Method for extracting grease from oil-tea camellia seed kernel and recovering saponin and saccharide active substances

An active substance and oil extraction technology, applied in the application field of biotechnology in grain, oil and food processing, to achieve the effect of mild action conditions, high yield of clear oil, and high recovery rate of saponins

Active Publication Date: 2010-12-08
ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a method for synchronously extracting oil and recovering active substances such as saponins and sugars from camellia oleifera seeds. When extracting oil from seeds, there are difficulties and cumbersomeness in the recovery and utilization of by-products, and it is necessary to break through the technical limitations and existing problems in the above patents

Method used

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  • Method for extracting grease from oil-tea camellia seed kernel and recovering saponin and saccharide active substances
  • Method for extracting grease from oil-tea camellia seed kernel and recovering saponin and saccharide active substances
  • Method for extracting grease from oil-tea camellia seed kernel and recovering saponin and saccharide active substances

Examples

Experimental program
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Effect test

Embodiment 1

[0034]Take 1 kg of shelled Camellia oleifera seeds, grind them coarsely with a pulverizer, then finely pulverize them with a grinder, add 5L of water, stir evenly, adjust the pH of the system to 5.5, the temperature is 40°C, and add 0.5% pectinase by weight of Camellia oleifera seeds , after 4 hours of enzymolysis with slow stirring, the temperature of the system was raised to 80°C and kept for 10 minutes, and then centrifuged at 3500rpm for 10 minutes with a floor-standing centrifuge. The enzymolysis liquid was divided into three layers: clear oil, liquid phase and residue. Take out clear oil and weigh 403g, and the clear oil yield is 87.4%. After the liquid phase is concentrated by multi-effect concentration equipment, it is spray-dried to obtain active substances containing saponins and sugars, and the calculated yield of saponins is 67.8%. The quality indicators of each product are shown in Table 1-3.

[0035] Table 1 The physical and chemical properties of the Camellia o...

Embodiment 2

[0043] Take 500g of shelled Camellia oleifera seeds, pulverize them on a pulverizer, then pulverize them finely in a grinder, add 3L of water, stir well, adjust the pH of the system to 6.5, the temperature is 60°C, and add 1.5% pectinase by weight of Camellia oleifera seeds , after slow stirring for 3 hours of enzymolysis, the temperature of the system was raised to 85°C and kept for 8 minutes to inactivate the enzyme, and then centrifuged at 4000rpm for 15 minutes in a floor-standing centrifuge. The enzymolysis solution was divided into three layers: clear oil, liquid phase and residue; clear oil The yield is 89.4%. After the liquid phase is concentrated by multi-effect concentration equipment, it is spray-dried to obtain active substances containing saponins and sugars, and the calculated yield of saponins is 70.1%. The quality indicators of each product are shown in Table 1-3.

Embodiment 3

[0045] Take 1500g of shelled Camellia oleifera seeds, pulverize them on a pulverizer and then pulverize them finely with a grinder, add 6L of water, stir well, adjust the pH of the system to 8.0, the temperature is 50°C, and add 1% pectin by weight of Camellia oleifera seeds Enzyme, after slow stirring for 2 hours, the temperature of the system was raised to 90°C and kept for 5 minutes to inactivate the enzyme, and then centrifuged at 3000rpm for 15 minutes in a floor-standing centrifuge. The enzymolysis solution was divided into three layers: clear oil, liquid phase and residue; The clear oil yield is 89%. After the liquid phase is concentrated by multi-effect concentration equipment, it is spray-dried to obtain active substances containing saponins and sugars, and the calculated yield of saponins is 71.9%.

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Abstract

The invention discloses a method for extracting grease from oil-tea camellia seed kernel and recovering saponin and saccharide active substances, comprising the following steps of: (1) crushing and tempering: with hull-removed oil-tea camellia seed kernels as raw materials, crushing and grinding the materials, adding water for uniformly stirring, wherein the quality-volume ratio of the oil-tea camellia seed kernels to the water is 1kg:2-8L and controlling the temperature of the system to be 30-60DEG C; (2) carrying out the enzymolysis: adjusting the pH value of the system to be 3.5-8.0, adding pectinase the weight of which is 0.1-2 percent that of the camellia seed kernels and carrying out the enzymolysis for 2-5h; (3) heating and centrifuging: treating the system at the temperature of 80-90DEG C for 5-15min after enzymolysis; and (4) recovering the grease and the active substances: in which, the centrifuged and separated system comprises boiled oil, liquid phase and residue, taking out the boiled oil and spray-drying the liquid phase to obtain the active substances containing the saponin and saccharide. The method for extracting the grease avoids the high-temperature pretreatment of high-temperature enzymolysis of oil materials and can be used for synchronously recovering oil and a saponin and saccharide mixture which can be used as a saponin crude product or a feed additive.

Description

technical field [0001] The invention relates to a process technology for synchronously extracting oil, saponin and sugar biologically active substances from camellia oleifera seed kernels by using an aqueous enzymatic method, and belongs to the application technical field of biotechnology in grain, oil and food processing. Background technique [0002] Camellia oleifera is an excellent native tree species unique to my country. Together with olive, oil palm and coconut, it is listed as the world's four largest woody oil plants. The annual output of camellia oleifera seeds in my country is about 1.06 million tons, and the annual output of camellia oleifera seed oil is 277,000 tons. Camellia oleifera seed contains 66%-72% kernel, 40%-60% oil in the kernel, about 10% protein, 8% saponin, and about 25% sugar. The fatty acid composition of Camellia oleifera seed oil is similar to olive oil known as the "Queen of Vegetable Oils". The unsaturated fatty acid content is as high as m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/04C12S3/18C08B37/00C07J63/00C07H15/256C07H1/08
Inventor 王瑛瑶栾霞马榕李爱科刘倩茹
Owner ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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