Preparation method of zein-bee pollen extract microcapsules
A technology of zein and bee pollen, which is applied in the field of food processing, can solve the problems of uneven encapsulation rate of microcapsule particles and the inability to release active ingredients in the stomach and intestines, and achieves the improvement of Fe2+ complexing ability and iron reduction resistance. Oxidative power, the effect of avoiding denaturation
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Embodiment 1
[0027] A preparation method of a zein-bee pollen extract microcapsule disclosed by the present invention specifically comprises the following steps:
[0028] (1) Preparation of bee pollen extract
[0029] Mix bee pollen and 70% ethanol solution according to the solid-liquid ratio (w / v) of 1:20, heat and reflux at 70°C for 2h, centrifuge at 4000r / min for 10min, and take the supernatant; After two refluxes, the supernatant was obtained by centrifugation after each reflux, the supernatants after each reflux and centrifugation were mixed together, concentrated by rotary evaporation at 40° C. to obtain a bee pollen extract.
[0030] (2) Preparation of zein-bee pollen extract microcapsules
[0031] a. With zein as the shell and bee pollen extract as the core. According to the mass ratio of zein and bee pollen extract is 2:1, weigh zein and bee pollen extract for later use; then dissolve zein into 80% ethanol solution to prepare 8% (w / v) Add the weighed bee pollen extract to the ...
Embodiment 2
[0038] 1. The zein-bee pollen extract microcapsules prepared in Example 1 and the comparative example were carried out to measure relevant parameters, and the determination methods all adopted spectrophotometry.
[0039] (1) Determination of flavonoid content in zein-bee pollen extract microcapsules
[0040] a. Rutin standard curve drawing
[0041] Weigh 0.01g of rutin and dissolve it in 85% (v / v) methanol, dilute to 50mL, and prepare 0.2mg / mL rutin standard solution. Add 0, 0.4, 0.8, 1.2, 1.6, 2.0mL rutin standard solution into 10mL test tubes respectively. Add 0.4mL 5% NaNO 3 , shake well and stand for 6min, add 0.4mL 10% Al(NO 3 ) 3 , oscillated and mixed, left for 6min, added 4mL of 4% NaOH, and then dilute to 10mL with methanol, mixed evenly, left for 15min, and measured the absorbance value at 510nm. Draw the standard curve, the measured curve equation is y=9.8298x+0.0223, R 2 = 0.9968.
[0042] b. Determination of flavonoid content in microcapsules
[0043] Weig...
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