Degradable silicon-based carrier material based on ZnO composite mesoporous silica as well as preparation method and application of degradable silicon-based carrier material

A technology of mesoporous silica and composite silica, which can be applied to medical preparations without active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, etc. Enhanced aggregation, enhanced targeting effect

Active Publication Date: 2022-02-11
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the poor degradability of existing silicon-based carrier materials or the problem that degradation products will cause toxic and side effects to the matrix, one of the purposes of the present invention is to provide a composite medium based on ZnO The degradable silicon-based carrier material of porous silica and its preparation method, in order to endow the silicon-based carrier material with good biodegradability, excellent targeting ability and drug controlled release performance, the second purpose of the present invention is to provide the carrier Application of materials as drug carriers

Method used

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  • Degradable silicon-based carrier material based on ZnO composite mesoporous silica as well as preparation method and application of degradable silicon-based carrier material
  • Degradable silicon-based carrier material based on ZnO composite mesoporous silica as well as preparation method and application of degradable silicon-based carrier material
  • Degradable silicon-based carrier material based on ZnO composite mesoporous silica as well as preparation method and application of degradable silicon-based carrier material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] In this example, a degradable silicon-based carrier material based on ZnO composite mesoporous silica was prepared.

[0067] The synthesis route of ZnO composite mesoporous silica nanoparticles (ZnO@MSN) is as follows: figure 1 As shown, the ZnO quantum dots were firstly modified on the surface, and then reacted with 3-aminopropyltriethoxysilane (APTES) to obtain aminated ZnO quantum dots (ZnO-NH 2 ), followed by the reaction of aminated ZnO quantum dots with [3-(methoxysilane) propyl] succinic anhydride to obtain ZnO quantum dot silane coupling agent (ZnOTMS). Using cetyltrimethylammonium bromide (CTAB) as a template, adding a homogeneous mixture of tetraethyl orthosilicate (TEOS) and ZnOTMS, hydrolyzed to obtain ZnO@MSN.

[0068] The synthetic route of degradable silicon-based carrier material (ZnO@MSN-SS-PEG-FA) based on ZnO composite mesoporous silica is as follows: figure 2 As shown, ZnO@MSN reacted with 3-mercaptopropyltrimethoxysilane (MPTS) to obtain mercapto-m...

Embodiment 2

[0090] In this example, the anticancer drug doxorubicin (DOX) was loaded on the ZnO@MSN-SS-PEG-FA prepared in Example 1, and the steps were as follows:

[0091] Add 10mg of ZnO@MSN-SS-PEG-FA into the DOX solution with a concentration of 2mg / mL, shake and stir at 50°C for 4h under dark conditions, so that DOX molecules enter the pores of ZnO@MSN-SS-pyr , solid-liquid separation, collecting particles, washing with pure water several times to remove DOX on the surface of ZnO@MSN-SS-pyr, and dispersing the obtained drug-loaded ZnO@MSN-SS-pyr in a PBS buffer solution with a pH value of 8.07 , add SH-PEG3400-FA, the mass ratio of ZnO@MSN-SS-pyr to SH-PEG3400-FA is 1:1, react at room temperature for 12h, separate solid and liquid, and wash with water to obtain drug-loaded nanoparticles DOX@ZnO@MSN -SS-PEG-FA.

[0092] Calculate the amount of free drug in the solution (recorded as free amount) by measuring the absorbance of the eluate at 481nm, and calculate the drug loading of DOX@Z...

Embodiment 3

[0096] In this example, performance tests were performed on the products prepared in each step in Example 1.

[0097] 1. Morphological analysis

[0098] Observation step ZnO-NH by transmission electron microscope (TEM) 2 microstructure, the results are as follows image 3 Shown, ZnO-NH 2 The average particle size of ZnO quantum dots is 3.6±0.3nm, and obvious lattice fringes are observed, and the fringe spacing is 0.26nm, which is consistent with the (001) crystal plane of ZnO quantum dots.

[0099] Observing the microstructure of ZnO@MSN by TEM, the results are as follows Figure 4 As shown, ZnO@MSN has a spherical or ellipsoidal structure with uniform size and good dispersion. The average particle size of ZnO@MSN is 102±2nm, and its pores are complete and show through-shaped pores.

[0100] In order to explore the distribution of elements in ZnO@MSN, Si, O, C, N and Zn elements were analyzed by scanning transmission microscopy and energy dispersive X-ray spectroscopy (STE...

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Abstract

The invention provides a degradable silicon-based carrier material based on ZnO composite mesoporous silica. The carrier material is ZnO composite mesoporous silica nanoparticles of which the surfaces are grafted through glutathione response type functional groups to block a valve, and the ZnO composite mesoporous silica nanoparticles are mesoporous silica internally loaded with ZnO quantum dots. The blocking valve is polyethylene glycol-folic acid, the blocking valve blocks the orifices of the ZnO composite mesoporous silica mesoporous silicon nanoparticles, the disulfide bond in the carrier material can be cut off by glutathione, after the disulfide bond is cut off, the blocking valve falls off to open the orifices of the ZnO composite mesoporous silica mesoporous silicon nanoparticles; meanwhile, the ZnO quantum dots in the carrier material can be dissolved in an acid environment to promote degradation of the carrier material. The silicon-based carrier material is endowed with good biodegradability, excellent targeting ability and drug controlled release performance. The invention also provides a preparation method of the carrier material and application of the carrier material as a drug carrier.

Description

technical field [0001] The invention belongs to the field of drug carrier materials, and relates to a degradable silicon-based carrier material based on ZnO composite mesoporous silica, a preparation method thereof, and an application of the carrier material as a drug carrier. Background technique [0002] Mesoporous silicon nanoparticles are considered as ideal drug carrier candidates due to their good biocompatibility, large specific surface area, suitable pore size and adjustable pore size, which can deliver therapeutic drugs to different cancer tissues. one. However, the biodegradability of silicon-based nanomaterials has attracted much attention, because the Si-O-Si framework in mesoporous silicon is very stable, and the degradation time of mesoporous silicon in the organism can be as long as 2 to 3 weeks. Mesoporous silicon will be absorbed by the reticuloendothelial system after being recognized by the human immune system, but because it is difficult to biodegrade, i...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K47/60A61K47/54A61K33/30A61K31/704A61P35/00
CPCA61K47/6923A61K47/6929A61K47/542A61K47/60A61K33/30A61K31/704A61P35/00A61K2300/00
Inventor 阮丽萍李瑞杨润乾宋潇
Owner SICHUAN UNIV
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