A method for measuring cytoplasm viscosity based on quantum dot three-dimensional tracing
A quantum dot, cytoplasmic technology, applied in measurement devices, indirect flow characteristic measurement, flow characteristics, etc., can solve the problems of inappropriate real-time tracking of quantum dots, inability to intuitively understand the three-dimensional motion characteristics of molecules, etc., and achieve high real-time performance and positioning. The effect of precision, accurate cytoplasmic viscosity
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[0031] The present invention will be described in further detail below in conjunction with the accompanying drawings.
[0032] A method for obtaining cytoplasmic viscosity based on three-dimensional tracking of quantum dots, comprising the following steps:
[0033] (1) Quantum dots label cells
[0034] This method takes HepG2 cells labeled with CdTe quantum dots as an example, adopts the liposome Lipo-2000 transfection method, and obtains the following operation process after multiple experimental parameter adjustments and optimizations:
[0035] (1) When the fourth-generation HepG2 cells are 80-90% confluent, digest them with 2.5% trypsin-EDTA, resuspend the culture medium, and count the cells. According to the counting results, dilute the cell density to 1.0× 10 5 cells / mL, take 1 mL of the cell suspension and inoculate it on a confocal culture dish (NEST) with a diameter of 20 mm. After culturing for 10 h, the cells are completely adhered to the wall; DMEM medium, CO 2 ...
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