Method for extracting tea seed oil by using enzymatic hydrolysis method

A technology of tea seed oil and tea seed, which is applied in the direction of fat oil/fat production and fat generation, can solve the problems of no patent application in applied research, low use of chemical reagents, limited basic research, etc., and achieve simple equipment and refined oil Good quality and safe operation

Active Publication Date: 2013-08-14
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the production of oils and fats, it can be seen that improper physical means such as high temperature are used to increase the oil yield, which will lead to changes in the properties of oils and fats, which will cause great harm to human health. It has been frequently reported
The aqueous enzymatic method has mild conditions, high oil quality, low energy consumption, and low chemical reagent usage. It is at the advanced level of oil extraction technology in the world. However, it has not been popularized in my country, mainly because basic research is relatively limited. Applied research has not yet seen patent applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] After drying the tea seeds, shelling and removing the clothes, use a blender to grind the tea seeds into powder, take 5g of tea seed powder, add water and mix according to the ratio of material to liquid (1:4 (g:mL), then put in 100 Inactivate the enzyme in a water bath for 5 minutes at ℃, cool to 45℃, and add a concentration of 5.0×10 under the condition of natural pH -4 g / mL neutral protease dilution (the amount of neutral protease used is 150U) for enzymatic hydrolysis for 2 hours, stirring constantly. Then put it into a water bath at 100°C for a second enzyme inactivation, cool to room temperature and centrifuge for 40 minutes. The oil layer was extracted by adding 30-50 mL of anhydrous ether, and the solvent was rotary evaporated to obtain an oil sample, and the oil yield was 22.98%.

Embodiment 2

[0015] After drying the tea seeds, shelling and removing the clothes, use a blender to grind the tea seeds into powder, take 5g of tea seed powder, add water and mix according to the ratio of material to liquid (1:4 (g:mL), then put in 100 Inactivate the enzyme in a water bath for 5 minutes at ℃, cool to 45℃, and add a concentration of 5.0×10 under the condition of natural pH -4 g / mL pectinase dilution (the amount of pectinase used is 100U) for enzymolysis for 2 hours, stirring constantly. Then put it into a water bath at 100°C for a second enzyme inactivation, cool to room temperature and centrifuge for 40 minutes. The oil layer was extracted by adding 30-50 mL of anhydrous ether, and the solvent was rotary evaporated to obtain an oil sample, and the oil yield was 22.94%.

Embodiment 3

[0017] After drying the tea seeds, shelling and removing the clothes, use a blender to grind the tea seeds into powder, take 5g of tea seed powder, add water and mix according to the ratio of material to liquid (1:4 (g:mL), then put in 100 Inactivate the enzyme in a water bath at ℃ for 5 minutes, cool to 55℃, and add a concentration of 1.0×10 under the condition of natural pH -3 g / mL cellulase dilution (the amount of cellulase used is 300U) for enzymolysis for 2 hours, stirring constantly. Then put it into a water bath at 100°C for a second enzyme inactivation, cool to room temperature and centrifuge for 40 minutes. The oil layer was extracted by adding 30-50 mL of anhydrous ether, and the solvent was rotary evaporated to obtain an oil sample, and the oil yield was 20.59%.

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PUM

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Abstract

The invention discloses a method for extracting tea seed oil by using an enzymatic hydrolysis method, which comprises the following steps of: airing, unshelling, husking and pulverizing tea seeds; carrying out enzymolysis on the tea seeds in the material-liquid ratios of 1:3 to 1:5 at the extraction temperature of 40-60 DEG C for 1-5h with the enzyme quantity of 40-600U under the pH natural condition; centrifugally layering; adding an organic solvent into centrifugal liquid and extracting; and rotating and evaporating to obtain an oil sample. By adopting the scheme, the method has the advantages of low temperature for equipment operation, low energy consumption, high purity of extracted oil, low content of phospholipid, low acid value and low peroxide value, shallow color, low BOD (Biochemical Oxygen Demand) and COD (Chemical Oxygen Demand) values, easiness of treatment and less pollution, and the oil yield ratio is up to 23.1 percent.

Description

technical field [0001] The invention relates to a method for extracting tea seed oil from tea seeds. Background technique [0002] my country is a big tea country, and tea seeds are by-products of tea production, which are similar to camellia oleifera seeds and camellia seeds, and have high oil content. The physical and chemical properties of tea seed oil are very similar to those of camellia oleifera oil and olive oil. The unsaturated fatty acids in the molecule are mainly oleic acid and linoleic acid, and the content of oleic acid is between 40% and 57%, which has higher antioxidant capacity. Sex and better physiological activity, it is considered to be a kind of fatty acid beneficial to health. In addition to a large amount of unsaturated fatty acids, tea seed oil also contains a certain amount of tea polyphenols, squalene, tocopherol, vitamin E and other important health ingredients. Tea seed oil has the functions of clearing away heat and detoxification, astringent an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/04C11B1/10
Inventor 王晓琴黄东方伍华告
Owner HUAQIAO UNIVERSITY
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