Method for adipose-derived adult stem cell labeling by using exogenous green fluorescent protein (GFP)

A technology of green fluorescent protein and adult stem cells, which is applied in the fields of fluorescence/phosphorescence, biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of high price, low result, limited supply, etc. The effect of research cost reduction
CN100420936CActive Publication Date: 2008-09-24SICHUAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SICHUAN UNIV
Publication Date
2008-09-24

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Abstract

The invention relates to a method for using an extrinsic green luminescence protein to label fat body stem cell. The method uses adenovirus vectors infection extrinsic green luminescence protein to get into the fat body stem cell of small mouse to displace original green luminescence protein GM small mouse fat body stem cell to label. It manifests that the Balb / c small mouse fat body stem cell of adenovirus vectors infection extrinsic green luminescence protein and the original green luminescence protein GM small mouse fat body stem cell have the same growth, value adding and diversity from morphology, mRNA and protein expressing level.
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Description

1. Technical field:

[0001] The present invention relates to a new method of using exogenous green fluorescent protein (GFP) to mark adipose body stem cells, more specifically, relates to a method of using exogenous green fluorescent protein (GFP) to transfect it into Balb / c In mouse adipose-derived stem cells, a new method for cell labeling in place of endogenous green fluorescent protein transgenic mouse adipose-derived stem cells. 2. Background technology:

[0002] Stem cells can be divided into embryonic stem cells and adult stem cells according to their sources. The research on embryonic stem cells is not very optimistic due to the constraints of ethics, racial concepts and potential allogeneic rejection.

[0003] Adult stem cells exist in mature tissues and organs of humans and mammals, have self-renewal and certain pluripotent differentiation potential, and are intermediate between totipotent cells and mature cells. Under natural conditions, adult stem cells tend to ...

Claims

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