48results about How to "Low in phospholipids" patented technology

The invention discloses a method for extracting tea seed oil by using an enzymatic hydrolysis method, which comprises the following steps of: airing, unshelling, husking and pulverizing tea seeds; carrying out enzymolysis on the tea seeds in the material-liquid ratios of 1:3 to 1:5 at the extraction temperature of 40-60 DEG C for 1-5h with the enzyme quantity of 40-600U under the pH natural condition; centrifugally layering; adding an organic solvent into centrifugal liquid and extracting; and rotating and evaporating to obtain an oil sample. By adopting the scheme, the method has the advantages of low temperature for equipment operation, low energy consumption, high purity of extracted oil, low content of phospholipid, low acid value and low peroxide value, shallow color, low BOD (Biochemical Oxygen Demand) and COD (Chemical Oxygen Demand) values, easiness of treatment and less pollution, and the oil yield ratio is up to 23.1 percent.

The related preparation method for oil and oil protein comprises: cleaning material, drying till water content in 2-10%; cooling to ambient temperature, peeling or shelling more than 90%, separating core and seedcase to obtain the oiling seed with 0.5-10% seedcase; after rolling or not, expressing with low-temperature device at 20-70Deg to obtain crude oil and cake; depositing, fine filtering to obtain the objective oil; super grinding the cake to prepare semi-degreased protein powder, or soaking to obtain leachate for recovery and wet dreg to recover ethanol and grind for protein powder.

A method for degumming an oil composition comprises the steps of (a) providing an oil composition containing a quantity of phospholipids, (b) contacting said oil composition simultaneously with one or more phospholipase A enzymes and one or more phospholipase C enzymes, under conditions sufficient for the enzymes to react with the phospholipids to create phospholipid reaction products, and (c) separating the phospholipids reaction products from the oil composition, the remaining oil composition after the separation being a degummed oil composition, whereby during step (b) the reaction of said one or more phospholipase A enzymes proceeds at a faster rate than it would in the absence of said one or more phospholipase C enzymes, and wherein the reaction of step (b) continues for a duration of less than about one hour.

The invention discloses a preparation method of rapeseed oil. The preparation method comprises the steps as follows: rapeseeds are squeezed and filtered to obtain crude oil and an oil squeezed cake; the crude oil is put into an oil refining caldron and stirred till no bubbles exist, and the temperature of the oil refining caldron is in a range from 50 EDG C to 95 DEG C; warm water with the crude oil weight of 5-10% and edible non-iodized salt with the crude oil weight of 4-5% are added into the crude oil for hydration; after hydration, sedimentation is performed, and water and colloidal impurities subsided at the bottom of the crude oil are removed to obtain raw oil; the raw oil is dried, and water in the raw oil is removed; the temperature and the vacuum degree for drying are maintained, an adsorbent and fennel are added in the raw oil for decoloration, and the total adding amount of the adsorbent and the fennel accounts for 1-3% of the mass of the raw oil; and the decolored raw oil is filtered to obtain the rapeseed oil. The invention further discloses the rapeseed oil prepared with the preparation method. All indexes of the rapeseed oil all meet the international requirements of the four-level rapeseed oil, and intense original rapeseed oil fragrance can be maintained.

The invention discloses a grease refining method based on the enzymatic degumming technology. Grease is subjected to acid reaction, then enzymatic degumming is conducted, and degummed oil is obtained through colloid separation, wherein the phosphorus content of the degummed oil is not higher than 30 mg/kg; the degummed oil is subjected to alkali refining to obtain desaponification oil, the desaponification oil is subjected to decoloration and deodorizing, and refined oil is prepared. The method reduces the phospholipid content in soapstock and improves quality of acidic oil; the desaponification oil with lower phosphorus content can be obtained; phospholipid in grease is separated independently, and high-value application is further facilitated.

The invention discloses a refining method for fourth-grade rapeseed oil. The refining method comprises the following steps: (1) carrying out pre-acidification; (2) carrying out post-acidification; (3) carrying out hydration; (5) carrying out centrifugal degumming; (5) carrying out oxidative decolorization; and (6) carrying out vacuum dehydration and desolvation. According to the method disclosed by the invention, two acids are separately used for two-time acidifying hydrated phospholipid and non-hydrated phospholipid in crude oil, so that the phospholipid content in the fourth-grade rapeseed oil is reduced to meet the qualified requirement of a heating test, and therefore, the problem that four-grade rapeseed oil produced from imported rapeseed oil cannot meet the quality standards in a heating test is solved.

A method for producing diesel grade fuel of plant origin by transesterifying a refined vegetable oil with a charge of a C1-C4 alcohol in the presence of a catalyst and at least 0.2 parts by volume, related to unit volume of refined vegetable oil, of an aliphatic hydrocarbon solvent with a boiling point of -42 DEG C to 200 DEG C, comprises mixing the oil, alcohol, catalyst and solvent in a single reaction vessel under homogeneous conditions which promote transesterif ication to 95-98% completion and which suppress reverse glycerolysis, without stopping transesterif ication to remove by- product polar glycerol, and without subjecting the oil/fuel mixture to a further transesterif ication step with a fresh charge of alcohol and catalyst.

The invention provides a method for preparing peanut oil by baking and squeezing at low temperature. The method comprises the following steps: screening, baking at low temperature, squeezing at low temperature, purifying and the like. Through reasonable screening, and baking for deoiling, the quality safety of the peanut oil is improved, and the prepared peanut oil is nutritional, healthy, and better and stronger in fragrance; through strict control over the process temperature, the influence on the heat-sensitive substances in peanuts is relatively small, and the flavor and the nutrition of the peanuts are effectively retained.

The invention discloses a functional microbial transformation method with combination of permeabilization and ultrasound. The method comprises the following steps of: permeabilizing microbial cells by a Decon solution to reduce the content of phospholipids in cell walls and cytoplasmic membranes; adding calcium ions to a system during the permeabilization so as to facilitate the sedimentation and the transportation of exogenous DNA (Deoxyribonucleic Acid); and then exerting low-frequency ultrasonic waves of 20-80 kHz to transport the DNA and ribonucleic acid (RNA) to be transformed into the cells. By virtue of the Decon permeabilization, the power of the ultrasound in the ultrasonic transformation can be relatively reduced, the ultrasound time is short, and the damage of the ultrasound to the cells is alleviated; by reasonably choosing a permeabilization reagent and determining corresponding concentration, a medium solution simultaneously meeting the requirements of the permeabilization and the ultrasonic transformation is obtained; and exogenous nucleic acid molecule transformation of host bacteria in a larger range can be realized, and the transformation efficiency is enhanced, so that an effective technological way for genetic engineering transformation and new functional microbial culture is provided, the efficiency is enhanced, and the cost and the operation difficulty are reduced.

The invention discloses a surface-modified salbutamol suspension type non-Freon inhalation aerosol and a preparation method thereof. The surface-modified salbutamol suspension type non-Freon inhalation aerosol contains 0.001%-3% of salbutamol with surface modified by using phospholipid by total weight of the aerosol. The surface-modified salbutamol suspension type non-Freon inhalation aerosol hasthe advantages of convenient use, safety and nonirritant because natural nontoxic phospholipid is used as a surface active agent has great low content, and higher clinical application value.

Herein is disclosed a method for producing lipopolysaccharide (LPS), comprising: (a) growing a culture of deep rough mutant bacterial strain in a medium; (b) maintaining the culture in stationary phase for at least about 5 hr; (c) harvesting cells from the culture; and (d) extracting LPS from the cells. The method allows for the production of an LPS which can be used to produce a 3-O-deacylated monophosphoryl lipid A (3D-MLA) having at least about 20 mol % of the hexaacyl congener group. Also herein is disclosed a method of extracting lipopolysaccharide (LPS) from a culture of deep rough mutant bacterial strain cells, comprising: (a) extracting the cells with a solution consisting essentially of at least about 75 wt % of an aliphatic alcohol having from 1 to 4 carbon atoms and the balance water, thereby producing cells with reduced phospholipid content; and (b) extracting the cells with reduced phospholipid content with a solution comprising chloroform and methanol, thereby yielding a solution of LPS in chloroform and methanol (CM). This method provides LPS solutions in CM that have reduced phospholipid content and are produced by relatively simple and inexpensive process steps.

The invention discloses a strong-flavor oil enzymatic-process degumming process, is used for solving the problems of relatively large oil product loss, relatively high water content, relatively long degumming time, non-thorough degumming, relatively high phosphorus content, not easy long-term preservation and the like in the prior art, and is characterized in that trace active phospholipase is added in crude oil for degumming, and the process comprises the following specific process steps: measuring the quantity of oil products in a hydration tank with a ruler, calculating the mass of an enzyme required to be added and the mass of soft water required to be added, measuring the temperature of the crude oil, then adding the soft water and the active phospholipase in a stirring heating-up process, stopping stirring, then naturally settling for 2-4 hours, discharging oil foots, starting up a refrigerating unit for cooling hydration oil, starting to filter oil when the temperature is dropped to about 25 DEG C, and completing the process after the oil filtration is completed. The process has the advantages of advanced process, thorough degumming, resource saving, quality improving and the like, and can be widely applied for degumming in a strong-flavor oil production workshop.

The invention relates to a degumming process of tuna oil, and belongs to the technical field of aquatic product processing. The degumming process comprises the following steps: pretreating tuna crudeoil; adding distilled water into the tuna crude oil after pretreatment, stirring evenly, then adding a compound deglycosylation enzyme, and carrying out preliminary enzymatic hydrolysis, to obtain primary enzymatic hydrolysis fish oil; adding a compound proteinase into the primary enzymatic hydrolysis fish oil, and carrying out enzymatic hydrolysis again, to obtain enzymatic hydrolysis fish oil; heating the enzymatic hydrolysis fish oil, carrying out enzyme inactivation treatment, then cooling to room temperature, centrifuging, and taking an upper floating oily matter, namely the primary degummed fish oil; and adding an acid degumming agent into the primary degummed fish oil, carrying out acid degumming, and then centrifuging to take a supernatant, namely degummed tuna oil. The degumming process has the advantages of mild reaction process, easy control, good degumming effect and high efficiency, and does not destroy the effective nutrient substances in the tuna oil.

The invention relates to a processing method for antioxidant fen-flavor peanut oil and belongs to the technical field of peanut oil finish machining. The processing method comprises the following steps: removing red skins of peanuts, crushing the peanuts, then squeezing peanuts, obtained through microwave treatment, in a low-temperature squeezer to obtain crude oil, and sequentially performing filtering, pickling, low-temperature de-gumming, filtering, vacuum dehydration and anti-oxidation substance adding to obtain antioxidant fen-flavor peanut oil. The peanut oil obtained through the processing method for the antioxidant fen-flavor peanut oil has the advantages that the color is clear, the content of phosphatide is low, and the quality has low probability of changing after long-time storage.

The invention discloses a method for extracting acer truncatum fruit edible oil, and relates to the technical field of vegetable oil processing. The method comprises the steps: shelling the acer truncatum fruits to obtain acer truncatum seeds, putting the acer truncatum seeds into a low-temperature freezer, carrying out freezing treatment for 30-60 minutes at the temperature from -130 DEG C to -160 DEG C, then, carrying out unfreezing for 2 hours at normal temperature, carrying out drying, then carrying out crushing to 30 meshes, then carrying out enzyme deactivation and enzymolysis, carryingout high-speed centrifugation for 15 minutes at a rotating speed of 9800 r/min, and carrying out separation to obtain the acer truncatum edible oil. The extraction method disclosed by the invention isenvironment-friendly, pollution-free, short in time and high in crude oil quality; the oil extraction process is simple and convenient to operate, oil extraction conditions are mild, protein cannot be damaged, the acid value, the phospholipid content and the peroxide value of the extracted oil product are low, a water layer and residues obtained after centrifugal separation contain recyclable substances such as soluble polysaccharide, protein and tea saponin, and compared with a traditional process, the method reduces sewage and waste.