Kit for quantitatively detecting GFAP concentration in human serum by polystyrene microsphere

A polystyrene microsphere, quantitative detection technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of short time consumption, shortened reaction time, and reduced washing times
CN102411056AActive Publication Date: 2012-04-11山东莱博生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
山东莱博生物科技有限公司
Publication Date
2012-04-11

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Abstract

The invention discloses a kit for quantitatively detecting GFAP concentration in human serum by polystyrene microsphere, comprising a 96-hole filter plate, a standard product, a sample diluent, a polystyrene microsphere coupled with an anti-GFAP antibody on the surface, a microsphere storage solution, an enzyme compound, a substrate A, a substrate B, a 20*cleaning solution, an adhesive sticker strip and an operating manual. The kit provided by the invention changes the solid phase carrier made of traditional microporous plate into the polystyrene microsphere, generates the antigen antibody reaction in the liquid phase to keep a good bioactivity and a correct spatial configuration, and eliminates the effect on the antigen antibody reaction by the surface tension and the steric hindrance ofthe traditional reaction mode, and effectively shortens the reaction time and the rinsing times, besides, the invention has the characteristics of simple operation, high sensitivity, good specificityand low cost and has important clinical significance for the diagnosis of the acute cerebral hemorrhage early disease degree.
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Description

technical field

[0001] The invention relates to a kit for quantitatively detecting the concentration of glial fibrillary acidic protein (GFAP) in serum, in particular to a kit for quantitatively detecting the concentration of glial fibrillary acidic protein (GFAP) in human serum by using anti-GFAP antibody-specifically coated polystyrene microspheres. GFAP concentration kit. Background technique

[0002] Epidemiological surveys show that cerebrovascular disease, heart disease, and malignant tumors constitute the three major causes of human death. Compared with developed countries in the West, the morbidity and mortality of cerebrovascular diseases in my country are significantly higher than those of cardiovascular diseases. It is estimated that there are about 2 million new stroke patients in the country every year; about 1.5 million patients die of stroke every year; the number of surviving patients is 6 million to 7 million, and 70% to 80% of the survivors are left with p...

Claims

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