Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Traditional Chinese medicinal complex and preparation method thereof and human mesenchymal stem cell medium

A compound and culture medium technology, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve the problems of difficult to obtain bone marrow stem cells, inconvenient clinical application, slow expansion, etc., and achieve excellent antibacterial properties and preservation. performance, excellent cell proliferation, the effect of promoting cell proliferation

Inactive Publication Date: 2017-04-19
浙江译美生物科技有限公司
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology involves combining different ingredients with specific functions that work together synergistically towards enhancing their beneficial health benefits without compromising on any one aspect or reducing its negative impacts over time.

Problems solved by technology

This patents discuss various methods used by physicians during treatment planning or regimen management when treating diseases such as osteoporosis. However, there currently exist limitations associated with current therapies like calcium supplementations due to their poor biocompatibility properties. There also exists concerns about hormonal effects caused by estrogen use. These technical problem addressed by this patented study include finding new ways to improve the efficacy of existing drugs while reducing side effects related to them.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Traditional Chinese medicinal complex and preparation method thereof and human mesenchymal stem cell medium
  • Traditional Chinese medicinal complex and preparation method thereof and human mesenchymal stem cell medium
  • Traditional Chinese medicinal complex and preparation method thereof and human mesenchymal stem cell medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A preparation method for human mesenchymal stem cell culture medium, comprising the following preparation steps:

[0039] Step1: According to parts by weight, weigh 35 parts of donggui phthalide, 20 parts of methyl palmitate, 15 parts of cholestenyl myristate, 7 parts of methyl tanshinate, 5 parts of resveratrol, and 4 parts of succinate Acid, 4 parts of catechol, 8 parts of ginsenoside Rg1;

[0040] Step2: Fully mix the weighed components evenly to obtain the traditional Chinese medicine compound;

[0041] Step3: Add 1mg / L insulin, 0.05mg / L transferrin, 0.003mg / L sodium selenite and 2mg / L traditional Chinese medicine compound in sequence according to the mass volume ratio of DMEM / F12, and stir evenly. The traditional Chinese medicine compound is dissolved.

Embodiment 2

[0043] A preparation method for human mesenchymal stem cell culture medium, comprising the following preparation steps:

[0044] Step1: In parts by weight, weigh 40 parts of donggui phthalide, 18 parts of methyl palmitate, 14 parts of cholestenyl myristate, 5 parts of methyl salvianic acid, 4 parts of resveratrol, and 3 parts of succinate acid, 4 parts of catechol, 6 parts of ginsenoside Rg1;

[0045] Step2: Fully mix the weighed components evenly to obtain the traditional Chinese medicine compound;

[0046] Step3: Add 2mg / L insulin, 0.075mg / L transferrin, 0.004mg / L sodium selenite and 2mg / L traditional Chinese medicine compound in sequence according to the mass-volume ratio of DMEM / F12, and stir evenly. The traditional Chinese medicine compound is dissolved.

Embodiment 3

[0048] A preparation method for human mesenchymal stem cell culture medium, comprising the following preparation steps:

[0049] Step1: According to parts by weight, weigh 45 parts of donggui phthalide, 15 parts of methyl palmitate, 13 parts of cholestenyl myristate, 10 parts of methyl salvia, 3 parts of resveratrol, and 2 parts of succinate Acid, 5 parts of catechol, 7 parts of ginsenoside Rg1;

[0050] Step2: Fully mix the weighed components evenly to obtain the traditional Chinese medicine compound;

[0051] Step3: Add 3mg / L insulin, 0.1mg / L transferrin, 0.005mg / L sodium selenite and 3mg / L traditional Chinese medicine compound in sequence according to the mass volume ratio of DMEM / F12, stir evenly, The traditional Chinese medicine compound is dissolved.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a traditional Chinese medicinal complex and a preparation method thereof and a human mesenchymal stem cell medium. The traditional Chinese medicinal complex consists of the following components in parts by weight: 35-55 parts of ligustilide, 15-20 parts of methyl palmitate, 10-15 parts of cholesteryl myristate and 5-10 parts of methyl tanshinonate. The traditional Chinese medicinal complex provided by the invention has the beneficial effects that by combining the ligustilide, the methyl palmitate, the cholesteryl myristate and the methyl tanshinonate in use, and on the basis of mutual actions of the materials, the effect of the traditional Chinese medicinal complex provided by the invention on promoting the proliferation of human mesenchymal stem cells is enhanced.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Owner 浙江译美生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com