64 results about "Photo crosslinking" patented technology

This invention discloses an AfBPP-based active molecular probe for thiostreptin, its preparation, and its application, belonging to the field of chemical biology. This invention introduces a bifunctional tag integrating a photocrosslinking group (bisacrididine) and a bioorthogonal reactive group (alkynyl group) into the structure of thiostreptin. While retaining the original biological activity of the parent compound, it endows the probe with highly efficient probe function, solving the technical problem of target loss during washing and purification of traditional non-covalent probes. After target labeling, the probe can specifically connect to reporter groups such as fluorescein or biotin through click-chemical reactions, achieving efficient enrichment of drug targets. Combined with mass spectrometry analysis, it enables global identification of potential targets in cells or complex biological samples at the omics level, such as chemical proteomics, providing a powerful molecular tool for in-depth revelation of the potential targets and pharmacological mechanisms of thiostreptin.

The application discloses medical use of gelated cells loaded with sulfonated chitosan in treatment of atherosclerosis, and belongs to the technical field of biological medicine. The gelated cells loaded with sulfonated chitosan take macrophages as host cells, and intracellular cross-linking forms a photocrosslinking hydrogel network composed of methacrylated hyaluronic acid and sulfonated chitosan. The gelated cells completely retain cell membrane structure and membrane surface protein expression, lose the proliferation ability, and have excellent blood lipid selective adsorption capacity and foam cell lipid metabolism regulation capacity. In-vivo and in-vitro experiments prove that the gelated cells can significantly reduce blood lipid levels of atherosclerosis model animals, reduce aortic plaque area, and have good biological safety, thereby providing a new strategy and candidate drug for prevention and treatment of atherosclerosis, and having a good clinical application prospect.

The application discloses a kind of magnetically responsive hydrogel optical fiber and its preparation and application, belong to medical biomaterial field.The optical fiber includes from inside to outside: polyethylene glycol diacrylate and acrylamide photocrosslinking light transmission core layer;Calcium alginate low refractive index skin layer;Doped nano-magnetic response layer of ferriferrous oxide;And polydopamine grafting polyvinylpyrrolidone lubricating layer.It is prepared by coaxial extrusion and light / calcium step-by-step crosslinking.The structure realizes the spatial isolation and anti-interference of light transmission and magnetic drive, has low background light loss and anti-bending stability, has biomechanics matching, ultra-low friction and magnetic field accurate active navigation ability, solves the pain point that extremely soft optical fiber is difficult to directional intervention.Suitable for deep tissue precise photothermal therapy, in vivo continuous optical sensing and minimally invasive diagnosis and treatment integration and other frontier medical fields.

This invention belongs to the field of photocrosslinking probe technology, specifically relating to a GUSB photocrosslinking probe, its preparation method, and its application. The GUSB photocrosslinking probe has the general chemical formula C0. 13 H 18 N2O6-R, wherein R is any one of the oxalic group elements or peptide bonds. The R group covalently connects the glucuronic acid recognition core and the photosensitive unit in the GUSB photocrosslinking probe, and the R group contains no more than 4 atoms, resulting in a small size and low steric hindrance. The GUSB photocrosslinking probe provided by this invention uses glucuronic acid as the recognition core, combined with a small-volume diazacyclopropane photosensitive unit and a terminal alkyne group, to achieve µM-level in-situ covalent capture of active GUSB in complex systems such as cells or tissues. The probe of this invention significantly improves positioning accuracy, sensitivity, data availability, quantification, and enrichment, while also possessing advantages such as modular synthesis, mild conditions, and strong system compatibility, effectively addressing the technical shortcomings of existing technologies that struggle to accurately obtain GUSB activity in situ under low-dose conditions.

This invention provides a bilayer programmed release hydrogel dressing based on PD-168077. The hydrogel dressing is formed by photocrosslinking a lower fast-release layer and an upper slow-release layer. The fast-release layer is formed by photocrosslinking and curing a first pregel solution containing a first concentration of methacrylamide hyaluronic acid, methacrylamide collagen, a photoinitiator, and PD-168077. The slow-release layer is formed by photocrosslinking and curing a second pregel solution containing a second concentration of methacrylamide hyaluronic acid, methacrylamide collagen, a photoinitiator, and PD-168077. This invention also provides a method for preparing the dressing and its application. This invention constructs a biomimetic dermal triple network structure through a photopolymerization strategy of "single formulation, dual concentration, dual curing," achieving programmed release of PD-168077 with a fast-then-slow release. This synergistic effect of the drug, including anti-inflammatory, antioxidant, and angiogenesis-promoting properties, along with matrix bioactivity, effectively promotes the healing of chronic diabetic wounds.

This invention discloses a method for culturing skin organoids for large-scale production and the skin organoids used for large-scale production, belonging to the fields of tissue engineering and biomanufacturing technology. The method involves laying a dermal matrix layer composed of orthogonal double-network hydrogels embedding fibroblasts in a bioreactor containing multiple independently operable culture modules. After inoculating keratinocytes, negative pressure is used to drive a uniform descent of the culture medium level to achieve gas-liquid interface culture. The first network of the orthogonal double-network hydrogel is formed by photocrosslinking of methacrylamide recombinant collagen, and the second network is formed by Michael addition of thiolated polysaccharides and multi-arm polyethylene glycol maleimide. During the culture process, the barrier maturity is assessed in real time by online monitoring of transepithelial resistance, and the negative pressure is controlled accordingly. A single batch can produce 3000 cm³ of skin organoids. 2 The above-mentioned qualified skin organoids have a transepithelial resistance coefficient of variation of less than 10%.

This invention provides a cell microenvironment stabilizer that captures hydrogen ions, its preparation method, and its application, belonging to the field of biomedical technology. The preparation method of this cell microenvironment stabilizer includes the following steps: (1) mineralizing CaCl2 with TGF-β1, catalase, and polyethylene glycol-polyglutamic acid block copolymer to obtain mineralized nanoparticles; (2) preparing hydrogel microspheres under freezing conditions using a microfluidic device, and then photocrosslinking the frozen hydrogel microspheres; (3) mixing the hydrogel microspheres with the mineralized nanoparticles to prepare the cell microenvironment stabilizer. This invention prepares a mineralized hydrogel microsphere, which, as a cell microenvironment stabilizer, can be used to block the NLRP3 cascade in chronic inflammation, capturing excess hydrogen ions through the mineralization layer to neutralize the acidic microenvironment. This micro / nano bioreactor based on hydrogel microspheres can effectively interfere with NLRP3 in the microenvironment of degenerated tissues from inside and outside the cells, inhibiting chronic inflammation.

A single-cell transcriptome-guided multimodal synergistic injectable magnetically responsive biomimetic hydrogel system, its preparation method, and its application are described. This hydrogel platform is a three-level composite system of "matrix-microsphere-nanoparticle": the primary structure is a photocrosslinked methacryloyl hyaluronic acid three-dimensional network matrix; the secondary structure is GelMA microspheres loaded with MTPT nanoparticles prepared by microfluidic control; and the tertiary structure consists of bevacizumab and magnetite nanoparticles dispersed in the HAMA matrix. The MTPT nanoparticles have a mesoporous silica core, which is sequentially coated with temozolomide, a polydopamine coating, and a T7 targeting peptide. Under the activation of an alternating magnetic field, Fe3O4 generates a magnetothermal effect, which not only achieves local hyperthermia but also accelerates the time-sequential release of bevacizumab and MTPT, synergistically exerting the effects of chemotherapy, anti-angiogenic therapy, and hyperthermia.

The application discloses a kind of high adhesion hydrogel for electroencephalogram test and its preparation method and application, belong to biomedical engineering technical field.The hydrogel includes the following components crosslinked product: anionic monomer, cationic monomer, monomer and mutual penetration agent;The anionic monomer is any one of malic acid, gluconic acid, citric acid and lactic acid, cationic monomer is betaine or acetyl L-carnitine, monomer is N, N-dimethyl acrylamide, acrylic acid, sodium acrylate, 4-acryloyl morpholine or sodium acrylamidomethyl propane sulfonate, mutual penetration agent is sodium chloride aqueous solution or sodium chloride water-retaining agent solution.Preparation, cationic monomer is mixed and dissolved, vacuum drying, adding photo initiator mixing, then mutual penetration agent is added, and it is obtained by ultraviolet light crosslinking.The hydrogel of the application has high adhesion (adhesion strength reaches 203.22 kPa), high ion mobility and excellent biocompatibility, and the signal gain in EEG and ECG test reaches 2-3 times of traditional conductive paste, which can be widely used in medical conductive paste field.

The present application provides a kind of composite hydrogel for scar repair and its preparation method, including photo-crosslinking hyaluronic acid methacrylate and layered silicate nanocrosslinking agent dispersed in photo-crosslinking hyaluronic acid methacrylate, wherein photo-crosslinking hyaluronic acid methacrylate is physically adsorbed with layered silicate nanocrosslinking agent to form a double crosslinking network structure.The present application can be firmly attached to the wound to prevent bacterial invasion, and can also release the long-acting, controllable slow release of load factor, extend the local treatment window, improve bioavailability, and through the double crosslinking structure, it can also simulate extracellular matrix, facilitate the transport of oxygen, nutrients and cell metabolic waste, greatly improve the scar repair effect.

This invention relates to the field of batteries, and proposes an in-situ photocrosslinked modified battery separator, its preparation method, and a battery thereof. An in-situ photocrosslinked modified battery separator comprises a separator prepared by an in-situ photocrosslinking modification method. The in-situ photocrosslinking modification method includes: sequentially mixing a polymer with additives, a first stretching treatment, an extraction treatment, impregnation with a photoinitiator solution, a second stretching treatment, heat treatment, and photocuring treatment. This technical solution solves key problems in related technologies, such as uneven photoinitiator penetration and incompatibility with production lines.

This invention discloses a magnesium alloy composite scaffold, its preparation method, and its application. The composite scaffold comprises a JDBM magnesium alloy scaffold with a highly interconnected porous structure and a composite hydrogel layer filling and encapsulating it. The hydrogel layer is formed by photocrosslinking and curing of methacrylamide-loaded glucose oxidase. This composite scaffold is a bio-battery-like therapeutic system. GOx catalyzes the biocatalytic oxidation of glucose to hydrogen peroxide, while magnesium alloy acts as an electroactive anode, together forming an adaptive electrochemical cascade reaction system. Through spontaneous oxidation dissolution and short-circuit electrochemical reactions, it rapidly removes hydrogen peroxide into water and oxygen while simultaneously promoting the oxidation of magnesium. 2+ Release. Therefore, this system not only transforms the harmful high-glucose microenvironment of diabetes into a microenvironment conducive to bone regeneration, but also achieves multiple synergistic effects of anti-inflammation, anti-oxidation, and bone promotion through the continuous supply of magnesium ions and the timely removal of H2O2, significantly promoting the repair and regeneration of bone defects under diabetic conditions.

The invention relates to a device comprising an expandable hollow hydrogel tube encapsulated by an ingestible capsule to enable the targeted delivery of large molecules such as biologics to the gastrointestinal (GI) mucosa. Upon ingestion, the capsule is arranged to release the hydrogel tube in the GI tract, where the hydrogel tube expands from a first configuration to a second configuration upon hydration. In its expanded state, the hydrogel tube exerts an outward radial force on the lining of the GI tract. In some embodiments, the outward-facing surface of the hydrogel tube comprises microneedles. The invention further relates to a hydrogel resin comprising: gelatin or poloxamer 407; a neutralisation agent; acrylic acid or sodium acrylate; a crosslinker; a photoinitiator; and a photoabsorber. The hydrogel tube of the invention may be produced by bioprinting and photo-crosslinking said hydrogel resin.

This invention provides a high-strength photopolymerization bio-3D printing method based on temperature-sensitive gelation assistance. The method includes: preparing a liquid bio-ink containing a temperature-sensitive photocrosslinking material and a photoinitiator; spreading the liquid bio-ink on a printing platform or the surface of a previously cured printing layer, and subjecting it to in-situ physical gelation at an environment below its physical gelation temperature to form a physical crosslinking network; while maintaining the physical gel state, patterning the bio-ink layer under light exposure to further form a chemical crosslinking network; repeating the spreading, physical gelation, and light exposure steps to achieve layer-by-layer stacking of a three-dimensional entity. This invention combines a low-temperature-induced physical gelation process with a photochemical crosslinking process to form a physical-chemical dual network structure, thereby improving the mechanical properties and forming stability of the printed entity.

This invention discloses a photocuring process visualization ink composition, comprising a photocrosslinkable precursor component, a photoinitiator component, and a free radical indicator. During photocuring, the free radical indicator reacts with free radicals generated by the photoinitiator under photoexcitation, changing from one color state to another. The light absorption peak of the free radical indicator is spectrally separated from the excitation wavelength of the photoinitiator. This invention also discloses a method for visualizing the photocuring process using the above composition and an additive manufacturing process with visualization monitoring capabilities. The method described in this invention is compatible with most photocuring systems based on free radical polymerization mechanisms, and the monitoring process is applicable to various photo-based 3D printing technologies such as volumetric additive manufacturing, digital light processing, and stereolithography, providing a material and methodological basis for process visualization and closed-loop control in bio-3D printing.

This invention relates to a quantum dot light-emitting diode (LED) based on functional layer interface photocrosslinking modification and its fabrication method, belonging to the field of optoelectronic materials and devices technology. The diode comprises sequentially stacked ITO glass substrate, hole injection layer, organic hole transport layer, quantum dot light-emitting layer, electron transport layer, and cathode. Its core technology lies in introducing an interface modification layer at at least one functional layer interface of the device, formed by photoinduced crosslinking of 4-(3-trifluoromethyl-3H-diazacyclopropene-3-yl)benzoic acid. Optionally, this modification layer further comprises a pentaerythritol tetra(3-mercaptopropionate) post-treatment layer. The interface modification can be located between ITO and the hole transport layer, between the quantum dot layer and the electron transport layer, or between the electron transport layer and the cathode. The fabrication method of this invention is simple, has good compatibility with solution processing technology, and can effectively suppress interfacial ion migration, passivate surface defects, and improve device stability.

The subject of the present invention is a hydrogel carrier, a pharmaceutical composition, use thereof and process for their preparation. The biopolymeric photocrosslinked delivery system for temozolomide obtained according to the invention should be particularly suitable for local therapy of brain glioblastoma.

The application discloses a curcumin photoaffinity probe molecule and a preparation method and application thereof, and the preparation method comprises the following steps: taking curcumin (compound II) as raw material, and reacting with 3-but-3-alkynyl-3-(2-iodoethyl) diazole alkene (compound III) under alkaline conditions to obtain the curcumin photoaffinity probe molecule (compound I), wherein a photo-crosslinking group and a biological orthogonal group are introduced at the 4th position of the curcumin photoaffinity probe molecule, the diazirine photo is the crosslinking group, can in-situ crosslink the adjacent binding protein under the condition of ultraviolet light, the alkynyl group is the biological orthogonal group, and the probe is coupled with fluorescein or biotin through a biological orthogonal coupling reaction, so that the probe is used for fluorescence detection on curcumin target protein in cells and identification of the curcumin target protein.

Methods, systems and adaptors for multiphoton imaging of, and laser-tissue interactions with, non-transparent tissue, such as ocular and periocular tissue, are provided. Aspects of the present invention include methods of imaging a structure through non-transparent tissue, such as ocular or periocular tissue, comprising: deploying an excitation source to transmit light energy to a structure through non-transparent tissue, such as ocular or periocular tissue, detecting light emitted from the structure via multiphoton excitation through the non-transparent tissue, such as ocular or periocular tissue, and imaging the structure based on the detected light. Aspects of the present invention further include methods of treating tissue, such as ocular or periocular tissue or dermatologic tissue, by manipulating an imaged structure, including, for example, providing non-incisional therapy, photo-tissue interactions, multi-photon-mediated damage, such as thermal damage, photo-disruption, photo cross-linking, blood vessel coagulation or ablating tissue. Aspects of the present invention further include methods of imaging dermatologic tissues or providing dermatologic treatments. Aspects of the present invention further include methods of providing cosmetic treatments. Aspects of the present invention further include methods of guiding delivery of gene therapy or cellular therapy in ocular or periocular tissue. Also provided are systems and adaptors for performing the methods described herein.

This invention relates to nanoparticle dispersants, methods for preparing the same, and aqueous dispersions of stable nanoparticles containing the dispersant. Specifically, the dispersant is suitable for dispersing nanoparticles and comprises an amphiphilic polymer having an amine-containing backbone and UV-photocrosslinkable functional groups covalently bonded to the backbone, and further comprising hydrophilic polyether segments and / or hydrophobic organic segments. The aqueous dispersion comprises a photocrosslinked nanoparticle dispersant and nanoparticles.

This invention discloses a microgel bioink suitable for volumetric bioprinting and its applications. The bioink is prepared by mixing a microgel, a photocrosslinking polymer solution, and a photoinitiator. The final concentration of the photocrosslinking polymer solution in the bioink ranges from 1.25% to 20%. All bioinks prepared can achieve crosslinking under green light and exhibit good printability. When applied to cell-loaded bioprinting, this bioink can be used to construct tissues or organs with channels and / or heterogeneous cell structures. Due to its contactless and 3D stereolithographic printing, it avoids the shear force damage to cells encountered in traditional extrusion printing and can complete the printing process within tens of seconds, thereby achieving high cell viability in bioprinted cells.

The present application relates to the technical field of agricultural sensor, and particularly relates to a PVA and HAMA composite hydrogel microneedle for crop monitoring and a preparation method thereof. The microneedle is composed of a microneedle layer formed by photo-crosslinking and a back layer formed by physical crosslinking. The preparation method comprises the following steps: preparing a photoinitiator solution; preparing and defoaming a HAMA solution; adding the HAMA solution into a PDMS microneedle mold, centrifugal filling, temperature control concentration and violet light curing to form the microneedle layer; then adding a PVA solution to the back of the microneedle and drying to obtain a microneedle array. The obtained microneedle has good mechanical strength, water absorption and biocompatibility, can penetrate the cuticle layer of plant leaves or stems under low damage conditions, and realizes the collection of trace liquid in the plant body. The microneedle can be used as a sensing substrate for monitoring pH, conductivity, moisture and ions, and can realize real-time non-invasive monitoring of the physiological state of crops. The present application has simple process, low cost and can be prepared on a large scale, and is suitable for the fields of smart agriculture, precision management and plant physiology research.

The present application relates to the technical field of high molecular hydrogel materials, and particularly relates to a fast photo-crosslinkable anti-swelling high-strength polyvinyl alcohol hydrogel and a preparation method thereof. The present application firstly performs alkyl modification on polyvinyl alcohol to enhance the hydrophobic bonding between polyvinyl alcohol chains and reduce the swelling rate of the hydrogel; then further introduces a photosensitive group to enable the polyvinyl alcohol to have fast photo-crosslinking capability; then forms a primary hydrogel with nanocrystalline crosslinking under the conditions of freezing and solvent phase transition; then forms a stable chemical crosslinking network through ultraviolet light irradiation; finally, the hydrogel is stretched and heat set in the stretched state to improve the polymer chain orientation degree and crystallization degree, so that a polyvinyl alcohol hydrogel material with high strength, low swelling, fast photo-curing and excellent biocompatibility is obtained. The obtained hydrogel can be widely applied in the fields of flexible electronics, biosensing, neural electrodes, tissue engineering and implantable medical devices.

This invention discloses a multifunctional Schiff base-photocrosslinked drug-loaded hydrogel. The hydrogel comprises Dendrobium officinale polysaccharide (ODop), gelatin-hydroxyphenylpropionic acid (Gtn-HPA), an amino-containing antibacterial drug, a photocrosslinking agent, and an oxidizing agent. Specifically, Dendrobium officinale polysaccharide (ODop) undergoes Schiff base crosslinking with the amino-containing antibacterial drug and gelatin-hydroxyphenylpropionic acid (Gtn-HPA). Gtn-HPA itself undergoes photocrosslinking to enhance mechanical strength. Under acidic pH conditions, the Schiff base crosslinking hydrolyzes, releasing the amino-containing antibacterial drug from the hydrogel. The hydrogel prepared by this invention exhibits excellent mechanical properties, controlled drug release capability, and superior bioactivity.

This invention discloses a method for preparing a hierarchical frustum-shaped porous synergistic light-trapping SERS substrate and its application, belonging to the field of surface-enhanced Raman spectroscopy and microfluidics. The preparation steps include: S1, self-assembly of monolayer PS microspheres onto a PI film; S2, formation of a frustum structure on the PI surface via ICP etching; S3, removal of residual PS microsphere template by toluene dissolution and formation of a micron-scale pit array using PDMS molding; S4, self-assembly of monolayer PS nanospheres within the micron-scale pits and covering the nanosphere array with SU-8 photoresist; S5, cross-linking of SU-8 with UV exposure and removal of the nanospheres to form nanopores; S6, electron beam evaporation of a gold film; S7, substrate characterization; S8, substrate functionalization; S9, fabrication of a microfluidic chip and sealing with the SERS substrate; S10, heavy metal ion trace detection. The hierarchical frustum-shaped porous synergistic light-trapping SERS substrate proposed in this invention exhibits excellent structural uniformity and chemical stability, good oxidation resistance, and high-density hotspots. Its micro-nano synergistic structure can effectively improve light energy utilization efficiency and form abundant surface plasmon resonance regions, thereby enhancing Raman scattering signals, which has important application value for trace detection of analytes.

The application discloses a kind of water-based super-hydrophobic coating and its preparation method and application, the preparation method includes steps: (1) the oil phase material with photo-crosslinking curing characteristics is mixed uniformly with photo-initiator, and oil phase is obtained;(2) nanoparticle and surfactant are added to aqueous solution and mixed uniformly, and water phase is obtained;(3) oil phase is mixed uniformly with water phase and ultrasonic dispersion, and oil-in-water Pickering emulsion is obtained;(4) oil-in-water Pickering emulsion is treated with ultraviolet lamp irradiation, and oil drop in emulsion is photo-cured to form stable water-based super-hydrophobic coating.The preparation method provided by the application is simple, and the water-based super-hydrophobic coating prepared has the advantages of green environmental protection, good stability and the like.

This invention discloses a method for preparing a ternary dynamic glycopeptide hydrogel bio-ink, comprising the following steps: dissolving methacrylamide gelatin, aldehyde-modified hyaluronic acid, hydrazide-modified gelatin, and a photoinitiator together in a solvent and mixing them evenly to obtain the bio-ink. This bio-ink constructs a dynamic covalent cross-linking network through reversible acylhydrazone bonds formed between aldehyde-modified hyaluronic acid and hydrazide-modified gelatin, providing a remodelable three-dimensional microenvironment for cells. Simultaneously, the photocrosslinking properties of methacrylic anhydride-modified gelatin are introduced to rapidly solidify and maintain overall shape stability during the printing stage, providing a remodelable cellular microenvironment during subsequent in vitro culture. This establishes an organoid culture platform with both good printability and excellent cell compatibility, providing a reliable material basis for subsequent drug screening and functional studies.

The present application relates to the technical field of gas detection, and particularly relates to a responsive aerogel and application thereof. The responsive aerogel is prepared according to the following steps: metal organic framework material is mixed and dissolved with lead acetate to obtain an adsorption and response integrated probe; silk fibroin, polyethylene glycol diacrylate and the adsorption and response integrated probe are mixed, and a light curing reaction is carried out under the action of a photo-crosslinking agent, and the responsive aerogel is obtained. The double-mesh aerogel base generated by the reaction of silk fibroin and polyethylene glycol diacrylate can adsorb water molecules in different humidity environments to cause the base to be wetted, and provide a saturated micro-water environment for the colorimetric reaction on the surface of the pore wall, thereby effectively reducing the interference of environmental humidity on the response. The quantitative method established by using the responsive aerogel can be used for high-sensitivity quantitative detection of hydrogen sulfide in livestock and poultry farms.