The application belongs to the technical field of
haematococcus pluvialis separation, and specifically discloses a ternary cross-linked
nanofiltration membrane, a preparation method and application. The
nanofiltration membrane comprises a polyethersulfone
ultrafiltration membrane base film, a polydopamine intermediate layer and a ternary cross-linked functional layer sequentially loaded on the surface of the polyethersulfone
ultrafiltration membrane base film. The preparation method comprises the following steps: preparing the polydopamine intermediate layer, anchoring oxidized
haematococcus pluvialis
polysaccharide, covalently cross-linking polyphenylated
chitosan, coordination cross-linking iron ions, and post-treatment to obtain the ternary cross-linked
nanofiltration membrane. The ternary cross-linked nanofiltration membrane is used for separating
haematococcus pluvialis
polysaccharide and
astaxanthin ester. The ternary cross-linked functional layer simultaneously has three separation abilities of size screening, charge regulation and coordination selection, realizes selective separation of
astaxanthin ester and neutral oil with similar molecular weights, and effectively retains macromolecular
polysaccharide.