The invention discloses a method for preparing a gas separation membrane with high MOF filler content, and belongs to the technical field of membrane separation. Disperse MOF particles in a solvent to prepare MOF "ink", and then mix the "ink" with the polymer solution in a water bath and ultrasonically make the two compatible. The film solution obtained after the solvent evaporated was poured on a glass plate and scraped to remove the solvent to obtain a film with a high MOF filler content. Finally, the obtained membrane is immersed in an appropriate amount of polymer solution, and the solvent is completely volatilized to obtain a high-MOF filler gas separation membrane with high gas separation performance. The membrane can avoid the problem of agglomeration caused by excessive filler content in traditional mixed matrix membranes, which leads to the degradation of separation performance. Its MOF filler content can reach up to 70%, and it shows the 2 / N 2 , CO 2 / CH 4 、H 2 / N 2 、H 2 / CO 2 With moderate selectivity for such gas pairs, the gas permeability coefficient can reach thousands of barrers, which is expected to meet the requirements of practical industrial applications.