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Application of rehmannioside C and salvianolic acid A in promoting proliferation of bone marrow mesenchymal stem cells cultured in vitro and inhibiting replicative senescence

A bone marrow mesenchymal and stem cell proliferation technology, applied in the field of medicine, can solve the unreported problems of stem cell proliferation and replicative aging, and achieve the effects of reducing the body's immune rejection, inhibiting replicative aging, and promoting proliferation

Active Publication Date: 2018-12-07
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, the effects of rehmanthin C and salvianolic acid A on stem cell proliferation and replicative senescence have not been reported.

Method used

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  • Application of rehmannioside C and salvianolic acid A in promoting proliferation of bone marrow mesenchymal stem cells cultured in vitro and inhibiting replicative senescence
  • Application of rehmannioside C and salvianolic acid A in promoting proliferation of bone marrow mesenchymal stem cells cultured in vitro and inhibiting replicative senescence
  • Application of rehmannioside C and salvianolic acid A in promoting proliferation of bone marrow mesenchymal stem cells cultured in vitro and inhibiting replicative senescence

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Embodiment Construction

[0033] The present invention will be further described below in conjunction with the examples.

[0034] 1. Material method:

[0035] 1.1 Reagent DMEM low-sugar medium (Gibco BRL, U.S.); fetal bovine serum (FBS, Gibco BRL, U.S.); 0.25wt.% trypsin (containing 0.02wt.% EDTA, Gibco BRL, U.S.); penicillin-streptomycin DNA solution (Gibco BRL, USA); Cell Counting Kit-8 (Shanghai Beyond Biotechnology Co., Ltd., China); Cell Senescence β-Galactosidase Staining Kit (Shanghai Beyotime Biotechnology Co., Ltd., China);

[0036] 1.2 Instrument Electronic balance (AL204, Mettler Toledo, Switzerland); automatic microplate reader (ELX800, BioTekInstruments Inc., USA); carbon dioxide cell incubator (HF90, Health Force, China); biological safety cabinet (Type A2, Beijing East Lianhaer Instrument Manufacturing Co., Ltd., China); ultrapure water meter (Milli-Q, Millipore Co., USA);

[0037]1.3 Isolation and culture of BMSCs 3-week-old male SD rats were killed by cervical dislocation, and steril...

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Abstract

The invention discloses an application of rehmannioside C and salvianolic acid A in promoting proliferation of bone marrow mesenchymal stem cells cultured in vitro and inhibiting replicative senescence. The rehmannioside C and the salvianolic acid A coexist. The rehmannioside C and the salvianolic acid A has the functions of promoting the proliferation of the bone marrow mesenchymal stem cells cultured in vitro and inhibiting the replicative senescence, and provide enough stem cells for clinical treatment. The rehmannioside C and the salvianolic acid A are commercially available high-purity rehmannioside C and the salvianolic acid A (with the content being greater than or equal to 98%) or can be prepared according to a conventional method. The advantages of the rehmannioside C and the salvianolic acid A are that the rehmannioside C and the salvianolic acid A can promote the proliferation of the bone marrow mesenchymal stem cells and inhibit the replicative senescence, provide sufficient cells for tissue engineering for treatment of bone defects, cartilage defects, liver fibrosis, myocardial infarction, pulmonary fibrosis, diabetes and the like and stem cell therapy, and reduce bodyimmune rejection.

Description

Technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the application of rehmanthin C and salvianolic acid A in promoting proliferation of bone marrow mesenchymal stem cells cultured in vitro and inhibiting replicative senescence. Background technique [0002] Bone marrow mesenchymal stem cells, as a kind of cell-based medicine, have broad application prospects in diseases such as bone defect, cartilage defect, liver fibrosis, myocardial infarction, pulmonary fibrosis and diabetes. However, in the commercial production process, it is often difficult to expand the production scale of bone marrow mesenchymal stem cells. Among them, the slow proliferation of bone marrow mesenchymal stem cells, aging during the replication process, resulting in the loss of stemness are the two main reasons that limit the expansion of its production scale and hinder the application and transformation of stem cells. [0003] Bone marrow mesen...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0663C12N2501/999
Inventor 彭丽华许学寒欧阳宏伟徐恩峤
Owner ZHEJIANG UNIV
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