the strong capillary action, and local dryness is effectively inhibited; the latter is used as a low-superheat-degree nucleation site, rapid generation and separation of bubbles are promoted, bubble coalescence can be effectively inhibited through the millimeter-level spacing, and formation of a steam film is delayed. The two types of micro-columns are orderly distributed in space, and a bubble separation path and a liquid backflow channel are collaboratively optimized. The structure integrates the multi-scale enhanced boiling heat transfer advantages of the millimeter-scale hydrophobic nucleation interval, the micron-scale cylinder and the nano-scale linear structure, the heat transfer coefficient and the critical heat flux are improved, and the structure has important application potential in the high-heat flux heat dissipation fields of high-power electronic devices, aerospace, new energy equipment and the like.

The invention discloses a circulating tumor cell physicochemical screen and application thereof. The circulating tumor cell physicochemical screen comprises a body, a reaction tank is arranged in the body, at least two liquid inlets are formed in one end of the reaction tank, a liquid outlet is formed in the other end of the reaction tank, a plurality of reaction areas are arranged between the liquid inlets and the liquid outlet, and a plurality of micro-columns are arranged in each reaction area; along the direction from the liquid inlet to the liquid outlet, the distance between the micro-columns in each reaction area is gradually reduced. The circulating tumor cells are sorted in a mode of combining physical blocking and chemical adsorption, the distance between the microcolumns in each reaction area is gradually reduced, screening of the CTC cells is facilitated, further observation and judgment are also facilitated, compatibility to various coloring agents is good, and the application range is wide.

The present application relates to the field of material micro-experiment, and aims to provide a method for ensuring that a micro-column is a single crystal structure and determining the grain orientation of the micro-column in the process of preparing a single crystal micro-column in situ micro-experiment of polycrystalline material, so as to improve the accuracy and reliability of the in situ micro-experiment of the material. The technical scheme is: a method for preparing a single crystal micro-column with a specific crystal orientation in polycrystalline material based on an SEM-FIB-EBSD integrated system, comprising: polishing and fixing a polycrystalline material sample on a sample table; positioning a target area by an SEM probe, highlighting the grain boundaries of multiple crystals in the target area by irradiating the target area with a large beam of FIB; characterizing the irradiated target area by an EBSD probe, selecting a single crystal region corresponding to a crystal orientation of interest according to a grain orientation distribution map after characterization; based on the grain boundary shape of the single crystal region of interest in EBSD, locating the single crystal region by comparing and finding the same grain boundary shape under the FIB probe, and then precisely irradiating and preparing a single crystal micro-column by gradient adjusting the FIB beam.

The application provides a multi-component quantum dot heterostructure and a preparation method and application thereof. The preparation method comprises the following steps: dispersing at least two components of colloidal quantum dots in an alkane solvent to obtain a precursor solution; placing the precursor solution on the surface of a silicon-based micro-column template, then covering a base on the top of the silicon-based micro-column template, applying pressure, so that the precursor solution forms a capillary liquid bridge between the top of the silicon-based micro-column template and the base; and performing one-way evaporation treatment on the capillary liquid bridge structure to form a multi-component quantum dot heterostructure with size-oriented phase separation; the particle size of colloidal quantum dots of any component is different from the particle size of colloidal quantum dots of other components. The application provides a one-step method for preparing a multi-component quantum dot heterostructure, realizes the size-oriented phase separation and ordered assembly of the multi-component quantum dots, and has the advantages of simple process, large-scale microprocessing, good photoelectric performance and integration, and good application prospect in optoelectronic devices.

The application relates to the technical field of medical instruments, and provides an analysis information display method and device, electronic equipment and a readable storage medium, which comprise the following steps: acquiring a micro-column gel image from a sample analyzer; receiving a splicing test instruction, and sending the splicing test instruction to the sample analyzer; acquiring sample column images of test samples in the plurality of gel columns from the sample analyzer; inserting the sample column images acquired from the sample analyzer into two or more adjacent micro-column gel images; and converting the two or more adjacent micro-column gel images into two or more gel card images. The application not only realizes real display of detection results of a gel card, helps a user to accurately read results, shows agglutination strength of each gel column and real effects of tests, and further avoids waste of gel columns.

The invention belongs to the technical field of medical ultrasonic instruments, and particularly relates to a medical coupling agent bubble removal extrusion head, a storage bottle and ultrasonic detection equipment. According to the extrusion head, through the collaborative design of the shell and the double-layer defoaming membrane assembly, efficient gas-liquid separation in the medical coupling agent extrusion process is achieved, and interference of coupling agent bubbles on ultrasonic detection is eliminated from the source. The defoaming film with the polymer thermal shrinkage film as the base material is provided with a micro-column array, a directional air sliding channel is formed through cooperation of a micro-nano gap net and back exhaust holes, full-area super-hydrophobic modification enables a coupling agent to maintain a Karse state, air permeability and liquid impermeability are achieved, and the problem of leakage of a traditional structure is effectively solved. The two bubble removing films are oppositely arranged to form a micron-sized coupling agent flow channel, the micron-sized coupling agent flow channel adapts to the flow characteristic of a high-viscosity coupling agent, accurate capturing and discharging of bubbles are achieved through viscous resistance difference, no extra power source is needed, biological safety is guaranteed through pure physical bubble removing, micron-sized bubbles in the coupling agent are efficiently removed, and ultrasonic imaging artifacts are avoided.

The invention discloses a pressure and temperature coupling monitoring method and system in radioactive column separation, and belongs to the field of microfluidic monitoring, and the method comprises the following steps: injecting pressure oscillation with a predetermined frequency into a microcolumn; detecting deformation signals generated at the inlet and the outlet of the micro-column due to internal pressure change; extracting an inlet response amplitude and an outlet response amplitude corresponding to the pressure oscillation frequency and a phase difference between an inlet response and an outlet response from the deformation signal; calculating the propagation time of the pressure oscillation from the inlet to the outlet based on the phase difference; and according to the propagation time and inherent structure parameters of the micro-column. The pressure oscillation generating device adopting non-intrusive sensing design and micro-scale adaptation can be realized, the problems of blockage of a micro-column flow channel and flow field interference caused by intrusive arrangement of a traditional macroscopic sensor are avoided, meanwhile, through calculation of parameters such as phase difference and equivalent sound velocity, measurement distortion caused by micro-scale temperature-pressure coupling is effectively eliminated, and the measurement precision is improved. And the accuracy of monitoring the internal pressure of the micro-column is improved.

The invention relates to the technical field of separation and detection, in particular to a method for synchronously analyzing long-chain PAEs and short-chain PAEs based on liquid-phase sodium extraction, which comprises the following steps: preparing a carbon nanofiber-carbon fiber composite confinement material; after ultrasonic treatment, filling a confinement channel with the mixture to obtain a micro-column confinement extraction device; pre-wetting the micro-column confinement extraction device by adopting a confinement solvent to obtain the micro-column confinement extraction device with a solvent confinement system; the method comprises the following steps: enabling a sample solution containing a phthalate compound to pass through a micro-column confinement extraction device, then desorbing by adopting an elution solvent through the micro-column confinement extraction device, and collecting an eluent; and carrying out gas chromatography-mass spectrometry analysis on the collected eluent, and qualitatively and / or quantitatively detecting the phthalate compounds in the eluent. The method can cover sample pretreatment and analysis detection of various PAEs at the same time, has high sensitivity and high selectivity, and is low in organic solvent consumption and high in analysis speed.

The invention provides a fluid characterization and perturbation system, method and application. The micro-column array comprises a base and a micro-column array arranged on the base, the micro-column array is composed of one or more micro-columns, the micro-columns can deform under the action of fluid and / or magnetic force, and different micro-columns can be different in length and hardness; the bottom end and the side face are connected to the base, the top end is far away from the base and opposite to the bottom end, the micro-column is provided with a light reflecting layer, and the light reflecting layer can be arranged at any one or more positions of the top end, the side face and the interior of the micro-column. The system and the method have multi-mode characteristics, can comprehensively measure the viscosity, the density and the type of the fluid as well as the fluid direction, the pressure intensity, the shearing force and other data at a specific position, and provide a comprehensive solution for monitoring and analyzing the fluid state.

The application discloses a kind of open-pit mine horizontal mining inner discharge pressure slope under the action of three-dimensional stability calculation method of side slope, comprising: S1, according to the form of the side slope of the mining area in the research area, weak layer of sliding body and potential sliding surface form, using three-dimensional numerical simulation technology, potential sliding surface spatial form equation is established;S2, based on spatial form equation, the side slope is divided into several i rows, j column micro column, and the stress analysis is carried out to each micro column, respectively calculate the anti-sliding force and the sliding force of each micro column in each region;S3, the force of each row micro column is superimposed along the main sliding line direction, and the total anti-sliding force and the total sliding force on the main sliding line are obtained;S4, three-dimensional slope stability problem is converted into two-dimensional problem solution, and the stability coefficient is calculated using unbalanced thrust method, and the application can provide important theoretical support and technical guidance for safe and efficient recovery of deep slope coal resources.

The invention relates to a microcolumn structure, a phase change heat sink and an electronic device. The micro-column structure comprises a narrow-diameter part which is used for being thermally coupled to a part to be cooled; the wide-diameter part is fixed to the narrow-diameter part and located on the side away from the to-be-cooled part, and the peripheral contour area of the cross section of the wide-diameter part is larger than that of the cross section of the narrow-diameter part in the direction away from the to-be-cooled part. The micro-column structure can improve the capacity of transferring heat to the phase change heat absorption working medium.

The invention discloses an integrated micro-fluidic metasurface chip for real-time detection of exosome (POCT). The chip is formed by bonding a micro-fluidic structure layer and an optical sensing substrate layer, and sequentially comprises a sample / buffer solution converging region, a gradient film pretreatment region, a snake-shaped mixing region, a micro-column enrichment region and a multi-channel super-surface detection region in the flow direction. The coarse filtration membrane and the fine filtration membrane realize graded removal of cell debris and platelets, and are matched with a back-flushing structure to reduce blockage of the filter membranes; the microcolumn array is modified by a general exosome antibody and is used for efficient immune enrichment and mild washing or elution of exosome; the multi-channel metasurface array is used for respectively fixing different disease-related probes and carrying out high-sensitivity parallel optical detection on multiple markers of exosomes. According to the present invention, the single-chip integration of exosome pretreatment, enrichment and detection is achieved, the sample loss is reduced, the detection sensitivity and the flux are improved, and the method is suitable for the POCT application scenarios such as tumor early screening. Figure 1 is a structural diagram of the integrated micro-fluidic metasurface sensing chip provided by the invention.

The application provides an enhanced micro-mixing device for nucleic acid detection and a control method thereof. The device is sequentially connected from top to bottom with an upper plate, a reinforced mixing channel, a medium layer, an electrode layer and a lower plate. The reinforced mixing channel comprises a first groove for connecting a liquid storage area on the electrode layer to a mixing reaction area, a second groove for connecting a recovery area on the electrode layer to the mixing reaction area, and a micro-column array arranged at the intersection of each groove. The electrode layer is divided into a liquid storage area, a droplet generation area, a mixing reaction area and a recovery area. The electrode layer comprises a plurality of first electrodes for storing droplets, a plurality of second electrodes for pinching off droplets, a plurality of third electrodes for mixing droplets to generate reactions, a plurality of fourth electrodes for recovering droplets after reactions, and a plurality of fifth electrodes for transmitting droplets between areas. The device drives droplet generation, transmission and mixing based on the EWOD technology, and enhances flow field disturbance through the micro-column array to improve mixing efficiency.

The invention discloses a miRNA (micro Ribonucleic Acid) quantum detection method based on plasmon enhancement. The method comprises the following steps: modifying gold nanocubes on the surface of a fluorescent nano-diamond to generate the nano-diamond with a local surface plasmon resonance enhancement effect; the preparation method comprises the following steps: preparing a plasmon enhanced nano-diamond probe and a silicon dioxide microsphere probe, and hybridizing target miRNA to form a sandwich structure compound; injecting the compound into a micro-fluidic chip with a micro-column array structure, so as to entrap and enrich the compound with the sandwich structure, and filtering to remove the uncombined plasmon enhanced nano-diamond probe; the compound is subjected to fluorescence signal detection, and quantum signal detection is combined, so that qualitative or quantitative analysis of the target miRNA is realized. The background of the free probe is reduced through microfluidic filtration, the detection repeatability is improved, the nanodiamond reading signal intensity is improved by combining plasmon enhancement, and the method has the advantages of being high in sensitivity, high in specificity, convenient to operate, suitable for trace sample detection and the like.

The invention relates to the technical field of oil-water separation, in particular to a preparation method of a composite Janus thermal shrinkage film and an oil-water emulsion separation system. The oil-water emulsion separation system comprises a composite Janus thermal shrinkage film, a separation assembly and two collection assemblies. A super-hydrophobic membrane with a micro-column and micro-pore composite array and a super-hydrophilic membrane with a micro-column and micro-pore composite array are arranged on the two sides of a channel of a separation assembly respectively, and micro-columns are arranged oppositely. According to the oil-water emulsion separation system in the scheme, the oil-water emulsion can be demulsified through the micro-column and micro-pore composite array, so that separation of oil and water in the oil-water emulsion is accelerated, and the oil-water separation rate is further accelerated through the super-hydrophilic membrane and the super-hydrophobic membrane on the two sides of the channel; the constructed micro-column micropore composite array can achieve efficient oil-water separation through the synergistic effect of macroscopic flow guide demulsification and microcosmic precise screening, and then the separation effect of the whole oil-water emulsion separation system is improved.

The invention discloses a method for predicting the liquid absorption performance of a micro-column array capillary material based on image recognition. The method comprises the following steps: determining the porosity of the micro-column array capillary material; determining the total coverage area fraction of all micro-columns in the micro-column array capillary material and the average radius of the included micro-columns; according to the porosity, the total coverage area fraction and the average radius of the micro-column array capillary material, predicting the liquid absorption performance parameters of the micro-column array capillary material; and drawing a function diagram for predicting the liquid absorption performance parameters of the micro-column array capillary material according to a prediction formula of the liquid absorption performance parameters based on the porosity, the total coverage area fraction and the average radius of the micro-column array capillary material. According to the method, the liquid absorption performance parameters of the micro-column array capillary material can be accurately predicted under the condition that a liquid absorption experiment does not need to be carried out, structural parameters can be reversely deduced and designed according to the target liquid absorption performance parameters, and the structural design time of the micro-column array capillary material and the performance screening experiment cost can be greatly saved.

The invention belongs to the field of cell sample detection, and provides a micro-fluidic chip for separating CTC based on size and aptamer. The functional layer is provided with a sample outlet and a sample inlet, the cell substrates are arranged on the upper surface and the lower surface of the functional layer, the sample outlet and the sample inlet of the functional layer are communicated through a separation flow channel, and the separation flow channel comprises at least one micro-column array capable of carrying EpCAM aptamers. The micro-column array is formed by bypassing at least two micro-column structures by the capturing channel, one end of the micro-column array is connected with the sample inlet, and the other end of the micro-column array is provided with a gradient interception channel connected with the sample outlet. According to the micro-fluidic chip, an EpCAM aptamer is adopted to modify a micro-column array, so that the collision frequency of CTCs and the chip is increased, and the collision probability of circulating tumor cells and the aptamer on the surface of a micro-column is increased; and secondly, the high-specificity recognition capability of the aptamer on tumor cells is utilized, so that the separation accuracy and specificity are improved.

The invention belongs to the technical field of micro-nano processing and functional material preparation, and particularly relates to a reticular interconnected microstructure and a preparation method and application thereof. The net-shaped interconnected microstructure comprises a substrate and a micro-column array constructed on the surface of the substrate; each micro-column in the micro-column array sequentially comprises a polymer micro-column, a mixed interface layer and a UV glue micro-column from the surface of the substrate to the top; the micro-columns and the mixed interface layers of the adjacent micro-columns are mutually crosslinked to form a net-shaped interconnection structure; the polymer is PMMA (polymethyl methacrylate) or PS (polystyrene); and the UV adhesive is an acrylic ester UV adhesive containing a silicon group. According to the method, the inherent cognition that in a traditional micro-nano imprinting technology, polymer interface mutual dissolution is regarded as a residual defect is broken through, a pressure-induced interpenetrating network structure serves as a precursor of a net-shaped interconnection structure, one-step conversion is achieved through the etching rate difference, and the industrial problem that it is difficult to integrally prepare the interconnection structure and a main body structure is solved.

Micro gas chromatographic devices are provided having a microfluidic separation column and a plurality of capillaries where the capillaries have been independently configured in terms of the capillary length, capillary width, the packing density and packing geometry of the capillary using one or more micro pillars, the tortuosity of the capillary path, and the presence and identity of the stationary phase for use in micro gas chromatographic separation of complex mixtures of compounds. Through the plurality of capillaries, the devices are capable of discriminating between complex samples even in instances where complete separation of the components is not possible. Methods of fabrication and methods of use of the devices are also provided. The devices can be readily fabricated using known techniques. The devices can be used for the analysis of complex mixtures of compounds containing tens or hundreds of compounds in which just a few differ in presence or concentration.

The invention discloses a gas sample concentration chip and a preparation method thereof. The gas sample concentration chip comprises a silicon base, a cover plate, a cylindrical micro-column array, a microfluidic dam, a gas flow micro-channel, a filler injection port and a gas flow inlet / outlet, a flow channel is processed at the upper end of the silicon base through an etching process, and the silicon base and the cover plate are bonded and sealed to form a flow channel cavity; the cylindrical micro-column array is arranged in the flow channel; the microfluidic retaining dams are arranged on the two sides of the flow channel respectively; a filling injection port communicated with the runner cavity is formed in the area, corresponding to the cylindrical micro-column array, of the cover plate; an airflow micro-channel is formed in the outer side area of the microfluidic dam, and the airflow inlet / outlet is formed in the cover plate. The device has the advantages of being small in dead volume, short in airflow path, small in thermal capacity, fast in thermal response, uniform in flow field, high in stability, high in temperature monitoring accuracy, good in sealing performance and high in reliability.

The invention discloses a detection system for tracking pollutants in real time by using blue-green algae fluorescence signals, and relates to the technical field of environment monitoring. Comprising a PDMS micro-fluidic chip main body which is prepared from a polydimethylsiloxane material and comprises an inlet channel detection chamber and an outlet channel, and the top of the PDMS micro-fluidic chip main body is covered with a high-transmittance glass cover plate; the gradient density micro-column array is embedded into the bottom of the detection chamber, Young modulus among five micro-columns increases linearly, and a groove is formed in the top of the gradient density micro-column array; the cyanobacteria cell layer is anchored in the groove through Van der Waals force; the optical detection module comprises a pulse light source fluorescence sensor and an ARM microcontroller; the self-adaptive capture regulation and control unit is connected with the water sample pump microcontroller, and is used for self-adaptively capturing cyanobacteria cells and synchronously adjusting the light source and the water flow period. The method solves the contradiction between fluid disturbance and cell fixation, greatly improves the stability of fluorescence signals, retains the activity of blue-green algae without an adhesive, prolongs the continuous monitoring time, adapts to different flow velocities and environments, is accurate and reliable in detection, and meets the real-time tracking requirements of pollutants.

The application discloses a resonance chip, which comprises a glass substrate, a resonance base, a resonance cover plate and a piezoelectric sensor. The resonance base is arranged on the top surface of the glass substrate, and a microchannel is arranged on the resonance base. One end of the microchannel is provided with a channel inlet, and the other end is provided with a channel outlet. A plurality of micro columns are arranged in the microchannel. The resonance cover plate is arranged on the top surface of the resonance base. The resonance cover plate is provided with a sample inlet corresponding to the channel inlet of the microchannel, and a sample outlet corresponding to the channel outlet of the microchannel. The piezoelectric sensor is arranged on the top surface of the glass substrate. The application further discloses a preparation method of the resonance chip and application thereof. The piezoelectric sensor drives the micro column array to generate controllable acoustic flow shear force to non-contact physically stimulate cells. In the case of maintaining the activity of the cells, the application can realize efficient and safe preparation of extracellular vesicles.

The invention provides an electroplastic in-situ testing method and an electroplastic in-situ testing device, and relates to the technical field of in-situ tests.The method comprises the steps that a metal sample is prepared, and the metal sample comprises a micro-column structure; electrically connecting one pole of a pulse power supply with the metal sample, and electrically connecting the other pole with the pressure head; pressing a pressure head on the micro-column structure, regulating and controlling the output current of the pulse power supply, and regulating and controlling the stress of the pressure head on the micro-column structure, so that the micro-column structure generates plastic deformation; and recording the plastic deformation process of the micro-column structure by using a scanning electron microscope to obtain a corresponding relation among output current, stress and deformation. According to the device and the method, metal electroplasticity in-situ testing can be realized, and the dynamic deformation process and structural evolution of a micro-nano-scale micro-column structure can be clearly and continuously captured and recorded.

The application discloses a pressure and temperature coupling monitoring method and system in radioactive column separation, and belongs to the field of microfluidic monitoring, and the method comprises the following steps: injecting a pressure oscillation with a predetermined frequency into a microcolumn; detecting deformation signals generated at the inlet and outlet of the microcolumn due to internal pressure changes; extracting an inlet response amplitude, an outlet response amplitude and a phase difference between the inlet response and the outlet response corresponding to the pressure oscillation frequency from the deformation signals; calculating a propagation time of the pressure oscillation from the inlet to the outlet based on the phase difference; and determining the propagation time and inherent structural parameters of the microcolumn. The pressure oscillation generating device can be designed by using a non-invasive sensing method and is adapted to the microscale, thereby avoiding the problems of blockage and flow field interference of the microcolumn flow channel caused by the invasive arrangement of traditional macroscopic sensors, and effectively eliminating the measurement distortion caused by the temperature and pressure coupling at the microscale through the calculation of parameters such as the phase difference and the equivalent sound speed, so that the accuracy of the internal pressure monitoring of the microcolumn is improved.