Traditional Chinese medicinal complex and preparation method thereof and human mesenchymal stem cell medium

A compound and culture medium technology, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve the problems of difficult to obtain bone marrow stem cells, inconvenient clinical application, slow expansion, etc., and achieve excellent antibacterial properties and preservation. performance, excellent cell proliferation, the effect of promoting cell proliferation

Inactive Publication Date: 2017-04-19
浙江译美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology involves combining different ingredients with specific functions that work together synergistically towards enhancing their beneficial health benefits without compromising on any one aspect or reducing its negative impacts over time.

Problems solved by technology

This patents discuss various methods used by physicians during treatment planning or regimen management when treating diseases such as osteoporosis. However, there currently exist limitations associated with current therapies like calcium supplementations due to their poor biocompatibility properties. There also exists concerns about hormonal effects caused by estrogen use. These technical problem addressed by this patented study include finding new ways to improve the efficacy of existing drugs while reducing side effects related to them.

Method used

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  • Traditional Chinese medicinal complex and preparation method thereof and human mesenchymal stem cell medium
  • Traditional Chinese medicinal complex and preparation method thereof and human mesenchymal stem cell medium
  • Traditional Chinese medicinal complex and preparation method thereof and human mesenchymal stem cell medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A preparation method for human mesenchymal stem cell culture medium, comprising the following preparation steps:

[0039] Step1: According to parts by weight, weigh 35 parts of donggui phthalide, 20 parts of methyl palmitate, 15 parts of cholestenyl myristate, 7 parts of methyl tanshinate, 5 parts of resveratrol, and 4 parts of succinate Acid, 4 parts of catechol, 8 parts of ginsenoside Rg1;

[0040] Step2: Fully mix the weighed components evenly to obtain the traditional Chinese medicine compound;

[0041] Step3: Add 1mg / L insulin, 0.05mg / L transferrin, 0.003mg / L sodium selenite and 2mg / L traditional Chinese medicine compound in sequence according to the mass volume ratio of DMEM / F12, and stir evenly. The traditional Chinese medicine compound is dissolved.

Embodiment 2

[0043] A preparation method for human mesenchymal stem cell culture medium, comprising the following preparation steps:

[0044] Step1: In parts by weight, weigh 40 parts of donggui phthalide, 18 parts of methyl palmitate, 14 parts of cholestenyl myristate, 5 parts of methyl salvianic acid, 4 parts of resveratrol, and 3 parts of succinate acid, 4 parts of catechol, 6 parts of ginsenoside Rg1;

[0045] Step2: Fully mix the weighed components evenly to obtain the traditional Chinese medicine compound;

[0046] Step3: Add 2mg / L insulin, 0.075mg / L transferrin, 0.004mg / L sodium selenite and 2mg / L traditional Chinese medicine compound in sequence according to the mass-volume ratio of DMEM / F12, and stir evenly. The traditional Chinese medicine compound is dissolved.

Embodiment 3

[0048] A preparation method for human mesenchymal stem cell culture medium, comprising the following preparation steps:

[0049] Step1: According to parts by weight, weigh 45 parts of donggui phthalide, 15 parts of methyl palmitate, 13 parts of cholestenyl myristate, 10 parts of methyl salvia, 3 parts of resveratrol, and 2 parts of succinate Acid, 5 parts of catechol, 7 parts of ginsenoside Rg1;

[0050] Step2: Fully mix the weighed components evenly to obtain the traditional Chinese medicine compound;

[0051] Step3: Add 3mg / L insulin, 0.1mg / L transferrin, 0.005mg / L sodium selenite and 3mg / L traditional Chinese medicine compound in sequence according to the mass volume ratio of DMEM / F12, stir evenly, The traditional Chinese medicine compound is dissolved.

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PUM

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Abstract

The invention discloses a traditional Chinese medicinal complex and a preparation method thereof and a human mesenchymal stem cell medium. The traditional Chinese medicinal complex consists of the following components in parts by weight: 35-55 parts of ligustilide, 15-20 parts of methyl palmitate, 10-15 parts of cholesteryl myristate and 5-10 parts of methyl tanshinonate. The traditional Chinese medicinal complex provided by the invention has the beneficial effects that by combining the ligustilide, the methyl palmitate, the cholesteryl myristate and the methyl tanshinonate in use, and on the basis of mutual actions of the materials, the effect of the traditional Chinese medicinal complex provided by the invention on promoting the proliferation of human mesenchymal stem cells is enhanced.

Description

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Claims

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Application Information

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Owner 浙江译美生物科技有限公司
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