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Photo-crosslinking sericin protein hydrogel and preparation method and application thereof

A technology of sericin and hydrogel, which is applied in the field of biomedical composite materials, can solve the problems of limited sources, degradation, unextracted sericin and pure sericin hydrogel, etc., and achieve good biodegradability and biocompatibility Effects of capacitive properties, good controlled drug release properties, and good autofluorescence properties

Active Publication Date: 2016-11-09
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the reported sericin hydrogels also have some corresponding defects. For example, the raw material of the sericin hydrogel extracted by low-temperature lithium bromide is a specific silk fibroin-deficient mutant cocoon, and the source is limited. The extracted sericin The protein can only be stored in a solution state at low temperature for a short period of time, and conventional sterilization will lead to its degradation or denaturation; while sericin extracted by high temperature alkaline water method can be sterilized and stored by simple filtration, but in the process of extraction The macromolecular protein is severely degraded and it is difficult to prepare pure sericin hydrogel, and it can only be mixed with other polymers to prepare hydrogel
At present, there is no report on the preparation of pure sericin hydrogel by extracting sericin with high temperature alkaline water method

Method used

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  • Photo-crosslinking sericin protein hydrogel and preparation method and application thereof
  • Photo-crosslinking sericin protein hydrogel and preparation method and application thereof
  • Photo-crosslinking sericin protein hydrogel and preparation method and application thereof

Examples

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Embodiment 1

[0097] Example 1 Preparation of sericin hydrogel provided by the present invention

[0098] Selection of cocoons:

[0099] Select silk fibroin-deficient cocoons (140 Nds, 185 Nds), silkworm cocoons (white jade, Haoyue), silkworm cocoons (A.mylitta), and commercial sericin powder (purchased by Huzhou Xintiansi Biotechnology Co., Ltd.), mainly The chemical composition is: sericin.

[0100] Step 1). Extraction of sericin

[0101] 1. Weigh 100 g of silk fibroin-deficient cocoons (140 Nds, 185 Nds) or silkworm cocoons (white jade, Haoyue) or A.mylitta, cut into pieces, and place them in a clean beaker. Wash with water 3 times, centrifuge at 3500 rpm for 5 minutes to remove water;

[0102] 2. Add 3~4 L of Na with a molar concentration of 0.02 mol / L to the cocoon fragments in step 1. 2 CO 3 Solution or 3~4 L of deionized water, put it in a constant temperature water bath 100 o C water bath for 1 hour to dissolve sericin to obtain sericin solution;

[0103] 3. Transfer the solution obtained in...

Embodiment 2

[0119] Example 2 Preparation of sericin hydrogel provided by the present invention

[0120] The preparation method of the sericin solution in this embodiment is the same as the preparation method of Example 1, except that step 2) is performed as follows:

[0121] 1. Weigh 6 g of sericin powder or commercial sericin powder in step 1) in 35 o Dissolved in 30mL phosphate buffer under C conditions;

[0122] 2. Add 0.024 g of methacrylic anhydride to 1) and react for 12-72 hours to obtain a methacryl-modified sericin solution;

[0123] 3. Transfer the methacryl-modified sericin solution to the pre-treated dialysis bag (molecular weight cut-off: 3.5 kDa), clamp it with the dialysis bag clamp, and place it in the bag containing ddH 2 In the O beaker, stir slowly for dialysis, and change the water every 3 hours for a total of 48 hours of dialysis;

[0124] 4. Lyophilize the methacryl-modified sericin solution in 3 and place it at -20 o C refrigerator keep for later use.

[0125] 5. Dissolve meth...

Embodiment 3

[0128] Example 3 Preparation of sericin hydrogel provided by the present invention

[0129] The preparation method of sericin solution in this embodiment is the same as the preparation method of Example 1, except that step 2) is performed as follows:

[0130] 1. Weigh 6 g of sericin powder or commercial sericin powder in step 1) in 35 o Dissolved in 30mL phosphate buffer under C conditions;

[0131] 2. Add 0.024 g of methacrylic anhydride to 1) and react for 12-72 hours to obtain a methacryl-modified sericin solution;

[0132] 3. Transfer the methacryl-modified sericin solution to the pre-treated dialysis bag (molecular weight cut-off: 3.5 kDa), clamp it with the dialysis bag clamp, and place it in the bag containing ddH 2 In the O beaker, stir slowly for dialysis, and change the water every 3 hours for a total of 48 hours of dialysis;

[0133] 4. Lyophilize the methacryl-modified sericin solution in 3 and place it at -20 o C refrigerator keep for later use.

[0134] 5. Dissolve methacry...

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Abstract

The invention relates to photo-crosslinking sericin protein hydrogel and a preparation method and application thereof. The preparation method comprises the steps that methyl propenyl modified sericin protein is prepared into a solution to be mixed with a photoinitiator (Irgacure 2959) solution, and the sericin protein hydrogel is obtained through ultraviolet irradiation. The obtained sericin protein hydrogel has the good biodegradability, autofluorescence characteristic and injectable property, and wounds caused by implanting or taking out biomaterials can be effectively reduced. Most importantly, the sericin protein hydrogel has good biocompatibility both in vivo and in vitro, and adhesion of carried cells can be effectively promoted. Serving as 2D and 3D cytoskeleton, the sericin protein hydrogel can effectively promote proliferation of the carried cells and promote the carried cells to form functional tissue under the condition of being free of serum support; serving as wound accessories, the sericin protein hydrogel can repair skin wounds and can serve as tissue engineering and regenerative medicine biological materials.

Description

Technical field [0001] The invention relates to the field of biomedical composite materials, in particular to a photo-crosslinked sericin hydrogel, and a preparation method and application thereof. Background technique [0002] Hydrogel is a polymer material with a three-dimensional network structure. Because of its high water content, softness, viscoelasticity and good biocompatibility, it has been used in medical transplantation, biosensing, and controlled drug release fields. In the field of tissue engineering, hydrogels can be used as 2D or 3D biological scaffolds to support cell growth and reproduction. [0003] The cross-linking methods of hydrogel usually include chemical cross-linking, high-energy radiation cross-linking, and ultraviolet light irradiation cross-linking. The main method used in the present invention is the ultraviolet light irradiation cross-linking method to prepare the hydrogel. The hydrogel prepared by this method has good biological properties, not o...

Claims

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Application Information

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IPC IPC(8): A61L27/52A61L27/60A61L27/22A61K47/42A61K38/02A61K49/00G01N21/35G01N21/64
CPCA61K38/02A61K47/42A61K49/0019A61K49/0073A61L27/227A61L27/52A61L27/60A61L2430/02A61L2430/06A61L2430/20A61L2430/22A61L2430/30A61L2430/32G01N21/35G01N21/6486G01N2021/3595C08L89/00
Inventor 王琳王征刘佳乞超徐鲁明李琪琳刘兴欣张剑常炳程
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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