Non-natural difunctional saccharides, and preparing methods and applications thereof

A non-natural, dual-functional technology, applied in the field of chemical glycobiology, can solve problems such as the inability to separate and identify carbohydrate-binding proteins, and the inability to achieve high-throughput and real-time capture of carbohydrates and carbohydrate-binding proteins
CN104177451AActive Publication Date: 2014-12-03PEKING UNIV
1 Cites 6 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
PEKING UNIV
Publication Date
2014-12-03

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a non-natural difunctional saccharide 9-AzSiaDAz shown as a formula I and / or a non-natural difunctional saccharide 9-AzSiaNAl shown as a formula II, and preparing methods and applications thereof. The non-natural difunctional saccharides 9-AzSiaDAz and the 9-AzSiaNAl can be adopted as probes and used for cell labeling. A single function group cannot achieve high-throughput and real-time capturing of interactions of saccharides and saccharide-binding proteins and cannot perform double-color imaging. Difunctional non-natural saccharide probes developed from the non-natural difunctional saccharides, which contain orthogonal reaction groups and photo-crosslinking groups, can crosslink glycoprotein in a covalent manner to the non-natural difunctional saccharides through UV irradiation without the need of modifying the protein and can purify the protein through affinity capture. The difunctional non-natural saccharide probes with the two different orthogonal reaction groups can perform double-color imaging by a two-step Click reaction.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of chemical glycobiology, in particular to the synthesis of unnatural bifunctional sugar and its application in cell surface markers. Background technique

[0002] Sialic acid has important biological functions and plays an extremely important role in anti-inflammation, anti-tumor, anti-viral therapy, etc. However, due to the limitations of traditional biochemical methods and gene technology, people's research on sialic acid at the cellular level relatively lagging behind. In recent years, the strategy of introducing chemical probes through the biological metabolic pathway of sugar has been favored by scientists. It uses chemical biology methods as an entry point, through the design of unnatural groups, bio-orthogonal reactive groups can be introduced on the cell surface, and probe molecules can be introduced under living cell conditions through bio-orthogonal reactions, and then in living cells The mechanism of spe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More