Connection type microbial cultivation device with embedded millipore filter and culturing method thereof

A microbial culture and microbial culture medium technology, applied in the field of connected microbial culture devices, can solve the problems of inability to simulate the environmental conditions of microorganisms well, the limited connection between the microorganisms to be cultured and the external environmental medium, and the inability to produce large-scale industrialization. Inexpensive and the effect of improving cultivability

Inactive Publication Date: 2008-01-23
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] The present invention aims to overcome the shortcomings of the culture devices used in the current microbial culture process: 1) the microorganisms to be cultured have limited contact with the external environment medium, and cannot simulate t...

Method used

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  • Connection type microbial cultivation device with embedded millipore filter and culturing method thereof
  • Connection type microbial cultivation device with embedded millipore filter and culturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] For the microbial samples from the water environment, the connected microbial culture device with built-in microporous membrane is used for culture.

[0038] Figure 1 is a schematic diagram of the structure of a cell-based microbial culture device. Among them, the dish cover 1 is the same as the traditional petri dish, and is buckled upside down on the dish body 2 to isolate the inside of the cultivating device from the outside world and keep the interior free from bacterial contamination; the partition 3 is placed in the inner space of the dish body 2, and the microbe The porous filter membrane 4 divides the inner space of the petri dish body 2 into two parts, one part carries the culture medium of microorganisms to be separated. The other part bears the medium of simulating the external environment, and plays a supporting role to the agar medium. The material of the partition 3 is the same as the dish body 2 and the dish cover 1, and is connected with the dish body 2 ...

Embodiment 2

[0045] For the microbial samples from the sediment environment, a connected microbial culture device with a built-in microporous membrane is used for culture.

[0046] When using a cell-type microbial culture device for culturing, use the balanced water in the upper layer of the sediment from the sample source as the main component of the microbial culture medium, and also contain 1.5% (w / v) agar, 1g / L peptone and 0.2g / L yeast extract. Pour the medium into the space of the medium in the petri dish, and wait for the medium to cool and condense. Add the microorganisms to be cultured on the culture medium for coating, or streak culture. An appropriate amount of the sediment is added to the balanced water body on the upper layer of the sediment from which the sample originates as a medium for simulating the external environment, and placed in a petri dish to carry the medium for simulating the external environment. After the sediment is naturally deposited on the bottom and stabi...

Embodiment 3

[0050] For microbial samples derived from the soil environment, a connected microbial culture device with a built-in microporous membrane is used for cultivation.

[0051] When using a cell-type microbial culture device for culturing, the source of the sample is fully balanced sterile saline (0.9% NaCl) as the main component of the microbial culture medium, and additionally contains 1.5% (w / v) agar, 1g / L peptone and 0.2 g / L yeast paste. Pour the medium into the space of the medium in the petri dish, and wait for the medium to cool and condense. Add the microorganisms to be cultured on the culture medium for coating, or streak culture. Add an appropriate amount of the soil to the sterile saline that has been fully balanced from the source of the sample, as a medium for simulating the external environment, and place it in a petri dish to carry the medium for simulating the external environment. After the soil is naturally deposited on the bottom and stabilized, cover the dish ...

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PUM

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Abstract

The invention discloses a built-in microporous filter membrane connection microbe culture device belonging to the microbe culture technology, a culture method thereof, and a microbe culture, isolation and purification and observation technology in various environments by utilizing a culture medium. The culture device is provided with clapboard in an internal space composed of a dish body and a dish body lid, and the clapboard is fixed with a microporous filter membrane which divides the internal space of the culture device into two spaces. The two spaces are respectively used to bear the microbial culture group and provide a simulation external environment medium of a microchemistry matter with a microbial growth necessary factor. The dissolved matters at the two sides of the filter membrane are capable of freely exchange and the cell cannot permeate. Therefore, the culture device is capable of culturing more miceobes that the regular method can not culture. The invention applies the culture device to culture the microbes, which can effectively improve the culturablity of the environmental microbes, facilitate experiment system construction, make the operation easy and time-saving and has stronger industrialized application potention.

Description

technical field [0001] The invention belongs to the laboratory cultivation technology of microorganisms, and relates to a connected microorganism cultivation device with a built-in microporous filter membrane and a cultivation method thereof. Specifically, it involves the design of a new type of microbial culture device that improves the cultivability of microorganisms and the technology of cultivating, separating, purifying, and observing environmental microorganisms using solid or semi-solid medium. Background technique [0002] For a long time, people have used pure culture technology to investigate, research, develop and utilize microorganisms in the environment. However, studies have shown that the vast majority of microorganisms in nature cannot be cultured by current pure culture techniques. The main reason is that the culture medium for cultivating microorganisms is very different from the medium of the actual environment where the microorganisms are located, result...

Claims

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Application Information

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IPC IPC(8): C12M3/06
Inventor 吴晓磊郭斌余素林
Owner TSINGHUA UNIV
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