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Squash polysaccharide and preparation method thereof

A technology for pumpkin polysaccharide and pumpkin, which is applied to the field of pumpkin polysaccharide and its preparation, can solve the problems of low polysaccharide extraction rate, long production cycle, long extraction time and the like, and achieves the effects of simple technological process, low production cost and optimized extraction conditions.

Inactive Publication Date: 2008-12-03
卢照凯 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the disadvantages of long extraction time, complex process and cumbersome process, the precipitate needs to be washed many times, and after adding trichloroacetic acid to deproteinize, it needs to undergo reverse water dialysis and double distilled water dialysis, the production cycle is long, and the extraction rate of polysaccharides is low. The problem of low purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 of the present invention: the preparation method of pumpkin polysaccharide, its steps are as follows:

[0018] 1) Select 100kg of pumpkins with sweet taste, rich starch, smooth appearance, hard and thick flesh, and no mold and mildew. Rinse well, cut to remove seeds, cut into pieces with a length of 5 cm, a width of 2 cm, and a thickness of 2.5 cm, and place them in a pulverizer to pulverize into 80-mesh granules to obtain crushed pumpkin.

[0019] 2) Put the crushed pumpkin in the extraction tank, add 600kg of purified water, stir and mix evenly, then input steam, raise the temperature of the water in the tank to 95°C, and keep warm for 1.5h.

[0020] 3) Filter with a 180-mesh filter, collect the filtrate in a liquid storage tank, and obtain filtrate A. Add 550kg of purified water to the filtered filter residue, stir and mix, then input steam, raise the water temperature to 95°C, and keep warm for 1.5h. Filter with a 180-mesh filter, collect the filtrate...

Embodiment 2

[0027] Embodiment 2 of the present invention: prepare pumpkin polysaccharide according to the following steps:

[0028] 1) Select 150kg of pumpkins with smooth appearance, hard and thick flesh, mature and no mold, remove the stalks, pedicles and parts bitten by insects, scrub with a brush first, then rinse with clean drinking water, cut and remove The seeds are cut into 6cm long, 3cm wide, and 2.2cm thick slices, and are pulverized into 150 mesh particles with a pulverizer to obtain pumpkin pulverization.

[0029] 2) Put the crushed pumpkin in an extraction tank, add 750kg of deionized water, stir and mix evenly, then input steam, raise the temperature of the water in the tank to 92°C, and keep warm for 1.8h.

[0030] 3) Filter with a 120-mesh filter, collect the filtrate in a liquid storage tank to obtain filtrate A. Add 650kg of deionized water to the filtered filter residue, stir and mix, then input steam, raise the water temperature to 92°C, and keep warm for 1.8h. Filte...

Embodiment 3

[0037] Embodiment 3 of the present invention: prepare pumpkin polysaccharide according to the following steps:

[0038] 1) Select 220kg of mature, mildew-free pumpkins, remove the stalks and pedicles and remove insect bites, scrub with a rag, rinse with clean drinking water, cut and remove seeds, cut into 4.5cm in length and 3.6cm in width 1. The sheet with a thickness of 3 cm is placed in a pulverizer and pulverized into 160 mesh particles to obtain pumpkin pulverization.

[0039] 2) Put the crushed pumpkin in an extraction tank, add 1000kg of drinking water, stir and mix evenly, then input steam, raise the temperature of the water in the tank to 85°C, and keep warm for 2 hours.

[0040] 3) Filter with a 140-mesh filter, collect the filtrate in a liquid storage tank, and obtain filtrate A. Add 900kg of drinking water to the filtered filter residue, stir and mix, then input steam, raise the water temperature to 85°C, and keep warm for 2 hours. Filter with a 140-mesh filter, co...

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PUM

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Abstract

The invention discloses pumpkin polysaccharide and the preparation method thereof. The preparation method comprises the steps as follows: pre-treating pumpkin, extracting in water for 0.5-3h, filtering to obtain filtrate, centrifuging the filtrated supernatant, purifying the supernatant with a resin column chromatography, and colleting eluent; concentrating the eluent, and precipitating with ethanol; and dissolving the precipitate into water, adding trichloroacetic acid, centrifuging for deproteinization to obtain supernatant, concentrating the supernatant, drying and pulverizing. The washing number of times is reduced, the technological process is simplified, and the extraction condition and technological parameter are optimized to shorten the time of the extraction and purification processes by purifying the pumpkin polysaccharide with advanced resin chromatography technology. Therefore, the preparation method has the advantages of simple technological process, short production period, easily-controlled production, and low production cost. The purity of the obtained pumpkin polysaccharide is as high as more than 80%, and the extraction rate is as high as more than 25%.

Description

(1) Technical field: [0001] The invention relates to a pumpkin polysaccharide and a preparation method thereof, belonging to the technical field of extraction of medicinal active ingredients. (two) background technology: [0002] Pumpkin not only has very high nutritional value, but also has extremely high medicinal value, among which the most well-known medicinal and health functions are anti-diabetic. The polysaccharides and other ingredients contained in pumpkin are the main effective substances for anti-diabetes. At present, the anti-diabetic effect of pumpkin is used, and the pumpkin is usually dehydrated, dried and ground to make pumpkin powder and then taken after mixing. The contained medicinal ingredient of the product that this method makes is low, and dosage is large, and curative effect is not remarkable, and taking is convenient enough. In recent years, there have been public reports of extracting and isolating pumpkin polysaccharides with obvious hypoglycemic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P3/10
Inventor 卢照凯卢昶年
Owner 卢照凯
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