150results about How to "Moderate working conditions" patented technology

The invention discloses a method for extracting tea seed oil by using an enzymatic hydrolysis method, which comprises the following steps of: airing, unshelling, husking and pulverizing tea seeds; carrying out enzymolysis on the tea seeds in the material-liquid ratios of 1:3 to 1:5 at the extraction temperature of 40-60 DEG C for 1-5h with the enzyme quantity of 40-600U under the pH natural condition; centrifugally layering; adding an organic solvent into centrifugal liquid and extracting; and rotating and evaporating to obtain an oil sample. By adopting the scheme, the method has the advantages of low temperature for equipment operation, low energy consumption, high purity of extracted oil, low content of phospholipid, low acid value and low peroxide value, shallow color, low BOD (Biochemical Oxygen Demand) and COD (Chemical Oxygen Demand) values, easiness of treatment and less pollution, and the oil yield ratio is up to 23.1 percent.

The invention relates to a pretreatment process of fabrics in the printing and dyeing industry, and in particular relates to a process for pre-treating fabrics by a bio-enzymatic method. The method comprises the following steps: padding for the first time; carrying out a cold pad-batch process on the fabrics; sequentially washing with hot water and cold water, and squeezing; padding for the second time; steaming the fabrics; and washing with hot water, then washing with cold water once and drying, wherein, a complex enzyme system is added in the procedure of padding for the first time, and the complex enzyme system comprises alkali pectinase, amylase and lignin degrading enzyme systems. By utilizing the process, the biological enzyme is added to the fabrics for synchronously desizing, refining and bleaching the fabrics; the process has the advantages of moderate action conditions, no damage on the fabrics, energy loss reduction and environmental friendliness; and the wettability, impurity removal rate and whiteness of the obtained fabrics are much higher than those of the fabrics obtained through alkali treatment.

The invention provides a method for improving the physical and mechanical properties of oxidation-degummed ramie. The method comprises the following steps of: preparing a degumming solution by using caustic soda, an oxidant, a surfactant, a fiber expanding agent, an oxidant stabilizer and water; mixing raw ramie and the degumming solution, heating, and boiling off to sufficiently remove colloid; and then cooling, adding a reduction softener, maintaining the temperature, oiling and drying after washing with clear water, and opening and carding to obtain textile ramie fiber subjected to reduction modification. According to the method disclosed by the invention, the defects of the prior art are overcome; and the reduction softener is added to raffinate obtained after oxidation-degumming, oxycellulose produced in the degumming process is reduced and softened, so that the physical and mechanical properties, such as strength, length, elongation and softness of the fiber are improved, the working procedures are simplified, and pollution and environmental pressure are lightened.

The present invention relates to re-compounding and application in cotton fabric treatment of enzyme preparation, and is especially enzyme process for desizing, boiling off and bleaching cotton fabric. Different enzyme preparation is first re-compounded with alpha-amylase, PVA degrading enzyme, lipase, proteinase, alkaline pectase, xylanase, cellulase, sugar oxidase, etc based on different fabric pre-treating requirements; and then used in desizing, boiling off and bleaching cotton fabric to replace traditional concentrated alkali solution and high temperature chemical process. The present invention has low power consumption and environment friendship.

The invention discloses a sulfur-nitrogen-doped porous biochar, a preparation method and an application. The preparation method uses calcium sulfate as a template, potassium oxalate as an activator and bean fibers as a carbon source, and employs calcination to prepare the sulfur-nitrogen-doped porous biochar. The sulfur-nitrogen-doped biochar is an environment-friendly adsorbent for treating heavymetal wastewaters with low cost and high efficiency, adsorption capacities for Cu<2+> and Ni<2+> ions are substantially superior to other biochars. At the same time, the sulfur-nitrogen-doped biocharcontaining adsorbed heavy metals can be subjected to a reduction treatment, and then the reduction-treated sulfur-nitrogen-doped biochar can be further used for catalytic degradation of pollutants inwater to realize recovery and application of the heavy metals.

The invention discloses a method for extracting grease from oil-tea camellia seed kernel and recovering saponin and saccharide active substances, comprising the following steps of: (1) crushing and tempering: with hull-removed oil-tea camellia seed kernels as raw materials, crushing and grinding the materials, adding water for uniformly stirring, wherein the quality-volume ratio of the oil-tea camellia seed kernels to the water is 1kg:2-8L and controlling the temperature of the system to be 30-60DEG C; (2) carrying out the enzymolysis: adjusting the pH value of the system to be 3.5-8.0, adding pectinase the weight of which is 0.1-2 percent that of the camellia seed kernels and carrying out the enzymolysis for 2-5h; (3) heating and centrifuging: treating the system at the temperature of 80-90DEG C for 5-15min after enzymolysis; and (4) recovering the grease and the active substances: in which, the centrifuged and separated system comprises boiled oil, liquid phase and residue, taking out the boiled oil and spray-drying the liquid phase to obtain the active substances containing the saponin and saccharide. The method for extracting the grease avoids the high-temperature pretreatment of high-temperature enzymolysis of oil materials and can be used for synchronously recovering oil and a saponin and saccharide mixture which can be used as a saponin crude product or a feed additive.

The invention provides a preparation method of wall-breaking enzymolysis bee pollen. The preparation method is characterized by specifically comprising the following steps: 1) removing impurities; 2) sterilizing; 3) mechanically breaking the wall; and 4) carrying out biological enzymolysis. The preparation method of the wall-breaking enzymolysis bee pollen has the following advantages that (1) the wall-breaking effect is remarkable, and the benefits are provided for releasing and separating the nutritional components in the bee pollen such as the fatty acid, flavonoid and polysaccharide; (2) the sensitization of antigen protein is removed, the enzymolysis is carried out on alkaline glutelin, and the bioavailability is improved; (3) the dietary fibers such as lignin are obviously reduced, the absorption performance of animals to liposoluble substances is improved, the energy utilization rate is raised, and the animal production achievement is improved; (4) the particles are fine, good in flavor and comfortable in taste, and the animal ingestion and favorite are obviously improved; and (5) the action condition is mild, and little damage is brought to the heat sensitive nutritional substances.

The invention relates to a method for preparing a fish-skin collagen powder by the use of fresh fish skin. The method comprises the following steps: fresh fish skin is selected as a raw material and is immersed in sodium hydroxide, the fresh skin is washed with water to neutral pH after fat and impure protein on the surface of the fish skin are removed, and crushing is carried out; drinking water and edible vinegar are added to the crushed fish skin and uniform agitation is carried out, ultrasonication and deodorization treatments are carried out, and papain is added to carry out controlled-enzymatic hydrolysis so as to obtain a fish-skin enzymatic hydrolysate; active carbon is added for decarburization clarification after enzyme deactivation, filtration is carried out, and a filtrate undergoes concentration and spray drying so as to obtain the fish-skin collagen powder. By ultrasonication of the fish skin, active ingredients in acetic acid are activated to react with fishy smell substances in the fish skin so as to remove the fishy smell. By adding active carbon into the enzymatic hydrolysate to carry out decarburization clarification for fishy smell elimination and decoloration, the prepared fish-skin collagen powder has no fishy smell. The method provided by the invention has advantages of advanced technology, reasonable process and controllable operation, and is suitable for large-scale production.

The present invention discloses a cleaner production method for synthetizing chloromethane. The method comprises the steps: 1), synthetizing chloromethane: synthetizing crude chloromethane from gas-phase hydrogen chloride and gas-phase methanol; 2), recovering waste heat: performing heat exchange on the crude chloromethane by a heat exchange device and liquid methanol, cooling and discharging, wherein liquid methanol is heated by the heat exchange device to form gas-phase methanol which enters the step 1) as a raw material to react; 3), deacidifying by a deacidifying tower: deacidifying the crude chloromethane; 4), refining in an absorption tower; 5), pressurizing, extracting, rectifying and removing ether; and 6) performing diluted acid solution membrane concentration. In the method, the pressure application ranges of the hydrogen chloride and the methanol are relatively wide, and the content of HCl in diluted acid solution is greatly reduced; the methanol is used as a washing solution, other secondary wastes can not be generated; raw materials, such as the methanol and HCl, are utilized to the greatest extent; the unit energy consumption of CH3Cl is greatly reduced, the unit energy consumption of a product, namely CH3Cl is reduced by more than 20% compared with that in an industrialized unit in the prior art.

The invention discloses a method for separating and purifying Natto kinase mainly using ion exchange chromatography. The steps are composed of crude enzyme liquid preparation, gel chromatography and ion exchange chromatography, activating bacillus subtilis through slant medium, medium culture and fermenting in ferment medium, natto kinase being secreted to fermented solution by bacillus subtilis, centrifuging to collecting upper clear solution, then proceeding (NH#-[4])#-[2]SO#-[4] graded precipitation, redissolving using phosphorus acid buffer, adding redissolved solution to gel chromatography column to proceeding gel filtration, collecting elution peak containg natto kinase, adding fraction of having natto kinase activity from gel filtration to inon exchange chromatography column, proceeding adsorbing, collecting elution peak containg natto kinase, freeze drying to get purified natto kinase. The invention has merits of condition, low cost, simple process , easy operation ,high purity and short production period, etc.

The invention relates to a process for preparing corn peptides by taking corn starch sugar residues as raw materials. The process comprises the following steps of: crushing corn starch sugar residues, adding absolute ethyl alcohol to extract a yellow pigment, drying the materials subjected to complete pigment extraction, crushing and sieving, adding water, adjusting the pH value, and respectively adding alkaline protease and flavourzyme to hydrolyze; adjusting the pH value by using hydrochloric acid and adding active carbon to decolor and remove bitter; desalting the obtained feed liquid by using a membrane system, and freezing and drying in vacuum to prepare corn peptides powder. According to the process, the corn starch sugar residues are used as the materials to prepare the corn peptides, and the process is low in cost, material consumption and energy consumption, and is beneficial to the improvement of resource utilization of by-products.

The invention belongs to the technical field of oil remediation, and particularly relates to an organophosphorus pesticide contaminated soil remediation agent. The organophosphorus pesticide contaminated soil remediation agent is prepared from chitosan, bentonite, green algae, citric acid, shell powder, needle mushroom residues, corn cobs, rice bran, tea seed shells, walnut shells, areca sawdust, palm sawdust, a composite enzyme preparation and a compound microbial agent. According to the soil remediation agent, under the condition that no other chemical solvent is introduced, the mild enzyme and the microbial agent are adopted to soil treatment, secondary pollution is not produced, and action conditions are mild.

The invention discloses a preparing method of a swim bladder protein beverage, belonging to the technical field of aquatic product processing. The swim bladder protein beverage uses dry swim bladder as raw material and is produced after the preparation, the enzymatic hydrolysis, the debiting and deodourizing, the blending, the can filling and the sterilizing of swim bladder slurry. Enzyme is adopted for the controllable degradation of the swim bladders. The reaction conditions are mild. Few nutritional components are lost. A compound method is adopted for debiting and deodourizing enzyme solution. And the special masking function of natural products is also adopted. After the flavoring, the swim bladder protein beverage with perfect taste and flavor and both nutritional and healthy values is obtained. The preparing method of the swim bladder protein beverage improves the processing level and added value of the swim bladders, eliminates poor flavors of swim bladder protein degradation products of fishy smell, bit, and the like and can be applied to the industrialized production. The beverage has the advantages that the product nutritional value is high, the digestion and absorption are easy and the drinking is convenient.

The invention discloses a preparation process of red date condensed juice high in cAMP content. The preparation process includes the following steps of firstly, conducting separating, washing and smashing; secondly, conducting hot water extraction; thirdly, conducting cooling and heat preservation enzymic method oriented conversion; fourthly, adding composite enzyme preparations; fifthly, inactivating enzymes; sixthly, conducting centrifugation; seventhly, conducting filtration; eighthly, conducting concentration and pasteurization; ninthly, conducting filling. Compared with the prior art, the preparation process has the advantages that the process is simple, the production period is shortened, and the energy loss is reduced; extraction is conducted after smashing is conducted, and therefore extraction of nutritional ingredients in red dates can be improved; by means of the extraction and enzymolysis combined method, the juice yield is greatly improved, the processing time is greatly shortened, and the cyclic adenosine monophosphate content in red date juice is improved; date peel is utilized, and the cAMP content is improved; preparations of pectinase, cellulose and protease are used in a composite mode, and the hydrolysis capacity of enzymic preparations is improved; the step-by-step enzymolysis and extraction process is adopted, and interference between related enzyme preparations is avoided.

The invention provides a method for in-situ construction of a surface coating layer based on a metal-organic network framework material, which promotes in-situ growth of nanoparticles on the surface of a core structure by regulating and controlling the dynamic process, namely nucleation and growth, of a metal organic network framework structure so as to achieve the purpose of constructing a uniform coating layer on the surface of a core. By adopting the method provided by the invention, a coating layer based on a metal organic network framework material can be constructed on the surface of a conventional positive electrode material, and the prepared positive electrode material can effectively inhibit the side reaction between the electrode material and an electrolyte, slow down the generation of surface SEI, reduce the surface film impedance and charge transfer impedance, and improve the reaction kinetic process of lithium ions; meanwhile, dissolution of transition metal ions can be inhibited, collapse of a lattice structure in the circulation process of the electrode material is effectively relieved, and the circulation stability and the rate capability of the positive electrode material are remarkably improved.

This invention relates to preparation of deer peptide with biological activity by composite protease catalysis and its uses in human immunity, fatigue resistance and hypoxia tolerance. Products are obtained by heating deer meat paste as raw material, which is screened with 80 mesh, to 48-52 deg.C, regulating pH 6.8-7.2 with NaOH solution, stirring and adding neutral protease, papoid or trypsin, then adding fragrant protease after protein hydrolysis to 28-33%, controlling reaction with standard 2M NaOH to make protein hydrolysis to 34 -42%, heating enzymolysed liquid to 114-116 deg.C for 10-15 minutes, sharply cooling to atmosphere, solid-liquid separating, concentrating, and drying to obtain yellow powder or granular products of deer peptide.

The invention belongs to the technical field of soil restoration and relates to a polycyclic aromatic hydrocarbon-polluted soil restoration material. The polycyclic aromatic hydrocarbon-polluted soil restoration material comprises chitin, zeolite powder, green alga, humic acid, shell powder, needle mushroom residues, cob, rice bran, tea seed shells, peat ash, betel nut tree wood chips, palm tree wood chips, a compound enzyme preparation and composite microbe inocula. The polycyclic aromatic hydrocarbon-polluted soil restoration material utilizes mild enzymes and a microbe bacterial strain to treat soil without use of other chemical solvents so that novel pollution is avoided and action conditions are mild. The strain degrades or adsorbs polycyclic aromatic hydrocarbons but also obviously improves soil quality conditions.

The invention provides a method for treating antibiotic residues by an enzymic method. The method comprises the following steps: mixing an enzymic preparation combination with water according to a volume ratio of 1:50 to obtain an enzymic preparation combination solution; uniformly spraying the enzymic preparation combination solution onto fresh antibiotic residues by a sprayer, and stirring for 0.5-1h mechanically, wherein 1-5L of the enzymic preparation combination solution is added to each ton of antibiotic residues; putting the well stirred antibiotic residues to a storage tank, and reacting for 1-4h at the temperature of 30-50 DEG C under the condition of natural pH value, wherein the antibiotic residues are at a natural solid-liquid separated state at the end of the reaction, supernate can be used for biogas fermentation, and solids are used for preparation of organic fertilizers. By the method disclosed by the invention, pollution to the earth surface and air, caused by the emission of cassava waste residues, can be solved to a great extent, and dual effects of reutilization of waste residues and energy conservation and emission reduction can be achieved.

The invention relates to the technical field of food processing, in particular to a preparation method of zein-bee pollen extract microcapsules. According to the specific technical scheme, the preparation method comprises the following steps of taking a bee pollen extract as a core, taking zein as a shell, adding the bee pollen extract to a zein solution, performing ultrasonic dissolving, and performing dispersing so as to obtain a zein-bee pollen extract solution; and then performing granulation by an electrostatic spraying method so as to obtain the zein-bee pollen extract microcapsules. Through the preparation method disclosed by the invention, the problems that in the prior art, effective components cannot be released in the intestines and the stomach, prepared microcapsules are unevenin granules, and the encapsulation efficiency is low are solved.

The invention relates to the field of detection of cultural relics and discloses a method for identifying the varieties of residual silk of antique silk fabric based on an immune trace method. The method comprises the following steps: preparing a fluorescent carbon point by taking a sweet potato as a carbon source, marking secondary antibodies of mulberry silk and tussah silk by using the fluorescent carbon point, hydrolyzing promiscuous antique silk fabric cultural relic samples by using ionic liquid and PM13-alkaline protease step by step, performing dialysis and purification after protein extracting liquid is obtained, performing SDS-PAGE gel electrophoresis, transferring the obtained protein band to a PVDF membrane, incubating silk primary antibodies and the secondary antibodies marked by the fluorescent carbon point, observing the immunofluorescence band in a gel imaging system, and identifying the varieties of the antique silk fabric. According to the method, the use amount of a chemical reagent is small, and the reaction is mild, environment-friendly and harmless; and when the antique silk fabric is detected, the method has the characteristics of small use amount of samples, intuitive performance, rapidness and high sensitivity.

The invention belongs to the technical field of soil remediation, and in particular relates to a polycyclic aromatic hydrocarbons-contaminated soil remediation agent. The polycyclic aromatic hydrocarbons-contaminated soil remediation agent is prepared from the following components: chitin, zeolite powder, green algae, humic acid, shell powder, mushroom bran of flammulina velutipes, corncob, pulverized fuel ash, tea seed shell, peat ash, areca sawdust, palm sawdust, a complex enzyme preparation and a composite microbial agent. After the soil remediation agent is adopted, the soil is treated by using mild enzymes and microbial strains under the condition that other chemical solvents are not introduced into the soil, so that the secondary pollution is not caused, and the action condition is mild.

The invention relates to a method for improving cohesiveness of a hemp / resin composite material interface, and belongs to the technical field of preparation or chemical treatment of modified celluloseor other cellulose derivatives. The method includes: placing a hemp fabric in a buffering solution which contains laccase, TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl) and fatty amine monomers, enabling hydrophobic exogenous amine monomers to be grafted on xylogen and cellulose on a surface of the hemp fabric, taking out fibers after reaction is over, fully washing with water, removing unreactedmonomers absorbed on the hemp fibers, and drying at a room temperature. By applying the method to mechanical property treatment of the composite material, excellent surface hydrophobicity of the hempfibers is provided, and interface cohesiveness and compatibility between the fibers and a matrix in a hemp-enhanced resin matrix composite material are improved.

The invention belongs to the technical field of soil remediation and particularly relates to a remediation material for soil contaminated by organophosphorus pesticides. The remediation material is prepared from the following components: chitin, kaolin, green algae, citric acid, shell powder, golden needle mushroom bran, corn cobs, rice bran, tea seed shells, walnut shells, areca catechu tree wood chips, palm tree wood chips, a complex enzyme preparation and a complex microbial agent. With the adoption of the soil remediation material, the soil is treated by mild enzymes and microbial strains without introduction of other chemical solvents, and therefore, secondary pollution is avoided, and the acting condition is mild.

The invention relates to an extraction method of effective components of Chinese angelica. The extraction method comprises the following steps: 1, Chinese angelica is crushed; 2, crushed Chinese angelica is added to a solvent A solution with the pH value of 5.5; 3, cellulase with the concentration of 0.3mg.mL<-1> is added to the solution obtained in step 2; 4, the solution obtained in step 3 is subjected to enzymatic hydrolysis for 6h at a constant temperature of 55DEG C; 5, isoamylase with the concentration of 0.3mg.ml<-1> is added to the solution obtained in step 4; 6, a solution obtained in step 5 is subjected to enzymatic hydrolysis for 6h at a constant temperature of 55DEG C; 7, the solution obtained in step 6 is subjected to reduced pressure filtration after completing the enzymatic hydrolysis, and the obtained filtrate is preserved in a dark place; 8, the filter residue obtained by filtering in step 7 is used for extracting ligustilide, an eight time solvent B is added each time, reflux extraction is twice carried out for 2h, the obtained first filtrate and the obtained second filtrate are mixed to obtain a mixed filtrate which is a ligustilide solution, and the ligustilide solution is preserved in a low temperature dark place; 9, the filter residue obtained in step 8 is used for extracting angelica polysaccharides, an fifteen time solvent C is added to the filter residue to extract; 10, the filtrate obtained in step 9 is concentrated to obtain a concentrate; and 11, a solvent D is added to the concentrate obtained in step 10 until that the concentration of the solvent D reaches 75% to obtain a deposition, and the deposition is centrifuged by a centrifuge to obtain the angelica polysaccharides.

The invention provides a composite microbial preparation, which belongs to the technical field of soil remediation, and in particular relates to a composite microbial preparation for remediating polycyclic aromatic hydrocarbon-contaminated soil. The microbial preparation in the present invention contains microorganisms of different strains, and has dual functions of repairing the soil ecosystem and degrading polycyclic aromatic hydrocarbons. The composite microbial preparation provided by the invention also includes a soil conditioner, which can improve the permeability of the soil and increase the oxygen transmission in the soil, thereby quickly establishing a healthy microbial population structure. In addition, the compound microbial preparation of the present invention is a dry powder, which is easy to store and activate, and has a long shelf life. Microbial strains treat the soil without secondary pollution, and the action conditions are mild.

The present invention belongs to the technical field of soil remediation and discloses a polycyclic aromatic hydrocarbon contaminated soil remediation material. The polycyclic aromatic hydrocarbon contaminated soil remediation material comprises the following components in parts by weight: diatomaceous earth, peat soil, humic acid, corn cob, nutrients, a surfactant, an oxidant and a compound microbial agent; the nutrients comprise rice bran, (NH4)2HPO4, urea, calcium superphosphate and plant ash; the surfactant is an anionic and non-ionic mixed surfactant; the oxidant is one of hydrogen peroxide activated sodium persulfate, potassium hydrogen persulfate or a Fenton reagent; the compound microbial agent comprises pseudomonadaceae powder, bacillus powder and fusarium powder; and the weight ratio of the pseudomonadaceae powder, bacillus powder and fusarium powder is 3:(1-5):(2-5). Compared with the prior art, beneficial effects are as follows: the polycyclic aromatic hydrocarbon contaminated soil remediation material is capable of degrading polycyclic aromatic hydrocarbons in the soil and also has an improved effect on the soil conditions of the soil.

The invention discloses a preparation method and an application method for a soil remediation material. Chitosan, talcum powder, dunaliella, citric acid, shell powder, needle mushroom residue, corncobs, rice bran, bamboo charcoal, plant charcoal ash, areca wood chips and coconut shells are stirred to be uniformly mixed, then added with a compound microorganism preparation and packaged into bags, and thereby the soil remediation material is prepared; and the compound microorganism preparation consists of pseudomonas aeruginosa powder, pseudomonas fluorescens powder, shewanella powder, trichoderma reesei powder and bacillus mucilaginosus powder. Compared with the prior art, the soil remediation material disclosed by the invention has the characteristic of plant and microorganism synergism.

The invention relates to a process for carrying out pretreatment on fabrics in printing and dyeing industries, in particular relating to a process for simultaneously carrying out desizing, scouring and bleaching on fabrics. The process provided by the invention comprises the following steps of primary padding, fabric cooling in pile, hot water rinsing, clod water rinsing, squeezing, second padding, fabric steaming, hot water rinsing, clod water rinsing, and drying. Because the fabrics are pretreated by using the process, the energy consumption is reduced, and the process is environment-friendly; and because in the process, biological enzymes are adopted to act on the fabrics, the action has a mild condition and causes no damage on the fabrics, and the wettability, impurity removal rate and whiteness of the obtained fabrics are much higher than those of the fabrics subjected to alkali treatment.

The invention discloses a biological disinfectant in a food field. The biological disinfectant belongs to the field of food disinfection, and comprises the following components in part by weight: 0.5 to 1 part of sodium dichloro cyanurate, 5 to 10 parts of tween-20, 1 to 5 parts of ethylenediaminetetraacetic acid, 1 to 10 parts of compound phosphate, 5 to 10 parts of ethanol, 100 parts of distilled water, and 5 to 10 parts of sorbitol. The biological disinfectant also comprises 0.01 to 0.1 weight percent of lysozyme. The biological disinfectant in the food field has the advantages of strong sterilization specificity, mild working conditions and quick effect, and drug resistance of strains can be prevented. The biological disinfectant is soluble in water, does not irritate articles, and can be used safely. Particularly, the biological disinfectant does not produce harmful substance residues in food. The biological disinfectant, which is simple in preparation, is applied to the surface disinfection and cleaning of the food processing equipment, food equipment and food processing environment, and fruits and vegetables and common articles in household life.