Method for promoting proliferation of human mesenchymal stem cells
A technology for proliferation of bone marrow mesenchymal and stem cells, applied in the direction of bone/connective tissue cells, animal cells, vertebrate cells, etc.
Inactive Publication Date: 2018-12-07
淮安康宜莱干细胞生物科技有限公司
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[0006] There is no report on the promotion of the proliferation of bone marrow mesenchymal stem cells by the above compounds
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[0020] The following describes the substantive content of the present invention in detail in conjunction with the drawings and embodiments, but does not limit the protection scope of the present invention.
[0021] 1. Experimental materials
[0022] DMEM / F12 medium, low-sugar DMEM medium, and fetal bovine serum were purchased from Gibco, USA. α-cedrene, β-cedrene, γ-cedrene and isocedrene are self-made or purchased, and the purity is not less than 98%.
[0023] Cell counting kit (CCK-8) was purchased from Dojin Chemical Research Institute of Japan Co., Ltd.
[0024] 2. Experimental method
[0025] 1. Isolation and culture of human bone marrow mesenchymal stem cells
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The invention discloses a method for promoting proliferation of human mesenchymal stem cells. The human mesenchymal stem cell is a progenitor cell of osteoblast in bone marrow, research on the osteogenic differentiative capacity of the human mesenchymal stem cell is a hotspot in the orthopedic field, and the method for promoting the stem cell to differentiate toward osteogenesis is a main method for preventing and treating osteoporosis. Currently, application of the mesenchymal stem cell is difficult in massively acquiring mesenchymal stem cells. The method discovers that alpha-cedrene, beta-cedrene and isocedrone can be used for effectively promoting reproduction of human mesenchymal stem cells without influencing the multi-directional differentiation, can be used as additives to preparea culture medium for promoting reproduction of the human mesenchymal stem cells, and can be used for preparing medicines for promoting reproduction of the human mesenchymal stem cells.
Description
technical field [0001] The invention belongs to the biological field and relates to a method for promoting the proliferation of human bone marrow mesenchymal stem cells. Background technique [0002] Bone marrow mesenchymal stem cells are the progenitor cells of osteoblasts in the bone marrow. The research on their osteogenic differentiation ability has always been a hot spot in the field of orthopedics, and promoting their osteogenic differentiation is also the main method for preventing and treating osteoporosis. one. [0003] At present, one of the difficulties in the application of bone marrow mesenchymal stem cells is how to obtain a large number of bone marrow mesenchymal stem cells. [0004] α-cedrene, β-cedrene, γ-cedrene and isocedrene are compounds isolated from cedar, and their chemical structures are as follows. [0005] [0006] So far, there has been no report on the promotion of the proliferation of bone marrow mesenchymal stem cells by the above compound...
Claims
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Login to View More IPC IPC(8): C12N5/0775
CPCC12N5/0663C12N2500/30
Inventor 赵立士陈名星
Owner 淮安康宜莱干细胞生物科技有限公司