Microelement composition having activating function on stem cells and application thereof

A technology of trace elements and activation, applied in the direction of cell culture active agents, non-embryonic pluripotent stem cells, liver cells, etc., can solve the problem of not providing trace element compositions for hematopoietic stem cells

Active Publication Date: 2016-06-22
苏州博棠再生医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a trace element composition with an activation effect on stem cells and its application, aiming at solving the problem that the prior art has not provided a reasonable trace element composition to The problem of promoting the activation of hematopoietic stem cells

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  • Microelement composition having activating function on stem cells and application thereof
  • Microelement composition having activating function on stem cells and application thereof
  • Microelement composition having activating function on stem cells and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Promoting Effect of Trace Element Composition on Hematopoietic Stem Cell Proliferation

[0060] Trace element composition is in the present embodiment:

[0061] Iron 0.5mmol

[0062] Copper 2 μmol

[0063] Zinc 8 μmol

[0064] Cobalt 37μmol

[0065] Manganese 42μmol

[0066] Chromium 6 μmol

[0067] Selenium 7nmol

[0068] Iodine 42μmol

[0069] Nickel 5 μmol

[0070] Molybdenum 8 μmol

[0071] Vanadium 0.8μmol

[0072] Tin 3nmol

[0073] Silicon 17mmol

[0074] Strontium 42μmol

[0075] Boron 4.5 μmol

[0076] Rubidium 62μmol

[0077] Lithium 31μmol

[0078] Germanium 49nmol

[0079] Titanium 6mmol

[0080] Tungsten 118μmol

[0081] First, the human hematopoietic stem cells obtained by CD34 magnetic bead sorting were cultured in the hematopoietic stem cell serum-free medium produced by Life, and the cells were inoculated in the culture bag and placed at 37°C, 5% CO 2 For cultivation, the culture bag was gently shaken every 12 hours, and the culture m...

Embodiment 2

[0083] Promoting effect of trace element composition on the proliferation of mesenchymal stem cells

[0084] Trace element composition is in the present embodiment:

[0085] Iron 1mmol

[0086] Copper 3 μmol

[0087] Zinc 5 μmol

[0088] Cobalt 47μmol

[0089] Manganese 25μmol

[0090] Chromium 7.5μmol

[0091] Selenium 4.5nmol

[0092] Iodine 33μmol

[0093] Nickel 6 μmol

[0094] Molybdenum 6.3 μmol

[0095] Vanadium 4.2μmol

[0096] Tin 8nmol

[0097] Silicon 23mmol

[0098] Strontium 44μmol

[0099] Boron 3.8 μmol

[0100] Rubidium 56μmol

[0101] Lithium 34μmol

[0102] Germanium 73nmol

[0103] Titanium 4mmol

[0104] Tungsten 89μmol

[0105] The medium formula for cultivating mesenchymal stem cells of the present invention is: the above-mentioned trace element composition is added to DMEM / F12 basic medium, 10% fetal bovine serum (FBS), in order to compare the medium described in the present invention with no addition The effect of the microelement compo...

Embodiment 3

[0107] Trace element composition activates adipogenic ability of mesenchymal stem cells

[0108] Trace element composition is in the present embodiment:

[0109] Iron 1.6mmol

[0110] Copper 5.5μmol

[0111] Zinc 12.5 μmol

[0112] Cobalt 74μmol

[0113] Manganese 43μmol

[0114] Chromium 4 μmol

[0115] Selenium 5nmol

[0116] Iodine 31μmol

[0117] Nickel 3.6 μmol

[0118] Molybdenum 4.5 μmol

[0119] Vanadium 2.4μmol

[0120] Tin 7nmol

[0121] Silicon 22mmol

[0122] Strontium 32μmol

[0123] Boron 7.5 μmol

[0124] Rubidium 63μmol

[0125] Lithium 38μmol

[0126] Germanium 71nmol

[0127] Titanium 6mmol

[0128] Tungsten 170μmol

[0129] Mesenchymal stem cells can be differentiated into adipocytes under appropriate induction differentiation conditions in vitro. In order to detect the adipogenic ability of mesenchymal stem cells after culture, the present invention designs the experimental group as mesenchymal stem cells obtained under the culture conditi...

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Abstract

The invention discloses a microelement composition having activating function on stem cells and application thereof. The composition is prepared from 0.1-2 mmol of iron, 1-10 micromoles of copper, 1-20 micromoles of zinc, 5-100 micromoles of cobalt, 5-50 micromoles of manganese, 1-10 micromoles of chromium, 1-10 nmol of selenium, 0.1-50 micromoles of iodine, 0.1-10 micromoles of nickel, 0.1-10 micromoles of molybdenum, 0.1-5 micromoles of vanadium, 1-10 nmol of tin, 1-30 mmol of silicon, 5-50 micromoles of strontium, 0.1-10 micromoles of boron, 5-100 micromoles of rubidium, 1-50 micromoles of lithium, 10-100 nmol of germanium, 1-10 mmol of titanium and 1-300 micromoles of tungsten. The microelement composition having the activating function on the stem cells is provided and applied to activation culture of the stem cells. Experiment results find that the microelement composition has the functions of promoting proliferation of hematopoietic stem cells and mesenchymal stem cells in vitro and does not influence the differentiation potential of the mesenchymal stem cells, and besides microelements have the functions of promoting activation and proliferation of stem cells in mammal bodies like mice.

Description

technical field [0001] The invention relates to the field of application of trace elements, in particular to a composition of trace elements capable of activating stem cells and its application. Background technique [0002] Trace elements refer to some chemical elements that are very small in the body but are crucial to a series of biological processes such as the early development of the individual and the maintenance of acquired life. On the one hand, a living body begins to develop from a fertilized egg, and goes through the formation of embryonic stem cells to tissues and organs and the acquired maintenance of mature individuals. After the formation of mature individuals, the number of stem cells in the body is very small, only 1 / 10,000 in adult tissues The cells on the left and right are stem cells, and it is these very small number of stem cells that play a vital role in the maintenance of life. Except for nerve cells that are immortal, most adult cells have a lifespa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0789C12N5/0775C12N5/0735C12N5/074C12N5/0797
CPCC12N5/0606C12N5/0607C12N5/0623C12N5/0628C12N5/063C12N5/0647C12N5/0662C12N5/0672C12N5/0678C12N5/068C12N5/0687C12N5/0689C12N5/0692C12N5/0695C12N5/0696C12N2501/00
Inventor 刘小青
Owner 苏州博棠再生医学科技有限公司
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