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Non-genetically modified light control bidirectional regulation method for human umbilical cord mesenchymal stem cell proliferation

A technology of mesenchymal stem cells and light manipulation, applied in the field of mesenchymal stem cell culture application, can solve the problems of non-involvement, and achieve the effect of obvious effect and broad clinical application prospect.

Active Publication Date: 2018-10-09
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing factors that affect the proliferation of umbilical cord mesenchymal stem cells do not involve non-genetic modification light manipulation technology, and do not involve the bidirectional (negative inhibition, positive To promote) the technical method of regulating cell proliferation

Method used

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  • Non-genetically modified light control bidirectional regulation method for human umbilical cord mesenchymal stem cell proliferation
  • Non-genetically modified light control bidirectional regulation method for human umbilical cord mesenchymal stem cell proliferation
  • Non-genetically modified light control bidirectional regulation method for human umbilical cord mesenchymal stem cell proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Primary culture of human umbilical cord mesenchymal stem cells

[0048] Table 1: Composition of Proliferation Medium

[0049]

[0050] 2. Subculture of human umbilical cord mesenchymal stem cells

[0051] 1. Observe the cell growth status and cell density under a microscope. When the cell density reaches 80% to 90%, perform cell passaging operations on the ultra-clean workbench.

[0052] 2. Put the cell culture medium, PBS solution, and trypsin solution into a water bath for half an hour, then remove them from the water bath, wipe the surface with 75% alcohol, and transfer to the ultra-clean workbench.

[0053] 3. Use a vacuum pump to suck off the original culture medium in the petri dish, add an appropriate amount of PBS solution to wash the residual culture medium, and vacuum pump to remove it. Repeat the operation three times. After discarding the PBS solution for the last time, add 1 mL of trypsin solution to digest the cells.

[0054] 4. About 2-3min, the digestion is com...

Embodiment 2

[0085] 1. Primary culture of human umbilical cord mesenchymal stem cells

[0086] Table 1: Composition of Proliferation Medium

[0087]

[0088] 2. Subculture of human umbilical cord mesenchymal stem cells

[0089] 1. Observe the cell growth status and cell density under a microscope. When the cell density reaches 80% to 90%, perform cell passaging operations on the ultra-clean workbench.

[0090] 2. Put the cell culture medium, PBS solution, and pancreatin solution in a water bath for half an hour, then remove them from the water bath, wipe the surface with 75% alcohol, and transfer to the ultra-clean workbench.

[0091] 3. Use a vacuum pump to suck off the original culture medium in the petri dish, add an appropriate amount of PBS solution to wash the residual culture medium, and vacuum pump to remove it. Repeat the operation three times. After discarding the PBS solution for the last time, add 1 mL of trypsin solution to digest the cells.

[0092] 4. About 2-3 minutes, the digestion ...

Embodiment 3

[0123] 1. Primary culture of human umbilical cord mesenchymal stem cells

[0124] Table 1: Composition of Proliferation Medium

[0125]

[0126] 2. Subculture of human umbilical cord mesenchymal stem cells

[0127] 1. Observe the cell growth status and cell density under a microscope. When the cell density reaches 80% to 90%, perform cell passaging operations on the ultra-clean workbench.

[0128] 2. Put the cell culture medium, PBS solution, and trypsin solution into a water bath for half an hour, then remove them from the water bath, wipe the surface with 75% alcohol, and transfer to the ultra-clean workbench.

[0129] 3. Use a vacuum pump to suck off the original culture medium in the petri dish, add an appropriate amount of PBS solution to wash the residual culture medium, and vacuum pump to remove it. Repeat the operation three times. After discarding the PBS solution for the last time, add 1 mL of trypsin solution to digest the cells.

[0130] 4. About 2-3 minutes, the digestion i...

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Abstract

The invention relates to a non-genetically modified light control bidirectional regulation method for human umbilical cord mesenchymal stem cell proliferation. The method includes the steps: irradiating human umbilical cord mesenchymal stem cells at 95-105mm positions under a collimating mirror for 85-95min through the collimating mirror by the aid of blue light under the conditions of the light wavelength of 400-480nm and the light intensity of 95-105 micro-w / cm<2>, and performing negative regulation; or irradiating the human umbilical cord mesenchymal stem cells at the 95-105mm positions under the collimating mirror for 115-125min through the collimating mirror by the aid of blue light, and performing positive regulation. According to the method, the multiplication speed of the human umbilical cord mesenchymal stem cells is firstly and bi-directionally regulated only through blue light irradiation under setting of special parameters, foreign genes transferred into the stem cells areomitted, virus transfection vectors are omitted, any other substances are omitted, and the method more meets clinical application requirements of 'noninvasive light therapy' and has a wide clinical application prospect.

Description

Technical field [0001] The invention relates to a method for bidirectionally regulating the proliferation of human umbilical cord mesenchymal stem cells by non-gene modified light manipulation, and belongs to the technical field of mesenchymal stem cell culture applications. Background technique [0002] Many human diseases (such as liver disease, blood system disease, nervous system disease, etc.) are caused by the body's damage to cells, tissues, and organs from different reasons, resulting in abnormal, defective or lost cell functions. The current conventional medical treatment can only relieve symptoms, and it is difficult to cure the disease from the tissue level; while organ / tissue transplantation treatment faces serious problems such as the lack of organ sources and the high cost of postoperative anti-rejection treatment. Human umbilicalcord-derived mesenchymal stem cells (hUC-MSCs) refer to a kind of multifunctional stem cells that exist in neonatal umbilical cord tissue....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C12N13/00
CPCC12N5/0665C12N13/00
Inventor 李曦杨坤刘巧刘京郊
Owner SHANDONG UNIV
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