Method for analyzing content of adenosine and cordycepin in cordyceps militaris by virtue of high performance liquid chromatography (HPLC)

Abstract

The invention discloses a method for analyzing the content of adenosine and cordycepin in the sporocarp of cordyceps militaris through artificial culture by virtue of high performance liquid chromatography (HPLC). The method comprises the following specific steps: 1, weighing the dried sporocarp powder of cordyceps militaris in a 50ml centrifuge tube with a plug, adding water, ultrasonically extracting the centrifuge tube in water bath, then centrifuging and taking a supernate as a test solution; 2, weighing a certain amount of standard substances of adenosine and cordycepin, adding water and dissolving to prepare a mixed solution as a mixed standard solution; 3, performing content measuring, namely taking the mixed standard solution and test solution respectively, filtering the solutions through a 0.45um aqueous phase millipore filter and injecting the filtered solutions into a small bottle, and measuring the chromatographic peak of the cordyceps militaris sporocarp test solution and the adenosine cordycepin standard solution with the same chromatographic retention time. The invention provides a new, simple and convenient method for evaluating the quality of the cordyceps militaris sporocarp.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for analyzing the contents of adenosine and cordycepin in fruit bodies of Cordyceps militaris by high performance liquid chromatography (HPLC). Background technique [0002] Cordyceps militaris (also known as Cordyceps militaris, Cordyceps militaris) is the dry sub-seat of Cordyceps militaris (L.) Link, a fungus of the genus Cordyceps of the family Ergotaceae. Anti-arrhythmia, anti-hypertensive, anti-pathogenic microorganisms, anti-malignant tumors and other pharmacological activities. Nucleoside components such as adenosine and cordycepin are used as the main reference indicators for the quality of Cordyceps. Adenosine (C 10 h 13 N 5 o 4 , the structural formula shown below (a)) is the full name of adenosine, which is the basic structural unit of nucleic acid, and it has the function of participating in human metabolism and physiological regulation. Cordycepin ...

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Problems solved by technology

Liu Xiaofang et al. (Liu Xiaofang, Xue Changhu, Chang Yaoguang. Determination of adenosine and cordycepin in Cordyceps militaris by high performance liquid chromatography[J]. Food Science, 2010,31(16):172-175) proposed a A new method for determining the content of adenosine and cordycepin in Cordyceps militaris. The mobile phase is composed of citric acid, acetic acid, triethylamine mixed solution and acetonitrile. The composition and preparation process of the mobile phase are relatively complicated

Li Xiangling et al. (Li Xiangling, Hu Jinsong, Chen Zuohong. HPLC determination of cordycepin and adenosine content in artificial Cordyceps militaris and its medium [J]. Journal of Natural Science of Hunan Normal University, 2010,33(2):107-110), Mao Xinliang et al. (Mao Xinliang, Zheng Guodong, Zhang Chen, et al. Research on the simultaneous determination of adenosine, cordycepin, and muscle liver in Cordyceps sinensis by reversed-phase high-performance liquid chromatography[J]. Zhongnan Pharmacy, 2009, 7(12): 895-897), although methanol and water are used as the mobile phase, the gradient elution method is used, and the process is cumbersome

In addition, if the fruiting body of Cordyceps militaris is extracted using the extraction conditions of adenosine in the 2003 edition of "Technical Standards for Inspection and Evaluation of Health Food", the extraction rate is low

[0004] At present, there is no unified national standard method for the determination of adenosine and cordycepin in Cordyceps militaris, so a simple and reliable method for simultaneous analysis of adenosine by one-time sample injection is established and the content of Cordyceps militaris have practical significance

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