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Method for detecting biochemical oxygen demand

A technology of biochemical oxygen demand and detection method, applied in the field of biochemical oxygen demand detection, can solve the problems of high price, secondary pollution of the environment, unsuitable for online monitoring, etc., to achieve easier removal, high biodegradation rate, and self-realization repaired effect

Active Publication Date: 2012-07-25
吉林市光大分析技术有限责任公司
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above methods for detecting BOD in water bodies are all based on the action of microorganisms on organic matter, including the respiration of microorganisms and the degradation of organic matter by microorganisms. In order to maintain the physiological activity of microorganisms and provide them with a certain pH value environment and osmotic pressure, the existing Technology provides a buffer solution system for the survival of microorganisms. At present, the most commonly used buffer solutions are phosphate buffer solution and Tris-HCl buffer system. Among them, phosphate buffer solution will cause secondary pollution to the environment, and Tris-HCl buffer system is expensive. Suitable for online monitoring
In the prior art, there is no method for detecting BOD in water without using a buffer system

Method used

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  • Method for detecting biochemical oxygen demand
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  • Method for detecting biochemical oxygen demand

Examples

Experimental program
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Effect test

Embodiment 1

[0115] according to figure 1 Schematic diagram of the preparation of the microbial membrane reactor is shown. One end of the microbial membrane reactor 8 is connected to the oxygen electrode 5, and the oxygen electrode 5 is connected to the electrochemical workstation 3 whose model is CHI832b. The electrochemical workstation 3 monitors the current change of the oxygen electrode 5 and displays it by the computer 1.

[0116] Turn on the constant temperature water bath 20 and adjust the temperature to 30°C. Inject 300 mL of activated sludge water sample from water source 18 into actual water sample container 14 through sample pump 17, and carry out air saturation to the activated sludge water sample at a rate of 3.0 L / min. A glass reactor 8 with a length of 105.0 cm and an inner diameter of 2.0 mm is placed in a constant temperature water bath, and the water sample of the activated sludge passes through the first sampling pipe 11, the solenoid valve 10 and the sampling pipe at a...

Embodiment 2

[0118] Turn on the constant temperature water bath 20 and adjust the temperature to 30°C. 300 mL of tap water was poured into the tap water container 15, and it was air-saturated at a rate of 3.0 L / min. Under the control of the peristaltic pump 9, the air-saturated tap water passes through the second sampling pipe 12, the solenoid valve 10, and the sampling pipe 19 at a rate of 1.0 mL / min, reaches the microbial film reactor, and flows through the microbial film After the reactor reaches the surface of the oxygen electrode 5 . The electrochemical workstation 3 monitors the change of the oxygen electrode current, and when the oxygen electrode current is stable, the recorded dissolved oxygen reduction current is 539nA. .

[0119] Dissolve 150mg of glucose and 150mg of glutamic acid in tap water and set the volume to 100mL to obtain a BOD concentration of 1980.0mg O 2 / L of the mother liquor of the glucose glutamate solution. The mother liquor is diluted with tap water to obta...

Embodiment 3

[0122] Turn on the constant temperature water bath 20 and adjust the temperature to 30°C. 300 mL of tap water was poured into the tap water container 15, and it was air-saturated at a rate of 3.0 L / min. Under the control of the peristaltic pump 9, the obtained air-saturated tap water flows through the second sampling tube 12, the solenoid valve 10 and the sampling tube 19 at a rate of 1.0 mL / min, reaches the reactor 8, and flows through the reactor 8. Finally, it reaches the surface of the oxygen electrode 5 . The electrochemical workstation 3 monitors the change of the oxygen electrode current, and when the oxygen electrode current is stable, the recorded dissolved oxygen reduction current is 538nA.

[0123] The water samples in the secondary sedimentation tank of Changchun No. 2 Sewage Treatment Plant were air-saturated at a rate of 3.0 L / min. Under the control of the peristaltic pump 9, the water sample in the actual water sample container 14 flows through the first sampl...

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Abstract

The invention provides a method for detecting a biochemical oxygen demand. The method comprises the following steps of performing microculture on a microbe containing water sample from active sludge, surface water, domestic sewage or microbe containing industrial waste water, thus obtaining a microbial film; enabling a blank water sample and a target water sample to pass through the microbial film respectively, detecting and obtaining dissolved oxygen reduction current of the blank water sample and the target water sample, thus obtaining a dissolved oxygen reduction current difference value between the target water sample and the blank water sample; and obtaining the biochemical oxygen demand of the target water sample according to the dissolved oxygen reduction current difference value and a preset standard curve. According to the method, since the microbial film obtained by culturing the microbe containing water sample from active sludge, surface water, domestic sewage or microbe containing industrial waste water has high environment adaptation capacity, a buffer solution system is not required and the physiological activity can be maintained by taking one or more then one of running water, well water, dropping water and underground water as a medium.

Description

technical field [0001] The invention relates to the technical field of environmental protection, in particular to a method for detecting biochemical oxygen demand. Background technique [0002] Biochemical oxygen demand (BOD) refers to the amount of dissolved oxygen consumed by microorganisms to decompose some oxidizable substances in water, especially organic substances. If the time for biological oxidation is five days, it is called five-day biochemical oxygen demand. (BOD 5 ). Biochemical oxygen demand is an important indicator for analyzing the content of organic pollutants in water bodies and one of the most important parameters in routine monitoring of water quality. The higher the value, the more organic pollutants in the water and the more serious the pollution. [0003] At present, the dilution and inoculation method is generally used in the determination of biochemical oxygen demand in the world, also known as BOD. 5 However, this method is time-consuming, heavy...

Claims

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Application Information

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IPC IPC(8): G01N27/26
Inventor 刘长宇董绍俊
Owner 吉林市光大分析技术有限责任公司
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