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Method for extracting EGCG (epigallocatechin gallate) in tea leaves by water extraction

A tea and water extraction technology, applied in the field of plant extraction, can solve the problems of high extraction temperature, large liquid-to-solid ratio, environmental disasters, etc., and achieve the effects of simple operation, reduced emissions, and low cost

Inactive Publication Date: 2014-04-30
广东省农业科学院饮用植物研究所
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  • Application Information

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Problems solved by technology

[0003] The extraction solvent of EGCG in tea is mainly water or a certain concentration of ethanol. The extraction temperature is high, the cycle is long, the ratio of liquid to material is large, and a large amount of organic solvents are used to bring great safety hazards and environmental disasters to production and human beings.

Method used

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  • Method for extracting EGCG (epigallocatechin gallate) in tea leaves by water extraction
  • Method for extracting EGCG (epigallocatechin gallate) in tea leaves by water extraction
  • Method for extracting EGCG (epigallocatechin gallate) in tea leaves by water extraction

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Embodiment 1

[0028] 1. Sample preparation

[0029] Jinxuan summer green tea: purchased from Minghuang Tea Co., Ltd. The green tea is pulverized and passed through a 100-mesh sieve as a sample.

[0030] 2. Preparation of EGCG extract and calculation of yield

[0031] Preparation of EGCG extract: Weigh 3g sample, add a certain amount of deionized water, extract under certain conditions, filter the extracted solution, then centrifuge at 80,000rpm for 10min, recover the supernatant, constant volume, and finally use high-efficiency liquid The content of EGCG in the extract was determined by phase chromatography.

[0032] EGCG yield calculation:

[0033] EGCG yield (%) = [measured EGCG weight / sample weight] × 100%

[0034] 3. Experimental Design and Statistical Analysis

[0035] 1) Single factor experiment

[0036] Weigh 3g of sample in a beaker, extract it with deionized water, carry out single factor experiments in a constant temperature water bath according to different extracti...

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Abstract

The invention discloses a method for extracting EGCG (epigallocatechin gallate) in tea leaves by water extraction; according to the method, the GCG in the tea leaves is extracted with de-ionized water, and on the basis of a single factor experiment, the extraction condition of the EGCG is optimized by using a response surface method, so as to achieve the optimal extraction condition that the pH (potential of hydrogen) of the de-ionized water is adjusted to be 6, the liquid-material ratio is 41, namely 41ml of de-ionized water is added in per gram of green tea, the mixture is extracted for 34min in a constant-temperature thermostat water bath of 85 DEG C and then is filtered, so as to obtain an EGCG extract, the extraction is repeated for once, and then the EGCG content is detected, wherein the yield of the EGCG can reach 3.40%.

Description

technical field [0001] The invention relates to a method for water extraction of EGCG in tea leaves, which belongs to the technical field of plant extraction, and in particular relates to a method for optimizing tea EGCG extraction conditions by using a response surface method. . Background technique [0002] Catechin in tea is an important class of natural active substances in tea. It is a natural antioxidant and free radical scavenger. It is widely used in food processing, medicine and health care and other fields. Catechin accounts for 60% to 80% of the total tea polyphenols in tea, mainly including epigallocatechin gallate (EGCG), epicatechin gallate (ECG), epigallocatechin gallate (EGC ) and epicatechin (EC), among which EGCG has the highest content, accounting for about 50% to 60% of catechin. A large number of experiments have proved that EGCG has a variety of physiological activities. EGCG has the strongest antioxidant effect among catechins. With the in-depth und...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23F3/18
Inventor 潘顺顺孙世利凌彩金操君喜苗爱清张婷
Owner 广东省农业科学院饮用植物研究所
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