Compound microorganism preparation
A technology of composite microorganisms and microorganisms, applied in the field of soil remediation, can solve problems such as soil pollution, achieve the effects of increasing transmission volume, good repair effect, and improved permeability
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Embodiment 1
[0028] Example 1: Enrichment of microbial flora degrading benzopyrene
[0029] The soil samples come from areas polluted by polycyclic aromatic hydrocarbons, and the sampling method is carried out by the five-point sampling method, with 200g of samples taken at each point, and a total of 1000g of samples are taken, which are mixed for testing.
[0030] Prepare benzopyrene inorganic salt medium: prepare 10g / L benzopyrene solution with acetone, sterilize with a 0.25mm filter membrane, measure a certain amount of benzopyrene solution and place it in a sterilized Erlenmeyer flask until the acetone is volatilized Then add an appropriate amount of sterilized inorganic salt basal medium, so that the final concentration of benzopyrene is 1500 mg / L.
[0031] Take 6 triangular flasks, place 150ml of liquid inorganic salt medium containing benzopyrene in each triangular flask, take 5 of the above triangular flasks (A1-A5), add 1g of soil sample to each flask, and add sterilized 1g of wa...
Embodiment 2
[0034] Example 2: Isolation, identification and acquisition of microbial flora degrading benzopyrene
[0035] As can be seen from the results in Table 1, the A2 and A4 culture fluids contain the dominant flora that degrades benzopyrene. Take 0.1ml of the above-mentioned A2 and A4 culture fluids, dilute them in a certain ratio, and apply them to the inorganic salt solid containing benzopyrene. On the culture medium, single colonies with different characteristics were picked, purified and inoculated on the inorganic solid salt medium containing benzopyrene, cultured and selected with a faster growth rate, 16S rDNA was extracted by alkaline lysis method and 16S rDNA Sequence analysis (sequence analysis was completed by Shanghai Meiji Biomedical Technology Co., Ltd.), BLAST comparison was performed on the sequencing results, and 15 strains with high degradation of benzopyrene were obtained. The names of the strains are shown in Table 2.
[0036] Table 2 Names of benzopyrene-degrad...
Embodiment 3
[0051] Soil conditioner can break soil compaction, loosen soil, improve soil air permeability, reduce soil bulk density, promote soil microbial activity, enhance soil fertilizer and water penetration; improve soil, enhance crop disease resistance, increase crop yield, improve agricultural product quality, restore The original ecology of crops and other functions. The soil conditioner provided by the invention is mainly used to improve the permeability of the soil and increase the oxygen transmission amount in the soil, so as to quickly establish a healthy microbial population structure.
[0052] The composition of the compound microbial preparation provided by the invention comprises by weight
[0053] Component A (soil conditioner): 35 parts of oyster shell fine powder, 15 parts of straw biomass coke, 14 parts of phosphate rock powder, 25 parts of ammoniated sludge, 0.1-0.2 parts of decomposing agent, 3-6 parts of gypsum powder;
[0054] Component B (microbial mixture): 0.1 ...
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