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Method for extracting gastrodin by biological enzyme method

A technology of enzymatic extraction and gastrodin, which is applied in the preparation of sugar derivatives, sugar derivatives, and sugar derivatives, etc., can solve the problem of inability to extract gastrodin to the maximum extent, consumption of energy and solvent, and low extraction yield. and other problems, to achieve the effect of improving the technical level, increasing the rate of paste production, and improving the extraction rate

Inactive Publication Date: 2011-04-27
ENZYME ENG INST SHAANXI PROVINCE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two extraction processes require high equipment, consume more energy and solvents, have low processing capacity, and cause serious pollution. More importantly, the extraction yield is low, and the gastrodin contained in Gastrodia elata cannot be extracted to the maximum extent.
Therefore, the extraction process of gastrodin with high efficacy, high extraction yield and low pollution is highly expected, and there is no report on the method of extracting gastrodin by enzymatic hydrolysis technology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0014] Example 1. (gastrodia elata is purchased from Yangxian Market, Shaanxi Province)

[0015] Weigh 4.0g of cell wall-breaking enzyme, 3.0g of amylase, 3.0g of pectinase, and 1.0g of protease and mix them for later use; crush the dried Gastrodia elata through a 20-mesh sieve, weigh 200g, and dissolve in 500mL at a concentration of 0.18 mol / L, acetic acid-sodium acetate buffer solution with a pH of 4.8, then add the compound enzyme powder, and stir well; enzymolyze at 50°C for 2 hours, then add 1500 mL of alcohol, and stir thoroughly for 10 minutes; use a pore size of 8 Filter the residue with a micron filter, extract the residue twice with alcohol, combine the extracts and concentrate them into an extract by rotary steaming (temperature at 56°C, speed at 40r / min), and recover ethanol during the concentration process , and finally dry the extract at 80°C to obtain the gastrodin extract.

[0016] The yield of gastrodin determined by HPLC was 0.24%, while the yield of gastrod...

example 2

[0017] Example 2. (Gastrodia elata is purchased from the market in Lantian County, Shaanxi Province)

[0018] Weigh 3.0g of cell wall-breaking enzyme, 2.0g of amylase, 2.0g of pectinase, and 1.0g of protease and mix them for later use; crush the naturally dried Gastrodia elata through a 20-mesh sieve, weigh 120g, and dissolve in 300mL at a concentration of 0.12mol / L, acetic acid-sodium acetate buffer solution with a pH of 5.4, then add the compound enzyme powder, and stir well; enzymolyze at 45°C for 1.5 hours, then add 900mL of alcohol, and stir thoroughly for 10 minutes; Filter through a 4-micron filter to remove the residue, extract the filter residue twice with alcohol, combine the extracts and concentrate them with a rotary evaporator (at a temperature of 52°C, at a speed of 30r / min) to form an extract. During the concentration process, ethanol is recovered. Finally, the extract is vacuum freeze-dried to obtain the gastrodin extract.

[0019] The yield of gastrodin deter...

example 3

[0020] Example 3. (Gastrodia elata was purchased from the Yunnan Agricultural Products Expo)

[0021] Weigh 5.0g of cell wall-breaking enzyme, 4.0g of amylase, 4.0g of pectinase, and 1.0g of protease and mix them for later use; crush the naturally dried Gastrodia elata through a 20-mesh sieve, weigh 200g, and dissolve in 500mL at a concentration of 0.16mol / L, acetic acid-sodium acetate buffer solution with a pH of 4.8, then add the compound enzyme powder, and stir well; enzymolyze at 55°C for 2 hours, then add 1500mL of alcohol, and stir for 12 minutes; Filter through an 8-micron filter to remove the residue, extract the filter residue twice with alcohol, combine the extracts, concentrate them into an extract by rotary steaming at a temperature of 60°C, recover ethanol during the concentration process, and finally dry the extract naturally to obtain Gastrodin Extract.

[0022] The yield of gastrodin determined by HPLC was 0.36%, while the yield of gastrodin extracted from the...

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Abstract

The invention provides a method for extracting gastrodin by an enzyme method. The method is characterized by comprising the following steps: dissolving rhizoma gastrodiae powder in acetic acid-sodium acetate buffer solution and mixing the two substances evenly; adding complex enzyme into the solution for stirring evenly and enzymolysis; adding alcohol into the mixture to for sufficiently stirring, filtering and deslagging; concentrating extracting solution into extract by a rotary steaming method; and drying the extract to obtain the gastrodin extract. The method has the advantages that as high efficiency and specificity of biological enzyme and mild acting condition, compared with an ultrasonic extracting process, the extracting condition is more convenient, and the equipment is more simplified, so that energy consumption is reduced, and the cost is saved; compared with a hot alcohol refluence extracting process, the extracting condition is simple, the temperature of an extracting system is reduced, so energy consumption of heating is reduced, the pollution to the environment is reduced, a micromolecular drug effect ingredient with thermal volatilization can be remained, and the drug effect of the obtained gastrodin is more complete; and compared with the prior art, the extracting rate is improved by 0.05 to 0.13 percent.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a method for extracting gastrodin from Gastrodia elata by using biological enzyme technology. Background technique [0002] Gastrodia elata (Gastrodiaelata Blame) is a plant of the orchid family. The dry tubers of Gastrodia elata have been used medicinally in my country for more than 2,000 years. , efficacy and clinical application are discussed in great detail. "National Compilation of Chinese Herbal Medicine" summarizes the efficacy of Gastrodia elata as follows: treating high blood pressure, dizziness, headache, crooked mouth and eyes, numbness of limbs, convulsions in children and other diseases. Foreign studies have found that Gastrodia elata has a good preventive and therapeutic effect on senile dementia. In Chinese and Western medicine clinics in my country, Gastrodia elata is mainly used to treat the following diseases: convulsions, convuls...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/20C07H1/08A61P25/20
Inventor 陈卫锋徐升运马齐任平赵文娟秦涛
Owner ENZYME ENG INST SHAANXI PROVINCE ACAD OF SCI
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