Physiological active buck peptide by compound proteinase catalysis and use of products thereby

A composite protease, physiologically active technology, applied in the direction of peptide/protein components, medical preparations containing active ingredients, drug combinations, etc., can solve problems such as reducing application value

Inactive Publication Date: 2004-12-08
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, venison has only been used as a low-fat and high-protein delicacy, which greatly reduces its application value

Method used

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  • Physiological active buck peptide by compound proteinase catalysis and use of products thereby
  • Physiological active buck peptide by compound proteinase catalysis and use of products thereby
  • Physiological active buck peptide by compound proteinase catalysis and use of products thereby

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0101] Example 1: The technological process refers to the attached figure 1

[0102] Put 80 kilograms of pretreated venison pulp into the reaction tank, stir and heat to 50° C., and adjust the pH value to 7.0. During stirring (rotating speed 120rpm / min), first add the neutral protease dissolved in ice water, control the reaction process with standard NaOH solution, and make the degree of protein hydrolysis reach 28%, then add the flavor enzyme dissolved in ice water to continue the hydrolysis reaction to make the protein When the degree of hydrolysis reaches 38%, the temperature of the enzymolysis solution is kept at 115°C for 15 minutes, and then the temperature is quickly lowered to room temperature. Concentrate under reduced pressure to a solid content of 25%, and then freeze-dry. Promptly obtain about 8 kilograms of deer peptides with the physiological function of improving immunity (the protein recovery rate is about 70%).

example 2

[0103] Example 2: The technological process refers to the attached figure 2

[0104] Put 80 kilograms of pretreated venison pulp into the reaction tank, stir and heat to 50° C., and adjust the pH value to 7.0. During stirring (rotating speed 120rpm / min), first add the trypsin dissolved in ice water, control the reaction process with standard NaOH solution, make the degree of protein hydrolysis reach 33%, then add the flavor enzyme dissolved in ice water to continue the hydrolysis reaction, so that the protein When the degree of hydrolysis reaches 41%, the temperature of the enzymolysis solution is kept at 115°C for 15 minutes, and then the temperature is quickly lowered to room temperature. Concentrate under reduced pressure to a solid content of 25%, and then freeze-dry. Promptly obtain about 8.5 kilograms of deer peptides with hypoxia resistance (the protein recovery rate is about 72%).

example 3

[0105] Example 3: The technological process refers to the attached image 3

[0106] Put 80 kg of pretreated venison pulp into the reaction tank, stir and heat to 50°C, and adjust the pH value to 7.0±0.1. During stirring (rotating speed 120rpm / min), first add the papain dissolved in ice water, control the reaction process with standard NaOH solution, make the degree of protein hydrolysis reach 31%, then add the flavor enzyme dissolved in ice water to continue the hydrolysis reaction, so that the protein When the degree of hydrolysis reaches 42%, the temperature of the enzymolysis solution is kept at 115° C. for 15 minutes, and then the temperature is quickly lowered to room temperature. Concentrate under reduced pressure to a solid content of 25%, and then freeze-dry. Promptly obtain about 8 kilograms of deer peptides with anti-fatigue physiological function (the protein recovery rate is about 70%).

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PUM

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Abstract

This invention relates to preparation of deer peptide with biological activity by composite protease catalysis and its uses in human immunity, fatigue resistance and hypoxia tolerance. Products are obtained by heating deer meat paste as raw material, which is screened with 80 mesh, to 48-52 deg.C, regulating pH 6.8-7.2 with NaOH solution, stirring and adding neutral protease, papoid or trypsin, then adding fragrant protease after protein hydrolysis to 28-33%, controlling reaction with standard 2M NaOH to make protein hydrolysis to 34 -42%, heating enzymolysed liquid to 114-116 deg.C for 10-15 minutes, sharply cooling to atmosphere, solid-liquid separating, concentrating, and drying to obtain yellow powder or granular products of deer peptide.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a deer peptide product with physiological activity prepared by hydrolyzing venison with a method catalyzed by a compound protease, and the deer peptide product can improve immunity of the body, enhance fatigue resistance and hypoxia resistance, etc. aspects of application. Background technique [0002] Physiologically active peptides are the most popular research topics in the current food science field. They are generally produced from proteins through enzyme action. Different products can be produced due to different raw protein, hydrolytic enzymes, and separation and refining methods; peptides, especially some oligopeptides Not only has better digestion and absorption performance than protein, the most exciting thing is that some peptides have been found to have physiological activities such as regulating human physiological functions, preventing and resisting diseases,...

Claims

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Application Information

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IPC IPC(8): A61K38/02A61P37/04A61P43/00C12P21/00
Inventor 冯雁张作明马吉胜李媛媛韩四平
Owner JILIN UNIV
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