Extraction method of effective components of Chinese angelica

Abstract

The invention relates to an extraction method of effective components of Chinese angelica. The extraction method comprises the following steps: 1, Chinese angelica is crushed; 2, crushed Chinese angelica is added to a solvent A solution with the pH value of 5.5; 3, cellulase with the concentration of 0.3mg.mL<-1> is added to the solution obtained in step 2; 4, the solution obtained in step 3 is subjected to enzymatic hydrolysis for 6h at a constant temperature of 55DEG C; 5, isoamylase with the concentration of 0.3mg.ml<-1> is added to the solution obtained in step 4; 6, a solution obtained in step 5 is subjected to enzymatic hydrolysis for 6h at a constant temperature of 55DEG C; 7, the solution obtained in step 6 is subjected to reduced pressure filtration after completing the enzymatic hydrolysis, and the obtained filtrate is preserved in a dark place; 8, the filter residue obtained by filtering in step 7 is used for extracting ligustilide, an eight time solvent B is added each time, reflux extraction is twice carried out for 2h, the obtained first filtrate and the obtained second filtrate are mixed to obtain a mixed filtrate which is a ligustilide solution, and the ligustilide solution is preserved in a low temperature dark place; 9, the filter residue obtained in step 8 is used for extracting angelica polysaccharides, an fifteen time solvent C is added to the filter residue to extract; 10, the filtrate obtained in step 9 is concentrated to obtain a concentrate; and 11, a solvent D is added to the concentrate obtained in step 10 until that the concentration of the solvent D reaches 75% to obtain a deposition, and the deposition is centrifuged by a centrifuge to obtain the angelica polysaccharides.

Description

technical field [0001] The invention belongs to the extraction process of effective components of traditional Chinese medicine. Background technique [0002] The medicinal material Angelica sinensis is the root of the perennial herb of Umbelliferae, and it is a famous traditional Chinese medicine. [0003] The current extraction process from Angelica sinensis mainly includes the above three main active ingredients, namely: fat-soluble ingredients represented by ligustilide and water-soluble ingredients represented by ferulic acid and Angelica polysaccharide. Relevant studies have confirmed that the fat-soluble components of Angelica sinensis represented by ligustilide and the water-soluble components of Angelica sinensis represented by ferulic acid are unstable, and are prone to isomerization reactions such as dehydrogenation, oxidation, hydrolysis, and degradation. The conditions for extraction, separation, purification and storage are relatively harsh. [0004] The extra...

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Problems solved by technology

These traditional methods have gone through a long history. Due to their reasonable and applicable scientific and technological core, they are still commonly used methods for extracting Angelica sinensis. However, these methods have obvious deficiencies and problems. Low-polarity compounds (including volatile oil) are the important material basis for angelica to exert some of its medicinal effects. However, steam distillation is traditionally used for extraction. Such high temperature is not conducive to the extraction of unstable low-polarity compounds. In addition, the efficacy of traditional Chinese medicine It does not come from a single active chemical component, but from the synergistic effect between multiple active components, even related to some inactive components, but traditional extraction processes mostly target a certain (or a certain type) component, And use the extraction amount of a certain component (or a certain type) component as an index to evaluate different extraction methods, so the utilization rate of medicinal materials is not high, and the yield of active ingredients is low

Methods such as supercritical extraction, ultrasonic and microwave-assisted extraction belong to the application of new technologies in the extraction of medicinal materials. It will take a certain amount of time to realize the large-scale production of pharmaceuticals. Although the damage to active ingredients by high temperature and high pressure can be avoided, the requirements for equipment are relatively high. Moreover, the initial investment in the production equipment is relatively large, and the maintenance cost is relatively high

In addition, the components with medicinal value in plant traditional Chinese medicine are often some secondary metabolites of the medicinal plant itself, and most of the medicinal plant is the structural material on which it depends for growth and development, such as cellulose, hemicellulose, Lignin and pectin do not have medicinal value. Whether it is the traditional Chinese medicine processing method in our country or the extraction process of modern large enterprises, they all use boiling water or a large amount of organic solvents to try to combine the medicinal components of traditional Chinese medicine with Separation of structural substances that have no medicinal value, the limitation is that the extraction of active ingredients is insufficient or only for one or some ingredients, which reduces the curative effect and wastes a lot of limited traditional Chinese medicine resources

[0008] Although the existing technology can satisfy the separation of active ingredients of Angelica sinensis in the processing of traditional Chinese medicinal materials in existing enterprises, the existing technology is all aimed at certain medicinal ingredients, such as: extracting ferulic acid, whether it is heated distillation or Organic solvent extraction, all can cause the loss to ligustilide, promptly adopt existing report process to extract ligustilide after ferulic acid can not be extracted again; , the remaining residues cannot be used for the extraction of ferulic acid

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