In-situ photocuring antibacterial bone defect repair gel and preparation method thereof

A light-curing, bacterial-bone technology, used in medical science, prosthesis, tissue regeneration, etc., to promote proliferation and osteogenic differentiation, and prevent infection

Active Publication Date: 2021-06-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above drawbacks have brought huge economic and ment

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  • In-situ photocuring antibacterial bone defect repair gel and preparation method thereof
  • In-situ photocuring antibacterial bone defect repair gel and preparation method thereof
  • In-situ photocuring antibacterial bone defect repair gel and preparation method thereof

Examples

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Embodiment 1

[0026] An in situ photocurable antibacterial bone defect repair gel, including copper sulfide nanoparticles (CuSNPs), 10% (w / w) GelMA photocurable hydrogel.

[0027] The preparation method of the above-mentioned bone defect repair gel comprises the following steps:

[0028] S1: Put copper acetate and chitosan (molecular weight 50,000-190,000Da, 75-85% deacetylation) in a three-necked flask, add 50mL dilute acetic acid solution, and stir vigorously for 24 hours under nitrogen protection;

[0029] S2: Weigh sodium sulfide, dissolve it in 1mL deionized water, add it dropwise to the flask, and continue stirring for 30 minutes;

[0030] S3: Connect the condenser, keep stirring, heat up to the crystal growth temperature at a rate of 5°C per minute, and keep warm for 30 minutes, then dialyze the solution in deionized water for 3-5 days to obtain CuSNPs;

[0031] S4: Take 100mL of CuSNPs (1.8mg / mL) and add it into a glass flask after sonication, and add 3mL of methacrylic anhydride d...

Embodiment 2

[0040] An in situ photocurable antibacterial bone defect repair gel, including methacrylylated modified CuSNPs, 5% (w / w) GelMA photocurable hydrogel.

[0041] The preparation method of the above-mentioned bone defect repair gel comprises the following steps:

[0042] S1: Put copper acetate and chitosan (molecular weight 50,000-190,000Da, 75-85% deacetylation) in a three-necked flask, add 50mL dilute acetic acid solution, and stir vigorously for 24 hours under nitrogen protection;

[0043] S2: Weigh sodium sulfide, dissolve it in 1mL deionized water, add it dropwise to the flask, and continue stirring for 30 minutes;

[0044] S3: Connect the condenser, keep stirring, heat up to the crystal growth temperature at a rate of 5°C per minute, and keep warm for 30 minutes, then dialyze the solution in deionized water for 3-5 days to obtain CuSNPs;

[0045] S4: Take 100mL of CuSNPs (1.8mg / mL) and add it into a glass flask after sonication, and add 3mL of methacrylic anhydride dropwise...

Embodiment 3

[0052] An in situ photocurable antibacterial bone defect repair gel, including methacrylylated modified CuSNPs, 7% (w / w) GelMA photocurable hydrogel.

[0053] The preparation method of the above-mentioned bone defect repair gel comprises the following steps:

[0054] S1: Put copper acetate and chitosan (molecular weight 50,000-190,000Da, 75-85% deacetylation) in a three-necked flask, add 50mL dilute acetic acid solution, and stir vigorously for 24 hours under nitrogen protection;

[0055] S2: Weigh sodium sulfide, dissolve it in 1mL deionized water, add it dropwise to the flask, and continue stirring for 30 minutes;

[0056] S3: Connect the condenser, keep stirring, heat up to the crystal growth temperature at a rate of 5°C per minute, and keep warm for 120 minutes, then dialyze the solution in deionized water for 3-5 days to obtain CuSNPs;

[0057] S4: Take 100mL of CuSNPs (1.5mg / mL) and add it into a glass flask after sonication, and add 3mL of methacrylic anhydride dropwis...

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Abstract

The invention provides in-situ photocuring antibacterial bone defect repair gel and a preparation method thereof. The gel is prepared by photo-crosslinking methylacryloyl copper sulfide nanoparticles and methylacryloyl gelatin. Modified CuSNPs is introduced into GelMA gel, and after the gel is added, CuSNPs-GelMA composite hydrogel can be obtained through in-situ photocuring shaping. Near-infrared light irradiates the composite material, the photothermal effect of CuSNPs in the CuSNPs-GelMA composite hydrogel can be activated, and mesenchymal stem cell proliferation and osteogenic differentiation of a periodontal defect area are promoted; and Cu< 2+ >slowly released by the composite material better endows a bone defect repair scaffold system with continuous antibacterial activity, and periodontal postoperative infection can be prevented to a great extent. The composite material is reasonable and convenient to prepare, low in price, high in application operability, good in biocompatibility and good in osteogenic effect, widens periodontal regeneration operation indications and is also suitable for repairing other bone defects.

Description

technical field [0001] The invention belongs to the technical field of bone repair, in particular to an in-situ light-cured antibacterial bone defect repair gel and a preparation method thereof. Background technique [0002] Periodontitis is the chronic infectious disease with the highest incidence rate. Periodontal pathogenic bacteria cause progressive irreversible alveolar bone resorption, eventually leading to tooth loss. Periodontitis has become the primary cause and main cause of adult tooth loss in my country and even in the world. How to promote alveolar bone repair and regeneration has always been a research hotspot of scholars. [0003] Periodontal regeneration framework is a hotspot in the research field of alveolar bone repair and regeneration. Existing scaffold materials are expensive, difficult to shape, difficult to construct and stabilize bone defect repair space, high technical sensitivity in application operation, and have no antibacterial properties and o...

Claims

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Application Information

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IPC IPC(8): A61L27/02A61L27/22A61L27/52A61L27/50A61L27/54
CPCA61L27/025A61L27/222A61L27/52A61L27/50A61L27/54A61L2430/02A61L2300/102A61L2300/404A61L2300/602
Inventor 孙伟莲杨涛李龑杨玉婷包佳琦张海正陈莉丽
Owner ZHEJIANG UNIV
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