Soothing and sunscreen spray as well as preparation method and application thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to soothing and sunscreen spray as well as a preparation method and application thereof. The spray is prepared from the following raw materials: cyclopentasiloxane, octyl polymethylsiloxane, polydimethylsiloxane, butanediol, polymethylsilsesquioxane, ethylhexyl salicylate, diethylaminohydroxybenzoyl hexyl benzoate, octocrylene, lauryl polyethylene glycol (PEG)-10 tri (trimethylsiloxy) silyl ethyl polydimethylsiloxane, ethylhexyl triazinone, an organic solvent, an organic solvent and a solvent. The composition is prepared from herba centellae extract, radix gentianae extract and water. The spray prepared by the invention has excellent effects of soothing the skin, blocking the sun and preventing the skin from dullness.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a soothing and sunscreen spray and a preparation method and application thereof. Background Art

[0002] The main function of sunscreen cosmetics is to block ultraviolet rays and protect the skin from ultraviolet damage. Ultraviolet rays can cause various damages to the skin, including tanning, sunburn and aging. Sunscreen cosmetics protect the skin from these damages by absorbing, reflecting or scattering ultraviolet rays, reducing the direct exposure of ultraviolet rays to the skin.

[0003] The ingredients of sunscreen cosmetics mainly include chemical sunscreens and physical sunscreens. Chemical sunscreens protect the skin by absorbing ultraviolet rays and converting them into heat, while physical sunscreens protect the skin by reflecting and scattering ultraviolet rays. In addition, some sunscreen products also contain antioxidants and anti-inflammatory ingredients that help reduce inflammatory reactions and pigmentation caused by ultraviolet rays.

[0004] Different types of sunscreen cosmetics are suitable for different skin types and usage scenarios. Chemical sunscreens are generally suitable for use in environments with strong ultraviolet rays, while physical sunscreens are suitable for sensitive skin due to their safety and stability.

[0005] At the same time, since sunscreen cosmetics use a variety of chemical ingredients, they are prone to skin allergies or discomfort; at the same time, the sunscreen strength varies. Therefore, providing a soothing and sunscreen spray is a technical problem that needs to be solved at present. Summary of the invention

[0006] In view of the above technical problems, the present invention proposes a technical solution of the present invention, which includes a soothing and sunscreen spray and a preparation method and application thereof.

[0007] On the one hand, the present invention provides a soothing and sunscreen spray, the raw materials of which include cyclopentasiloxane, octyl polymethicone, polydimethylsiloxane, butylene glycol, polymethylsilsesquioxane, ethylhexyl salicylate, diethylaminohydroxybenzoyl hexyl benzoate, octocrylene, lauryl PEG-10 tris(trimethylsiloxy)silylethyl polydimethicone, ethylhexyl triazone and water.

[0008] Preferably, the soothing and sunscreen spray comprises, in terms of mass percentage, the following raw materials: 1-5% cyclopentasiloxane, 0.5-3% octyl polymethicone, 0.5-2% polydimethylsiloxane, 8-12% butylene glycol, 0.5-2% polymethylsilsesquioxane, 0.4-1.5% ethylhexyl salicylate, 0.3-1.5% diethylaminohydroxybenzoyl hexyl benzoate, 0.3-1.5% octocrylene, 4-8% lauryl PEG-10 tris(trimethylsiloxy)silylethyl polydimethicone, 0.4-1.5% ethylhexyl triazone and the remainder water.

[0009] Preferably, in the soothing and sunscreen spray, the mass ratio of butylene glycol to lauryl PEG-10 tris(trimethylsiloxy)silylethyl polydimethicone is 1.5-2.2:1.

[0010] Preferably, the soothing and sunscreen spray further comprises 0.5-2% of Centella asiatica extract and 0.5-1.8% of Gentiana dahurica extract.

[0011] Preferably, the Centella asiatica extract suitable for the soothing and sunscreen spray of the present invention needs to be an extract prepared by a process including enzymatic hydrolysis of Centella asiatica with bromelain and fermentation by fermentation bacteria. The extract prepared by this process has a better effect than the Centella asiatica extract prepared by traditional alcohol extraction, water extraction or extraction process, and can better exert the efficacy of the active ingredients of Centella asiatica.

[0012] Preferably, the gentian extract suitable for the soothing and sunscreen spray of the present invention is an extract prepared by extracting the gentian root with a 40-55% ethanol solution and ultrafiltration. This process can effectively promote the extraction and performance of the active ingredients of the gentian root.

[0013] Preferably, the mass ratio of the Centella asiatica extract to the Gentiana dahurica extract is 0.8-1:1.

[0014] Preferably, the preparation method of the Centella asiatica extract comprises:

[0015] Step 1: Crush the dried whole herb of Centella asiatica, add water, heat and keep warm to obtain product 1;

[0016] Step 2: sterilize product 1, add bromelain, glucose, dipotassium hydrogen phosphate and fermentation bacteria, ferment and sterilize to obtain product 2;

[0017] Step 3: Filter the product 2 to obtain a filtrate, and dry it to obtain the Centella asiatica extract.

[0018] Preferably, the mass of added water is 10-30 times that of the whole herb of Centella asiatica.

[0019] Preferably, the heating and heat preservation conditions are: heating to 60-80° C. and heat preservation for 2-10 hours.

[0020] Preferably, the amount of bromelain added is 600-1500 U / g product 1.

[0021] Preferably, the amount of glucose added is 1-4% by mass of the product 1.

[0022] Preferably, the amount of dipotassium hydrogen phosphate added is 0.02-0.1% of the mass of the product 1.

[0023] Preferably, the fermentation bacteria are Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322 in a quantitative ratio of 1:0.4-0.8.

[0024] Preferably, the fermentation bacteria dosage is 1×10 9 -9×10 9 cfu / g product 1.

[0025] Preferably, the fermentation temperature is 30-45°C and the fermentation time is 65-150 hours.

[0026] Preferably, the preparation method of the gentian extract comprises:

[0027] (1) Crush the dried gentian root, add 8-40 times the mass of ethanol aqueous solution of the gentian root, heat at 50-65° C. and stir for 3-10 hours to obtain an intermediate product 1;

[0028] (2) The intermediate product 1 is filtered, ultrafiltered, and then concentrated under reduced pressure to 0.05-0.12 times the mass of the intermediate product, and dried to obtain the gentian extract.

[0029] Preferably, the mass concentration of the ethanol aqueous solution is 40-55%.

[0030] Preferably, the ultrafiltration is performed using an ultrafiltration membrane with a molecular weight cut-off lower than 800 Da.

[0031] On the other hand, the present invention also provides a preparation method of the soothing and sunscreen spray, comprising the following steps: adding cyclopentasiloxane, octyl polymethicone, polydimethylsiloxane, butylene glycol, polymethylsilsesquioxane, ethylhexyl salicylate, diethylaminohydroxybenzoyl hexyl benzoate, octocrylene, lauryl PEG-10 tris(trimethylsiloxy)silylethyl polydimethicone and ethylhexyl triazone to a container, heating to 60-85° C., stirring and mixing; then adding water and stirring; cooling, then adding Centella asiatica extract and gentiana extract and stirring and mixing, and sterilizing to obtain the soothing and sunscreen spray.

[0032] Preferably, the above spray can be added to a spray container to make a finished cosmetic product, or a propellant can be added as needed to obtain a spray product.

[0033] Another aspect of the present invention provides the use of the soothing, sunscreen spray in non-therapeutic cosmetics.

[0034] Beneficial effects of the present invention:

[0035] The present invention adopts cyclopentasiloxane, octyl polymethicone, polydimethylsiloxane and polymethylsilsesquioxane as emollient ingredients, and is matched with ethylhexyl salicylate, diethylaminohydroxybenzoyl hexyl benzoate and octocrylene as sunscreen ingredients, so as to give the spray excellent skin moisturizing and sunscreen effects. Meanwhile, lauryl PEG-10 tris(trimethylsiloxy)silylethyl polydimethicone and butylene glycol are added, and the ratio of the two is controlled to give the spray better stability and use effect. Meanwhile, active ingredients of Centella asiatica and gentian root which can soothe and whiten the skin are added to give the spray the effect of soothing the skin and preventing the skin from becoming dull, and the extraction process of the extract is further optimized to further enhance the anti-inflammatory effect of the spray.

[0036] The spray prepared by the invention has excellent effects of soothing the skin, protecting the skin from the sun and preventing the skin from becoming dull.

[0037] The Centella asiatica extract and the gentian extract prepared by the present invention have excellent effects of reducing the expression of IL-6 and TNF-α, can effectively inhibit inflammation, and have a good skin soothing effect.

[0038] The invention adds bromelain during the fermentation process of the Centella asiatica, which can effectively promote the increase of the amount of IL-6 and TNF-α expression inhibited by the extract. When fermenting, the fermentation bacteria use the combination of Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322 to effectively improve the effect of inhibiting inflammation. The combination of the two can synergistically enhance the effect of inhibiting the expression of IL-6 and TNF-α. The co-fermentation of the two can promote the richness of the soothing active ingredients and the soothing effect of the extract can be effectively improved after concentration and other treatments in the later stage; and when the ratio of the two is 1:0.4-0.8, the obtained effect is better.

[0039] When extracting the gentian root of the present invention, 40-55% ethanol aqueous solution is used for extraction and ultrafiltration membrane with a molecular weight cutoff lower than 800Da is used for interception treatment, which can effectively enhance the soothing effect of the extract and give the extract better use efficiency.

[0040] When the Centella asiatica extract and the gentian extract of the present invention are combined, a mutually promoting relationship is generated within a certain ratio range, and an antagonistic effect is generated within other ratio ranges. When a combination with excellent soothing performance is selected, the effect is optimal when the mass ratio of the Centella asiatica extract and the gentian extract is 0.8-1:1.

[0041] The sunscreen spray prepared by the present invention has an SPF value of 47.6, a refreshing and non-greasy score of 9.6, and is placed at 55°C for one week without stratification and precipitation. The spray of the present invention has an excellent sunscreen effect, is refreshing and non-greasy after application, and has good temperature resistance and stability.

[0042] In the preparation system of the present invention, when the usage ratio of butylene glycol to PEG-10 tris(trimethylsiloxy)silylethyl polydimethylsiloxane is within the range of 1.5-2.2:1, the product effect is better.

[0043] The spray preparation method of the invention is simple, the source of raw materials is convenient, and the spray effect is excellent, and has good application prospects. DETAILED DESCRIPTION

[0044] The present invention will be described below in conjunction with specific embodiments, and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments are used to illustrate the present invention, rather than to limit the present invention.

[0045] Lactobacillus mucilaginosus CICC 6122 and Lactobacillus plantarum CICC 20322 were purchased from China Industrial Microbiological Culture Collection Administration Center. Cyclopentasiloxane: Jinan Yunuo Chemical Co., Ltd. Octyl polymethylsiloxane: Shandong Suihua Biotechnology Co., Ltd. Polydimethylsiloxane: Hubei Xinghengye Technology Co., Ltd. Polymethylsilsesquioxane: Shanghai Myrel Biochemical Technology Co., Ltd. Octocrylene: Shandong Maoyuan Chemical Co., Ltd.

[0046] 1. Preparation and testing of extracts

[0047] A. Preparation of Centella asiatica extract

[0048] Centella Asiatica Extract 1:

[0049] The preparation method is as follows: Step 1: Grind the dried whole herb of Centella asiatica to a particle size of less than 80 meshes, add 18 times the mass of the whole herb of Centella asiatica, heat to 75°C, keep warm and stir at 60 rpm for 8.5 hours to obtain product 1;

[0050] Step 2: sterilize product 1, cool to room temperature, add bromelain, glucose, dipotassium hydrogen phosphate and fermentation bacteria, perform anaerobically fermentation at 35°C for 135 hours, sterilize, and obtain product 2;

[0051] Step 3: Filter the product 2 using a filter membrane with a pore size of 0.2 μm to obtain a filtrate, and freeze-dry at -40°C to a water content of 2.67 wt%, to obtain the Centella asiatica extract.

[0052] Among them, the amount of bromelain added is 1200U / g product 1; the amount of glucose added is 3.02% of the mass of product 1; the amount of potassium hydrogen phosphate added is 0.065% of the mass of product 1;

[0053] The fermentation bacteria were Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322 in a ratio of 1:0.6; the fermentation bacteria dosage was 4.80×10 9 cfu / g product 1.

[0054] Centella Asiatica Extract 2:

[0055] The preparation method is as follows: Step 1: grind the dried whole herb of Centella asiatica to a particle size of less than 80 meshes, add 20 times the mass of the whole herb of Centella asiatica, heat to 72°C and keep warm while stirring at 60 rpm for 9 hours to obtain product 1;

[0056] Step 2: sterilize product 1, cool to room temperature, add bromelain, glucose, dipotassium hydrogen phosphate and fermentation bacteria, perform anaerobically fermentation at 36°C for 120 hours, sterilize, and obtain product 2;

[0057] Step 3: Filter the product 2 using a filter membrane with a pore size of 0.2 μm to obtain a filtrate, and freeze-dry it at -40°C to a water content of 2.71 wt%, to obtain the Centella asiatica extract.

[0058] Among them, the amount of bromelain added is 1000U / g product 1; the amount of glucose added is 3.28% of the mass of product 1; the amount of potassium hydrogen phosphate added is 0.07% of the mass of product 1;

[0059] The fermentation bacteria were Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322 in a ratio of 1:0.4; the fermentation bacteria dosage was 4.92×10 9 cfu / g product 1.

[0060] Centella Asiatica Extract3:

[0061] The preparation method is as follows: Step 1: Grind the dried whole herb of Centella asiatica to a particle size of less than 80 meshes, add 18 times the mass of the whole herb of Centella asiatica, heat to 75°C, keep warm and stir at 60 rpm for 8.5 hours to obtain product 1;

[0062] Step 2: sterilize product 1, cool to room temperature, add glucose, dipotassium hydrogen phosphate and fermentation bacteria, perform anaerobically fermentation at 35°C for 135 hours, sterilize, and obtain product 2;

[0063] Step 3: Filter the product 2 using a filter membrane with a pore size of 0.2 μm to obtain a filtrate, and freeze-dry at -40°C to a water content of 2.67 wt%, to obtain the Centella asiatica extract.

[0064] Among them, the amount of glucose added is 3.02% of the mass of product 1; the amount of potassium hydrogen phosphate added is 0.065% of the mass of product 1;

[0065] The fermentation bacteria were Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322 in a ratio of 1:0.6; the fermentation bacteria dosage was 4.80×10 9 cfu / g product 1.

[0066] Centella Asiatica Extract4:

[0067] The preparation method is as follows: Step 1: Grind the dried whole herb of Centella asiatica to a particle size of less than 80 meshes, add 18 times the mass of the whole herb of Centella asiatica, heat to 75°C, keep warm and stir at 60 rpm for 8.5 hours to obtain product 1;

[0068] Step 2: sterilize product 1, cool to room temperature, add bromelain, glucose, dipotassium hydrogen phosphate and fermentation bacteria, perform anaerobically fermentation at 35°C for 135 hours, sterilize, and obtain product 2;

[0069] Step 3: Filter the product 2 using a filter membrane with a pore size of 0.2 μm to obtain a filtrate, and freeze-dry at -40°C to a water content of 2.67 wt%, to obtain the Centella asiatica extract.

[0070] Among them, the amount of bromelain added is 1200U / g product 1; the amount of glucose added is 3.02% of the mass of product 1; the amount of potassium hydrogen phosphate added is 0.065% of the mass of product 1;

[0071] The fermentation bacteria was Lactobacillus reuteri CI CC 6122; the fermentation bacteria dosage was 4.80×10 9 cfu / g product 1.

[0072] Centella Asiatica Extract 5:

[0073] The preparation method is as follows: Step 1: Grind the dried whole herb of Centella asiatica to a particle size of less than 80 meshes, add 18 times the mass of the whole herb of Centella asiatica, heat to 75°C, keep warm and stir at 60 rpm for 8.5 hours to obtain product 1;

[0074] Step 2: sterilize product 1, cool to room temperature, add bromelain, glucose, dipotassium hydrogen phosphate and fermentation bacteria, perform anaerobically fermentation at 35°C for 135 hours, sterilize, and obtain product 2;

[0075] Step 3: Filter the product 2 using a filter membrane with a pore size of 0.2 μm to obtain a filtrate, and freeze-dry at -40°C to a water content of 2.67 wt%, to obtain the Centella asiatica extract.

[0076] Among them, the amount of bromelain added is 1200U / g product 1; the amount of glucose added is 3.02% of the mass of product 1; the amount of potassium hydrogen phosphate added is 0.065% of the mass of product 1;

[0077] The fermentation bacteria was Lactobacillus plantarum CI CC 20322; the fermentation bacteria dosage was 4.80×10 9 cfu / g product 1.

[0078] Centella Asiatica Extract6:

[0079] The preparation method is as follows: Step 1: Grind the dried whole herb of Centella asiatica to a particle size of less than 80 meshes, add 18 times the mass of the whole herb of Centella asiatica, heat to 75°C, keep warm and stir at 60 rpm for 8.5 hours to obtain product 1;

[0080] Step 2: sterilize product 1, cool to room temperature, add bromelain, glucose, dipotassium hydrogen phosphate and fermentation bacteria, perform anaerobically fermentation at 35°C for 135 hours, sterilize, and obtain product 2;

[0081] Step 3: Filter the product 2 using a filter membrane with a pore size of 0.2 μm to obtain a filtrate, and freeze-dry at -40°C to a water content of 2.67 wt%, to obtain the Centella asiatica extract.

[0082] Among them, the amount of bromelain added is 1200U / g product 1; the amount of glucose added is 3.02% of the mass of product 1; the amount of potassium hydrogen phosphate added is 0.065% of the mass of product 1;

[0083] The fermentation bacteria were Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322 in a ratio of 1:0.1; the fermentation bacteria dosage was 4.80×10 9 cfu / g product 1.

[0084] Centella Asiatica Extract 7:

[0085] The preparation method is as follows: Step 1: Grind the dried whole herb of Centella asiatica to a particle size of less than 80 meshes, add 18 times the mass of the whole herb of Centella asiatica, heat to 75°C, keep warm and stir at 60 rpm for 8.5 hours to obtain product 1;

[0086] Step 2: sterilize product 1, cool to room temperature, add bromelain, glucose, dipotassium hydrogen phosphate and fermentation bacteria, perform anaerobically fermentation at 35°C for 135 hours, sterilize, and obtain product 2;

[0087] Step 3: Filter the product 2 using a filter membrane with a pore size of 0.2 μm to obtain a filtrate, and freeze-dry at -40°C to a water content of 2.67 wt%, to obtain the Centella asiatica extract.

[0088] Among them, the amount of bromelain added is 1200U / g product 1; the amount of glucose added is 3.02% of the mass of product 1; the amount of potassium hydrogen phosphate added is 0.065% of the mass of product 1;

[0089] The fermentation bacteria were Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322 in a ratio of 1:2; the fermentation bacteria dosage was 4.80×10 9 cfu / g product 1.

[0090] B. Preparation of Gentiana Extract

[0091] Gentiana Extract 1:

[0092] The preparation method comprises: (1) crushing dried gentian root, adding 45% ethanol aqueous solution with a mass concentration of 30 times the mass of gentian root, keeping warm at 60° C. and stirring at 70 rpm for 7.5 hours to obtain intermediate product 1;

[0093] (2) The intermediate product 1 is filtered using a filter membrane with a pore size of 1 micron, and the filtrate is ultrafiltered using an ultrafiltration membrane with a molecular weight cutoff of 500 Da. The ultrafiltrate is then concentrated under reduced pressure at 55° C. to 0.1 times the mass of the intermediate product 1, and freeze-dried to a water content of 2.91 wt %, to obtain the gentian extract.

[0094] Gentiana Extract 2:

[0095] The preparation method comprises: (1) crushing dried gentian root, adding 32 times the mass of gentian root and 52% ethanol aqueous solution, keeping warm at 60° C. and stirring at 70 rpm for 7.2 hours, to obtain intermediate product 1;

[0096] (2) The intermediate product 1 is filtered using a filter membrane with a pore size of 1 micron, and the filtrate is ultrafiltered using an ultrafiltration membrane with a molecular weight cutoff of 500 Da. The ultrafiltrate is then concentrated under reduced pressure at 55° C. to 0.105 times the mass of the intermediate product 1, and freeze-dried to a water content of 2.82 wt %, to obtain the gentian extract.

[0097] Gentiana Extract 3:

[0098] The preparation method comprises: (1) crushing dried gentian root, adding 30 times the mass of gentian root and 20% ethanol aqueous solution, keeping warm at 60° C. and stirring at 70 rpm for 7.5 hours, to obtain intermediate product 1;

[0099] (2) The intermediate product 1 is filtered using a filter membrane with a pore size of 1 micron, and the filtrate is ultrafiltered using an ultrafiltration membrane with a molecular weight cutoff of 500 Da. The ultrafiltrate is then concentrated under reduced pressure at 55° C. to 0.1 times the mass of the intermediate product 1, and freeze-dried to a water content of 2.91 wt %, to obtain the gentian extract.

[0100] Gentiana Extract 4:

[0101] The preparation method comprises: (1) crushing the dried gentian root, adding 30 times the mass of the gentian root and 70% ethanol aqueous solution, keeping warm at 60° C. and stirring at 70 rpm for 7.5 hours to obtain an intermediate product 1;

[0102] (2) The intermediate product 1 is filtered using a filter membrane with a pore size of 1 micron, and the filtrate is ultrafiltered using an ultrafiltration membrane with a molecular weight cutoff of 500 Da. The ultrafiltrate is then concentrated under reduced pressure at 55° C. to 0.1 times the mass of the intermediate product 1, and freeze-dried to a water content of 2.91 wt %, to obtain the gentian extract.

[0103] Gentiana Extract 5:

[0104] The preparation method comprises: (1) crushing dried gentian root, adding 45% ethanol aqueous solution with a mass concentration of 30 times the mass of gentian root, keeping warm at 60° C. and stirring at 70 rpm for 7.5 hours to obtain intermediate product 1;

[0105] (2) The intermediate product 1 is filtered using a filter membrane with a pore size of 1 micron, and the filtrate is ultrafiltered using an ultrafiltration membrane with a molecular weight cutoff of 1500 Da. The ultrafiltrate is then concentrated under reduced pressure at 55° C. to 0.1 times the mass of the intermediate product 1, and freeze-dried to a water content of 2.91 wt %, to obtain the gentian extract.

[0106] C. Extract inhibits inflammation and soothes the skin performance test

[0107] RAW264.7 cells were cultured at 1.2 × 10 5 The cells were inoculated into a 24-well cell culture plate at a density of 0.5 mL per well and cultured for 24 hours. Except for the blank control group, LPS was added to the cells of the other groups to a concentration of 1 μg / mL to establish a cell inflammation model. Among them, LPS was only added to the cells of the model group to a concentration of 1 μg / mL, while LPS was added to each experimental group to a concentration of 1 μg / mL and each extract diluent was added to the extract diluent to a concentration of 0.01% (wherein, the extract diluent was diluted to a mass concentration of 1% using deionized water); each group was incubated at 37°C for 24 hours. Then the upper culture fluid was collected, and the expression levels of IL-6 and TNF-α in each group were detected according to the instructions of the ELISA kit; each group was tested in parallel 5 times and the average value was taken. The results are shown in Table 1.

[0108] Table 1: Expression levels of IL-6 and TNF-α in each extract group

[0109]

[0110]

[0111] According to the test in Table 1, the Centella asiatica extract and the Gentiana dahurica extract prepared by the present invention have excellent effects of reducing the expression of IL-6 and TNF-α, can effectively inhibit inflammation, and have a good skin soothing effect.

[0112] According to the test of Centella asiatica extracts 1 and 3, adding bromelain during the fermentation process can effectively promote the extract to inhibit the increase of IL-6 and TNF-α expression. According to the test of extracts 1, 4-7, the combination of Lactobacillus reuteri CICC 6122 and Bacillus plantarum CICC 20322 can effectively improve the effect of inhibiting inflammation. The combination of the two can synergistically enhance the effect of inhibiting the expression of IL-6 and TNF-α. The co-fermentation of the two can promote the richness of soothing active ingredients and the subsequent concentration and other treatments can effectively enhance the soothing effect of the extract; and when the ratio of the two is 1:0.4-0.8, the effect is better.

[0113] According to the test of gentian extracts 1-5, the present invention uses 40-55% ethanol aqueous solution for extraction and uses an ultrafiltration membrane with a molecular weight cutoff of less than 800Da for interception treatment, which can effectively enhance the soothing effect of the extract and give the extract better performance.

[0114] D. Effect test of extract combination

[0115] The above-mentioned Centella asiatica extract 1 and gentian extract 1 were matched and tested. The test method was carried out according to C. The original test recorded that "LPS was added to each experimental group to a concentration of 1 μg / mL and each extract diluent was added to the extract diluent to a concentration of 0.01% (wherein, the extract diluent was diluted with deionized water to a mass concentration of 1%)" was replaced with "LPS was added to each experimental group to a concentration of 1 μg / mL and each extract matching diluent was added to the extract diluent to a concentration of 0.01% (wherein, the extract matching diluent was diluted with deionized water to a mass concentration of 1%)", and the rest remained unchanged. The specific matching ratios and test results are shown in Table 2. C represents a combination of Centella asiatica extract 1 and gentian extract 1 in different mass ratios.

[0116] Table 2: Expression levels of IL-6 and TNF-α in each group of extracts

[0117]

[0118]

[0119] According to the test in Table 2, when Centella asiatica extract 1 and Gentiana extract 1 are used together, they promote each other in a certain ratio range, but produce antagonistic effects in other ratio ranges. Therefore, when choosing a combination with excellent soothing properties, the best effect is achieved when the mass ratio of Centella asiatica extract to Gentiana extract is 0.8-1:1.

[0120] 2. Preparation and testing of cosmetic spray

[0121] 1. Preparation of spray

[0122] Prepare the raw materials for each spray according to the ratio in Table 3 and measure them by mass percentage. The ratio K value is the mass ratio of 1,3-butylene glycol and PEG-10 tris(trimethylsiloxy)silylethyl polydimethylsiloxane.

[0123] Table 3: Spray composition

[0124]

[0125]

[0126] The preparation method of the above spray is as follows:

[0127] Cyclopentasiloxane, caprylyl polymethicone, polydimethicone, butylene glycol, polymethylsilsesquioxane, ethylhexyl salicylate, diethylaminohydroxybenzoyl hexyl benzoate, octocrylene, lauryl PEG-10 tri(trimethylsiloxy)silylethyl polydimethicone and ethylhexyl triazone are added into a container, heated to 82°C, and stirred to mix; then water is added, and stirring is continued for 30 minutes while maintaining the speed unchanged; the container is cooled to 28°C, and Centella asiatica extract and gentiana extract are added (this step is not used in spray 5), and stirring is continued for 15 minutes while maintaining the speed unchanged to mix, and sterilize to obtain a spray.

[0128] 2. Spray test

[0129] The above spray was tested for its sun protection effect, usage experience and stability.

[0130] Sunscreen effect test: Use UV-2000S cosmetic sun protection index analyzer for testing. The specific steps are: weigh the test sample, apply the test sample evenly on the sample plate, place it in a cool place to dry naturally for 30 minutes, and then test it to get the SPF value. The larger the SPF value, the better the sunscreen effect.

[0131] Usage experience test: 10 volunteers were selected to conduct sensory tests on different sprays in turn for a refreshing and non-greasy feeling. The highest score was 10 points and the lowest score was 1 point. The average score of each spray was then calculated.

[0132] Stability test: Place 50 mL of the spray in a transparent glass bottle and place it at 55°C for 1 week to observe the appearance of the spray.

[0133] The specific test results are shown in Table 4.

[0134] Table 4: Spray sunscreen, usage experience and stability test

[0135] test Sun protection SPF value User experience rating stability Spray 1 47.6 9.6 No stratification, no precipitation Spray 2 47.4 9.5 No stratification, no precipitation Spray 3 46.2 8.9 No stratification, slight floccules Spray 4 45.1 8.5 Turbid appearance Spray 5 46.8 9.4 No stratification, no precipitation

[0136] According to the test in Table 4, the spray sunscreen SPF value prepared by the present invention reaches 47.6, the refreshing and non-greasy score reaches 9.6, and there is no stratification and no precipitation after being placed at 55°C for one week; the spray of the present invention has an excellent sunscreen effect, is refreshing and non-greasy after application, and has good temperature resistance and stability.

[0137] According to the spray 1, 3-4 tests, even when the amount of emulsifier PEG-10 tris(trimethylsiloxy)silylethyl polydimethicone is increased or the amount of butylene glycol is increased, when the ratio of butylene glycol to PEG-10 tris(trimethylsiloxy)silylethyl polydimethicone is not within the range of 1.5-2.2:1, the product uniformity is poor, resulting in poor distribution of active ingredients and poor use effect, which leads to a decrease in the SPF test value, and also reduces the product stability and use effect.

[0138] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A soothing and sunscreen spray, characterized in that: The ingredients include cyclopentasiloxane, caprylyl methicone, dimethicone, butylene glycol, polymethylsilsesquioxane, ethylhexyl salicylate, diethylamino hydroxybenzoyl hexyl benzoate, octocrylene, lauryl PEG-10 tris(trimethylsiloxy) silylethyl polydimethylsiloxane, ethylhexyl triazone and water.

2. The soothing and sunscreen spray according to claim 1, characterized in that: According to the mass percentage, the raw materials include: 1-5% cyclopentasiloxane, 0.5-3% octyl polymethylsiloxane, 0.5-2% polydimethylsiloxane, 8-12% butylene glycol, 0.5-2% polymethylsilsesquioxane, 0.4-1.5% ethylhexyl salicylate, 0.3-1.5% diethylaminohydroxybenzoyl hexyl benzoate, 0.3-1.5% octocrylene, 4-8% lauryl PEG-10 tri(trimethylsiloxy) silylethyl polydimethylsiloxane, 0.4-1.5% ethylhexyl triazone and the balance water.

3. The soothing and sunscreen spray according to claim 1, characterized in that: Also included is 0.5-2% Centella asiatica extract and 0.5-1.8% Gentiana dasyphylla extract.

4. The soothing and sunscreen spray according to claim 4, characterized in that: The mass ratio of the Centella asiatica extract to the Gentiana dahurica extract is 0.8-1:

1.

5. The soothing and sunscreen spray according to claim 1, characterized in that: The mass ratio of butylene glycol to lauryl PEG-10 tris(trimethylsiloxy)silylethyl polydimethicone is 1.5-2.2:1; And / or, the Centella asiatica extract is an extract prepared by a process comprising enzymatic hydrolysis of Centella asiatica with bromelain and fermentation with fermentation bacteria, and the gentiana extract is an extract prepared by a process comprising extraction with a 40-55% ethanol solution and ultrafiltration of gentiana root.

6. The soothing and sunscreen spray according to claim 3, characterized in that: The preparation method of the Centella asiatica extract comprises: Step 1: Crush the dried whole herb of Centella asiatica, add water, heat and keep warm to obtain product 1; Step 2: sterilize product 1, add bromelain, glucose, dipotassium hydrogen phosphate and fermentation bacteria, ferment and sterilize to obtain product 2; Step 3: Filter the product 2 to obtain a filtrate, and dry it to obtain the Centella asiatica extract.

7. The soothing and sunscreen spray according to claim 6, characterized in that: The amount of bromelain added is 600-1500U / g product 1; and / or, the amount of glucose added is 1-4% of the mass of product 1; and / or, the amount of potassium hydrogen phosphate added is 0.02-0.1% by mass of the product 1; and / or, the fermentation bacteria are Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322 in a quantitative ratio of 1:0.4-0.8; And / or, the fermentation bacteria dosage is 1×10 9 -9×10 9 cfu / g product 1.

8. The soothing and sunscreen spray according to claim 3, characterized in that: The preparation method of gentian extract comprises: (1) Crush the dried gentian root, add 8-40 times the mass of ethanol aqueous solution of the gentian root, heat at 50-65° C. and stir for 3-10 hours to obtain an intermediate product 1; (2) filtering the intermediate product 1, ultrafiltration, and then concentrating under reduced pressure to 0.05-0.12 times the mass of the intermediate product, and drying to obtain the gentian extract; The mass concentration of the ethanol aqueous solution is 40-55%; the ultrafiltration is performed by using an ultrafiltration membrane with a molecular weight cutoff lower than 800Da.

9. A method for preparing the soothing and sunscreen spray according to any one of claims 1 to 8, characterized in that: The method comprises the following steps: adding cyclopentasiloxane, octyl polymethicone, polydimethicone, butylene glycol, polymethylsilsesquioxane, ethylhexyl salicylate, diethylaminohydroxybenzoyl hexyl benzoate, octocrylene, lauryl PEG-10 tris(trimethylsiloxy)silylethyl polydimethicone and ethylhexyl triazone into a container, heating to 60-85°C, stirring and mixing; then adding water and stirring; cooling, then adding Centella asiatica extract and gentiana extract and stirring and mixing, and sterilizing to obtain the soothing and sunscreen spray.

10. Use of the soothing and sunscreen spray according to any one of claims 1 to 8 in non-therapeutic cosmetics.

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