The invention relates to the technical field of
tissue engineering and medical wound repairing, in particular to an
amnion innate
stem cell carried frozen active
amnion particle and a
conditioned medium and application thereof. Discarded fresh amnions are prepared into particles and then frozen into
liquid nitrogen by means of serum-free
stem cell freezing liquid; thus, complete matrix components of
amnion are maintained while amnion epithelial
cell activity is maintained for a long time, and further,
disease transmission is avoided effectively. The
conditioned medium which is prepared and collected by means of the frozen active amnion particles can promote
chemotaxis and migration of human epidermal cells,
fibroblast and endothelial cells effectively. In terms of zoografting,
wound healing can be improved by multiple ways of regulating inflammatory reaction, promoting vascularization, quickening epithelization and the like through the active amnion particles; further, the amnion matrix can be directly used as a
dermis equivalent to induce dermal regeneration so as to improve
wound healing quality remarkably. The amnion innate
stem cell carried frozen active amnion particle and the
conditioned medium thereof can provide simple but effective ways for wound repairing.