Method for detecting mixing uniformity of soil heavy metal pollution samples
A technology for mixing uniformity and detection method, applied in the measurement of color/spectral characteristics, etc., can solve the problems of uniformity not meeting experimental requirements, low precision, errors, etc., to achieve rich raw materials, improve accuracy, and achieve accurate detection Effect
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[0029] 1. Using methyl violet as a tracer to test its own stability and interference with sample components
[0030] (1) Full-wavelength scanning of methyl violet
[0031] Dissolve 0.1 g (accurate to 0.001 g) of methyl violet solid in 1 L of absolute ethanol to prepare a 100 mg / L solution; absorb 200 μL of the solution, and use a microplate reader to perform full-wavelength scanning at 400-700 nm. Determine the best absorption wavelength of methyl violet is 579nm.
[0032] (2) Full-wavelength scanning of sample components
[0033] Take 10g of the soil and the heavy metal ion raw material as the total amount, and weigh the heavy metal ion according to the formula weight ratio: soil=1:1000 (respectively accurate to 0.001g), dissolve with absolute ethanol and set the volume to 100mL, 9000r / min Centrifuge for 5 minutes, filter the supernatant, draw 200 μL of the sample solution, and use a microplate reader to scan at a full wavelength of 400-700 nm.
[0034] (3) Detection of th...
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