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Application of nuezhenoside and specnuezhenide to facilitation of multiplication of in-vitro-cultured bone marrow-derived mesenchymal stem cell and inhibition of replicative senescence

A bone marrow mesenchymal and stem cell proliferation technology, applied in the field of medicine, to achieve the effects of inhibiting replicative aging, reducing immune rejection of the body, and promoting proliferation

Active Publication Date: 2018-11-16
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, the effects of Ligustrum lucidum and its components on stem cell proliferation and replicative senescence have not been reported.

Method used

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  • Application of nuezhenoside and specnuezhenide to facilitation of multiplication of in-vitro-cultured bone marrow-derived mesenchymal stem cell and inhibition of replicative senescence
  • Application of nuezhenoside and specnuezhenide to facilitation of multiplication of in-vitro-cultured bone marrow-derived mesenchymal stem cell and inhibition of replicative senescence
  • Application of nuezhenoside and specnuezhenide to facilitation of multiplication of in-vitro-cultured bone marrow-derived mesenchymal stem cell and inhibition of replicative senescence

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Embodiment Construction

[0026] The present invention will be further described below in conjunction with embodiment.

[0027] 1. Material method:

[0028] 1.1 Reagent DMEM low-sugar medium (Gibco BRL, U.S.); fetal bovine serum (FBS, Gibco BRL, U.S.); 0.25wt.% trypsin (containing 0.02wt.% EDTA, Gibco BRL, U.S.); penicillin-streptomycin DNA solution (Gibco BRL, USA); Cell Counting Kit-8 (Shanghai Beyond Biotechnology Co., Ltd., China); Cell Senescence β-Galactosidase Staining Kit (Shanghai Beyotime Biotechnology Co., Ltd., China);

[0029] 1.2 Instruments Electronic balance (AL204, Mettler Toledo, Switzerland); automatic microplate reader (ELX800, BioTekInstruments Inc., USA); carbon dioxide cell incubator (HF90, Health Force, China); biological safety cabinet (Type A2, Beijing East Lianhaer Instrument Manufacturing Co., Ltd., China); ultrapure water instrument (Milli-Q, Millipore Co., the United States);

[0030] 1.3 Isolation and culture of BMSCs 3-week-old male SD rats were killed by cervical disl...

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Abstract

The invention discloses application of nuezhenoside and specnuezhenide to facilitation of multiplication of an in-vitro-cultured bone marrow-derived mesenchymal stem cell and inhibition of replicativesenescence. The nuezhenoside and the specnuezhenide are coexistent, the nuezhenoside and the specnuezhenide have the effects of facilitating the multiplication of the in-vitro-cultured bone marrow-derived mesenchymal stem cell and inhibiting replicative senescence, and provide sufficient stem cells for clinical treatment, wherein the nuezhenoside and the specnuezhenide are commercially availablehigh-purity nuezhenoside and specnuezhenide (content is larger than or equal to 98 percent), and can be prepared according to a conventional method. The application disclosed by the invention has theadvantages that the nuezhenoside and the specnuezhenide can be used for facilitating the multiplication of the bone marrow-derived mesenchymal stem cell, and inhibiting the replicative senescence, sufficient cells are provided for tissue engineering and stem cell treatment of bone defect, cartilage defect, liver fibrosis, myocardial infarction, pulmonary fibrosis, diabetes and the like, and meanwhile, body immunological rejection reaction is reduced.

Description

Technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of privetin and privetin in promoting the proliferation of bone marrow mesenchymal stem cells cultured in vitro and inhibiting replicative senescence. Background technique [0002] Bone marrow mesenchymal stem cells, as a kind of cell-based medicine, have broad application prospects in diseases such as bone defect, cartilage defect, liver fibrosis, myocardial infarction, pulmonary fibrosis and diabetes. However, in the commercial production process, it is often difficult to expand the production scale of bone marrow mesenchymal stem cells. Among them, the slow proliferation of bone marrow mesenchymal stem cells, aging during the replication process, and the loss of stemness are the two main reasons that limit the expansion of its production scale and hinder the application and transformation of stem cells. [0003] Bone marrow mesenchymal stem cells ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0663C12N2501/999
Inventor 彭丽华许学寒欧阳宏伟
Owner ZHEJIANG UNIV
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