Human fat mesenchymal stem cell transportation fluid and preparation method thereof

Abstract

The invention discloses a human fat mesenchymal stem cell transportation fluid and a preparation method thereof. The transportation fluid is prepared from the following components in percentage by mass: 30-50 percent of fetal calf serum, 10-20 percent of DMSO, 5-10 percent of 0.2% heparin calcium solution and 20-40 percent of 0.2% trehalose solution. The human fat mesenchymal stem cells are storedby adopting the transportation fluid, the cell death speed can be slowed, and the proliferation and differentiation activity of the fat mesenchymal stem cells can be maintained.

Description

technical field [0001] The invention relates to the field of transport fluid, in particular to a human adipose-derived mesenchymal stem cell transport fluid and a preparation method thereof. Background technique [0002] With the development of medicine, in the process of clinical application and scientific research practice, people's understanding of the preservation of isolated organs, tissues and cells has been continuously improved, and the standards have been continuously improved. In the process of preservation and transportation of some organs or tissues to be transplanted, It also puts forward higher requirements for the medium of cell survival. It is necessary to ensure the viability of organs, tissues or cells, but also to retain their original biological activity to the greatest extent, and at the same time prevent them from being polluted by bacteria, etc., and preventing their death. The properties of various organs, tissues or cells are different, and their st...

AI Technical Summary

Problems solved by technology

Among them, the transportation cost of the non-cell culture state under the low temperature of liquid nitrogen is high, the composition of the cryopreservation solution is not clear, and the activity of the cells after cryopreservation will decrease, which will have a certain impact on subsequent experiments.

For the transportation of adherent cells, the number of cells transported each time is small and consumes too much medium. Generally, the air transport mode cannot be used, and the transport liquid leakage is prone to occur, resulting in cell death.