140 results about "Cell labeling" patented technology

Disclosed herein are methods and systems for classifying cell labels, for example identifying a signal cell label. In some embodiments, the method comprises: obtaining sequencing data of barcoded targets created using targets in cells barcoded using barcodes, wherein a barcode comprises a cell label and a molecular label. After ranking the cell labels, a minimum of a second derivative plot of a cumulative sum plot can be determined. Using the methods, a cell label can be classified as a signal cell label or a noise cell label based on the number of molecular labels with distinct sequences associated with the cell label and a cell label threshold.

The present invention relates to microfluidic devices and methods for detecting rare cells. The disclosed microfluidic devices and methods integrate and automate sample preparation, cell labeling, cell sorting and enrichment, and DNA / RNA analysis of sorted cells.

The present invention relates generally to microelectromechanical systems (MEMS) devices for the manipulation of cells or groups of cells, such as oocytes, embryos, and sperm. In particular, the present invention relates to Cell Labeling MEMS devices (2F), Microinjection MEMS devices, IntraCytoplasmic Sperm Injection (“ICSI”) MEMS devices, Zona Coring MEMS devices, Enucleation MEMS devices, Enucleation / Nuclear Transfer MEMS devices, and Cytoplasmic Transfer MEMS devices. The present invention also relates to kits containing the MEMS devices of the present invention.

The invention relates to a cervical cancer pathological cell classification system of a microscope pathological photograph. The system comprises a detection network model and a classification networkmodel. The detection network model is trained by using incompletely classified cell labeling data, and is used for detecting cells in the microscope photograph after the training is completed. The classification network model is trained according to the incomplete cell classification annotation data, and is used for classifying the detected cells and predicting the specific types of the cells after the training is completed. According to the system, the pathological cell detection / classification model can be obtained by training incompletely labeled samples.

The invention belongs to the technical field of a light-emitting nanometer material and provides a one-step rapid controllable preparation method of full-emission fluorescence carbon quantum dots so as to solve the problems that the existing fluorescence carbon dots are single in wavelength and uncontrollable in preparation and the fluorescence quantum efficiency of the carbon quantum dots is low.The method comprises the following steps: modifying any two of amino, carboxyl and hydroxyl on a benzene ring, mixing with absolute ethanol and dissolving ultrasonically and completely to obtain a mixed solution; transferring the mixed solution to a high-pressure reaction kettle and performing high-temperature reaction in a drying oven to obtain a claybank or black solution, namely a full-emission fluorescence carbon quantum dot solution; and flexibly adjusting the type and the proportion of a reaction precursor, the reaction temperature and the reaction time and performing one-step rapid control to obtain the full-emission fluorescence carbon quantum dots. The raw materials are wide in source and low in price, the production equipment is cheap and the universality is high. The prepared full-emission fluorescence carbon quantum dots have high solubility and dispersibility in an aqueous solution and an organic solvent, have high fluorescence quantum efficiency and can be applied to cell marking and imaging.

The invention belongs to the technical field of nanometer materials, and particularly relates to a carbon quantum dot and a green preparation method and application thereof. The preparation method includes: dissolving natural macromolecular carbohydrates or derivatives thereof in water, mixing with a dilute acid aqueous solution or a dilute alkali aqueous solution, heating with stirring to obtaina mixed solution; heating the obtained mixed solution in a sealed autoclave and preserving heat to obtain dispersion liquid; subjecting the obtained dispersion liquid to ultrasonic dispersion and centrifugal separation to obtain upper-level solution which is an operational carbon quantum dot solution. The preparation method of the carbon quantum dot is simple, low in equipment requirements and easy for mass production, no organic solvent is used in a whole process, no pollution is generated, and the prepared carbon quantum dot can be used for cell marking, optical imaging, medicine transporting, biosensing and the like.

The invention provides a fluorescent probe for intracellular protein labelling as well as a synthesis method and an application of the fluorescent probe. The provided probe is synthesized in simple steps and has good light stability. Compared with existing fluorescent probes for cell labeling, the probe can be specifically bound with SNAP labels in a complicated system and label any protein in cells. The probe can be applied to detection of copper ions in the cells, fluorescence gradually disappears with increase of the concentration of the copper ions, and the copper ion detection function isrealized. The probe realizes labeling of any protein and detection of the copper ions in the complicated environment and has very important application value in the biological and medical fields.

The invention provides a cell labeling system and method. The method comprises the following steps: acquiring a cell image; manually labeling position information of all cells in the series of cell images to form a training set of a cell detection model, and training the cell detection model by adopting the training set; taking a pre-training model based on a large number of image data sets as a cell classification model; carrying out cell labeling on the to-be-labeled cell image by utilizing the two models; results are corrected in a manual labeling mode, when the corrected data volume of thetwo models reaches a threshold value, fine adjustment training is started to be conducted on the two models, the trained models are adopted for detection, and the process is cycled. According to thecell labeling system, the labeling process and function are designed according to the labeling requirement of a training sample needed by cell detection, particularly, the labeling device and the labeling model training device are designed, model training is conducted on the basis of manually labeling a small number of cells, automatic labeling with high accuracy can be achieved, and the cell labeling efficiency is greatly improved.

The invention discloses a synthesis method of a spiropyrane small-molecule fluorescent probe with extreme acid / extreme alkaline switch response and an application of the spiropyrane small-molecule fluorescent probe. By virtue of the synthesis method, naphthalene nucleus spiropyrane with two carboxyl groups and a phenolic hydroxyl group is synthesized by carrying out modification on three loca of spiropyrane, wherein the naphthalene nucleus spiropyrane has the characteristic of reversible switch type response on pH values of extreme acid / extreme alkaline; when the pH value is smaller than 1.0, red fluorescence is represented; when the pH value is 1.0-12.0, fluorescence is represented; when the pH value is greater than 12.0, blue-green fluorescence is represented. The synthesized spiropyrane small-molecule fluorescent probe also has the characteristics of being high in optical, thermal and chemical stability and structural designability, and excellent in biocompatibility and cell labeling property; meanwhile, the synthesized spiropyrane small-molecule fluorescent probe is safe and low in low toxicity, and is capable of smoothly entering bacteria for bio-labeling, implementing the pH response of extreme acid / extreme alkaline as same as that of solutions in organisms; furthermore, the synthesized spiropyrane small-molecule fluorescent probe can be used as a multifunctional cell fluorescence labeling dye and can be applied to scientific research and gene diagnosis in the field of biological medicines.

Methods of obtaining cells internally labeled with perfluorocarbon nanoparticles suitable for magnetic resonance imaging and spectroscopy are disclosed. Also disclosed are methods for obtaining magnetic resonance imaging data from labeled under clinically relevant scan times and field strengths. Finally, the application further discloses methods of specifically detecting and distinguishing magnetic resonance imaging and spectroscopy data from two distinct sets of cells labeled with distinct types of perfluorocarbon nanoparticles.

The invention discloses a sample pretreatment method based on flow combination ICP-MS (Inductively Coupled Plasma Mass Spectrometry) single cell protein detection, and belongs to the technical field of sample pretreatment of flow cytometry. The sample pretreatment method comprises the following steps: (1) collecting and transporting a whole blood sample; (2) performing PBMC (Peripheral Blood Mononuclear Cell) cell separation; (3) performing cell active dyeing; (4) performing cell stimulation; (5) performing cell immobilization; (6) performing surface antibody dyeing; (7) performing intracellular phosphorylated protein dyeing; (8) performing single cell labeling, and the like. With the combination of a metal element labeled antibody with cell surface antigen, labeled cells are mixed with beads as internal reference for standardization, dead cells and living cells are distinguished in the pretreatment process, then the situation that the testing result analysis and description are affected by too many dead cells is avoided, single cells are distinguished from cell dimmers, cell trimers or even cell multimers, and flow combination ICP-MS single cell protein detection requirements can be very well met.

The invention discloses an electrostatic spinning patterning collection method and an electrostatic spinning apparatus. Based on a conventional electrospinning technical method, through a composite electrospinning material and changing of a receiving apparatus, a novel orderly controllable receiving apparatus and a control method are developed, and an orderly electrospinning fiber structure body with a needed pattern shape is prepared through preparing an organic / inorganic composite material electrostatic spinning liquid with a certain ratio and changing a carrying portion. The method and the apparatus are high in controllability, fibers are directionally consistent and orderly, the electrospinning collection is more flexible, the control method is simpler, and the receiving patterning effect is better. According to the invention, obtained electrospinning fiber films in any shapes can also be applied to such biomedical fields as magnetic medicament transportation, cell marking, biosensors and the like, and the application of electrospinning in industries especially bio-manufacturing is expanded.

Disclosed are methods and apparatuses for generating susceptibility-related contrast images, as induced, e.g., by marker material interventional devices used for passive MR-guided interventions, or by particles or cells loaded with marker materials used for molecular imaging, cell-tracking or cell-labeling. Near a local magnetic field perturber a positive contrast signal emanates from local gradient compensation to form, e.g., a balanced SSFP type of echo, whereas everywhere else echoes are shifted outside of the data acquisition window.

A method for measuring cytoplasm viscosity based on quantum dot three-dimensional tracing is disclosed. The method includes firstly introducing quantum dots into cells through a lipid Lipo-2000 transfection manner to achieve cell labeling; subjecting the single and recognizable quantum dots dispersed in the cells to three dimensional positioning and tracing by adopting an optical modulating mannerthrough a build quantum dot three-dimensional imaging system; further solving mean square displacement values MSDs which are motion parameters of the quantum dots by utilizing three-dimensional motion information; selecting quantum dots meeting free diffusion characteristics according to mathematical characteristics of the MSDs; finally solving diffusion coefficient D of the quantum dots in cytoplasm according to the MSD values for all the quantum dots meeting free diffusion characteristics; and further acquiring the cytoplasm viscosity according to a Stokes-Einstein equation. In a state thatcells survive, the cytoplasm viscosity can be accurately acquired by the method, visual three-dimensional motion information of molecules in cytoplasm can be obtained, and technical bases for researching intracellular material transportation, signal transmission, metabolism, differentiation, and the like are provided.

Disclosed are methods and apparatuses for generating susceptibility-related contrast images, as induced, e.g., by marker material interventional devices used for passive MR-guided interventions, or by particles or cells loaded with marker materials used for molecular imaging, cell-tracking or cell-labeling. Near a local magnetic field perturber a positive contrast signal emanates from local gradient compensation to form, e.g., a balanced SSFP type of echo, whereas everywhere else echoes are shifted outside of the data acquisition window.

The invention provides a method for preparing a BCNO light-emitting nanosheet. The method includes the following steps that firstly, boric acid and dicyandiamide are placed in an oven for heating pretreatment, heating temperature is 110-130 DEG C, heating time is 4-6 hours, and boric acid and dicyandiamide are naturally cooled to room temperature after being heated; secondly, boric acid and dicyandiamide subjected to heating pretreatment are immediately placed in a reactor, then ethylene glycol is added, solution temperature is raised after stirring to dry the solution by distillation, and a precursor of the BCNO nanosheet is obtained; thirdly, the precursor, prepared in the second step, of the BCNO nanosheet is sintered at the temperature of 600-700 DEG C, the temperature increase rate is 5 DEG C / min, sintering time is 4-6 hours, the precursor is naturally cooled to room temperature, and the BCNO light-emitting nanosheet is obtained. The BCNO light-emitting nanosheet synthesized through the method is free of toxin and pollution, the emitting range is a blue and green light wave band, and the BCNO light-emitting nanosheet can serve as fluorescein to be used in the fields such as cell labeling, cell imaging and biomedicine.

The invention belongs to the technical field of biochemical separation and analysis, and particularly relates to a micro-fluidic chip, a system and a method for sorting and enriching cells in cerebrospinal fluid. The chip can avoid pretreatment operations such as sample pretreatment and cell labeling and realize fast and efficient sorting and enriching functions for a small quantity of cells in the cerebrospinal fluid and further can realize in-situ counting and fast distinguishing of the small quantity of cells in the cerebrospinal fluid in combination with an in-situ microtechnique. The system integrates fast and efficient separation and enrichment of the small quantity of cells in the cerebrospinal fluid and in-situ microscopic observation, can realize sorting, enrichment, counting, observation and detection for the small quantity of cells in the cerebrospinal fluid, is high in functionality and high in efficiency, and can be used for clinical fast and efficient detection for cerebrospinal fluid cells.

The invention discloses a beta-caryophyllene derivative as well as a preparation method and application thereof. The structural general formula of the beta-caryophyllene derivative provided by the invention is shown by a formula I, wherein R is selected from any one of the following groups: a hydroxy group, an amino group, an ester group and an amide group. The inventor of the invention has verified through experiments that a reaction factor has good reactivity, stability, easily available raw materials and a certain reaction rate at the same time. The application of this reaction factor is proved in corresponding protein labeling and cell labeling experiments, which provides a new idea for the development of related biorthogonal reaction factors.

The present invention provides method for categorizing circulating tumor cells (CTCs) using various cellular markers and revealing or non-revealing assays which provide beneficial insights for clinical staging and therapy decision making in cancer patients.

The invention provides a recombinant plasmid for a method for marking a bifluorescence protein molecule and a method for marking a bifluorescence protein molecule cell. The recombinant plasmid comprises a segment A coding tag protein A, a segment B coding tag protein B and a promoter at the upper stream of a segment AC, wherein the 5' end or the 3' end of the segment A merges a sequence C coding protein C to be detected; the 5' end or the 3' end of the segment B merges a sequence D coding protein D to be detected; and segments A-C and B-D are connected by an IRES sequence E. The marking method comprises the steps of constructing the recombinant plasmid for the method for marking the bifluorescence protein molecule, cell transfection and fluorescence marking. The recombinant plasmid and the marking method can express two target genes in all in the same carrier, marks two kinds of different proteins in the same cell, has simple and rapid marking process and greatly saves the marking time than that of a traditional antibody fluorescence marking method.

The invention relates to an image processing technology, is applied to the field of smart medical treatment, and discloses an abnormal cell automatic labeling method. The method comprises: performingGaussian convolution smoothing processing on a cell pathology picture to obtain an image pyramid; performing detection fitting on the image pyramid through a Hough transform circle detection method toobtain a low-power fitting region of interest; mapping the low-power fitting region of interest to the acquired cell pathology picture to generate a fitting high-power image; segmenting a region of interest in the fitting high-power image to generate a segmented high-power image; labeling and cutting the segmented high-power image through an adaptive threshold segmentation algorithm to obtain anabnormal cell labeling set; and mapping the abnormal cell labeling set to the cell pathology picture to obtain an abnormal cell labeling picture. The accuracy of abnormal cell labeling is improved, and the calculation and storage pressure is reduced. In addition, the invention also relates to a blockchain technology, and the abnormal cell label set can be stored in a blockchain.

The invention relates to a method for using an extrinsic green luminescence protein to label fat body stem cell. The method uses adenovirus vectors infection extrinsic green luminescence protein to get into the fat body stem cell of small mouse to displace original green luminescence protein GM small mouse fat body stem cell to label. It manifests that the Balb / c small mouse fat body stem cell of adenovirus vectors infection extrinsic green luminescence protein and the original green luminescence protein GM small mouse fat body stem cell have the same growth, value adding and diversity from morphology, mRNA and protein expressing level.

The invention discloses a cell labeling method, system and device. The method comprises the steps of: displaying a digital pathological slice image, wherein the digital pathological slice image comprises a plurality of cells; receiving a frame selection instruction, wherein the frame selection instruction is used for determining a frame selection area on the digital pathological slice image, the frame selection area includes one or more cells to be labeled on the digital pathological slice image; generating a shape outline of each cell in the box selection area according to the box selection instruction; receives annotation information added to cells within any of the shape contours. The invention realizes the dynamic display of the data marking result, so as to enhance the technical effect of the user experience in the process of waiting for the data output by the user.

The invention discloses an aggregation-induced emission effect-based multifunctional fluorescent polymer which is controllable in particle size and convenient to modify as well as a preparation methodand an application of the aggregation-induced emission effect-based multifunctional fluorescent polymer. The fluorescent polymer contains a structural formula unit as shown in a formula (I) which isdescribed in the specification, n is equal to 10-10000, and R is a structural group with an aggregation-induced emission function. A precipitation polymerization reaction is carried out on an AIE polymerizable unit, maleic anhydride, styrene and an optional cross-linking agent in the presence of an initiator to obtain the fluorescent polymer. The fluorescent polymer can be applied to immunoregulation, cell labeling, material antibiosis and roughness detection.

The invention relates to a phosphorescent iridium complex, and a preparation method and an application thereof, belongs to the technical field of organic photoelectric functional materials, and concretely relates to a preparation method of phosphorescent iridium complex molecules, and an application of the phosphorescent iridium complex molecules in molecule oxygen detection, and cell marking and imaging. The above complex material is composed of a cyclomedtalating ligand, a metal center and a benzyl substituted anthracene auxiliary ligand, has a structural general formula shown in the specification, has the advantages of long luminescence life, mild reaction conditions and easy purification, and has very good application prospects in anoxybiotic detection, cell imaging and pathology diagnosis.

The invention relates to a phosphorescent ionic iridium complex and a preparation method and application thereof, belongs to the technical field of organic photoelectric functional materials, and particularly relates to a preparation method of phosphorescent ionic iridium complexes and their application in amino acid detection, cell labeling and imaging fields. The phosphorescent ionic iridium complexes comprise a cyclometalation ligand, a metal center and an aldehyde-group-containing phenanthroline auxiliary ligand, and have the structural formula as shown in the specification, the synthesis steps of the material are simple, conditions are mild, and the method has good application prospect in the amino acid detection, cell labeling and imaging.

Methods of ex vivo labeling of a biological material for in vivo imaging, methods of labeling a biological material in vivo, methods for preparing a labeling agent, and methods for in vivo imaging of a subject using a biological material labeled with a labeling agent are disclosed. In one non-limiting example, the biological material is selected from cells and the labeling agent is a 89Zr-Desferrioxamine-NCS labeling agent.

The invention provides a non-natural difunctional saccharide 9-AzSiaDAz shown as a formula I and / or a non-natural difunctional saccharide 9-AzSiaNAl shown as a formula II, and preparing methods and applications thereof. The non-natural difunctional saccharides 9-AzSiaDAz and the 9-AzSiaNAl can be adopted as probes and used for cell labeling. A single function group cannot achieve high-throughput and real-time capturing of interactions of saccharides and saccharide-binding proteins and cannot perform double-color imaging. Difunctional non-natural saccharide probes developed from the non-natural difunctional saccharides, which contain orthogonal reaction groups and photo-crosslinking groups, can crosslink glycoprotein in a covalent manner to the non-natural difunctional saccharides through UV irradiation without the need of modifying the protein and can purify the protein through affinity capture. The difunctional non-natural saccharide probes with the two different orthogonal reaction groups can perform double-color imaging by a two-step Click reaction.

The invention relates to the technical field of nanomaterial preparation. A preparation method of fluorescent carbon dots comprises the following steps: drying blue algae powder as a raw material at 80 DEG C for 40 h until the water content of the blue algae powder falls to be 20% or lower to obtain dried blue algae powder; screening the dried blue algae powder with a stainless steel screen with pore diameter of 60 mm to obtain screened blue algae powder; mixing the screened blue algae powder with purified water, and performing stirring to obtain a mixture; transferring the mixture to an autoclave filled with polytetrafluoroethylene, performing heating at 120-180 DEG C for 8-12 h, and cooling a reactant to room temperature after heating, and collecting the reactant; filtering the reactantwith a microporous membrane with pore diameter of 0.22 mu m, performing dialysis with a dialysis bag for 30-36 h, and finally treating the product with a vacuum freeze-drying method to obtain the solid blue algae source carbon dots. The preparation method has simple operation and high production efficiency, and the prepared carbon dots can be applied to cell labeling, biological imaging, fluorescent probes and the like.