Method for analyzing content of adenosine and cordycepin in cordyceps militaris by virtue of high performance liquid chromatography (HPLC)

A technology of high performance liquid chromatography and cordycepin, which is applied in the direction of analyzing materials, measuring devices, and separating materials
CN102928526AInactive Publication Date: 2013-02-13SHANGHAI JIAOTONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI JIAOTONG UNIV
Publication Date
2013-02-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for analyzing the content of adenosine and cordycepin in the sporocarp of cordyceps militaris through artificial culture by virtue of high performance liquid chromatography (HPLC). The method comprises the following specific steps: 1, weighing the dried sporocarp powder of cordyceps militaris in a 50ml centrifuge tube with a plug, adding water, ultrasonically extracting the centrifuge tube in water bath, then centrifuging and taking a supernate as a test solution; 2, weighing a certain amount of standard substances of adenosine and cordycepin, adding water and dissolving to prepare a mixed solution as a mixed standard solution; 3, performing content measuring, namely taking the mixed standard solution and test solution respectively, filtering the solutions through a 0.45um aqueous phase millipore filter and injecting the filtered solutions into a small bottle, and measuring the chromatographic peak of the cordyceps militaris sporocarp test solution and the adenosine cordycepin standard solution with the same chromatographic retention time. The invention provides a new, simple and convenient method for evaluating the quality of the cordyceps militaris sporocarp.
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Description

technical field

[0001] The invention relates to the field of analytical chemistry, in particular to a method for analyzing the contents of adenosine and cordycepin in fruit bodies of Cordyceps militaris by high performance liquid chromatography (HPLC). Background technique

[0002] Cordyceps militaris (also known as Cordyceps militaris, Cordyceps militaris) is the dry sub-seat of Cordyceps militaris (L.) Link, a fungus of the genus Cordyceps of the family Ergotaceae. Anti-arrhythmia, anti-hypertensive, anti-pathogenic microorganisms, anti-malignant tumors and other pharmacological activities. Nucleoside components such as adenosine and cordycepin are used as the main reference indicators for the quality of Cordyceps. Adenosine (C 10 h 13 N 5 o 4 , the structural formula shown below (a)) is the full name of adenosine, which is the basic structural unit of nucleic acid, and it has the function of participating in human metabolism and physiological regulation. Cordycepin ...

Claims

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