6 results about "Protein labeling" patented technology

The present application relates to the technical field of fluorescent dyes, in particular to an asymmetric pentamethine cyanine compound. The compound comprises a structure as shown in formula I. The compound provided by the present application has good water solubility, near-infrared absorption, high fluorescence quantum yield and good molar absorption coefficient, and can be used in nucleic acid labeling, DNA sequencing, cell imaging, protein labeling or specific recognition of amino acids.

This invention provides a method and apparatus for splitting single-cell mixed sample sequencing data, relating to the field of biotechnology. The splitting method includes: capturing and sequencing single-cell suspensions using a single-cell platform; then performing reference genome alignment, cell identification, and gene expression level quantification on the sequencing data using Cellranger; splitting the cell data identified in step a into two groups of cell data for different sexes based on SNP locus information from the 1000 Genomes Project; and distinguishing the two groups of cell data from male or female samples based on the proportion of sex-specific genes expressed in the two groups of cell data. This splitting method eliminates the need for additional experimental operations such as protein labeling and genome sequencing, and can provide accurate and reliable data splitting even when individual SNP information is unavailable.

The application belongs to the technical field of biological detection, and particularly relates to a probe compound for CoA detection, a fluorescent probe and application, comprising a modified rhodamine series fluorescent dye, a pantothenate kinase inhibitor, a HaloTag, a Nanoluc luciferase and a pantothenate kinase fusion protein; through protein labeling, a series of biosensors are obtained, coenzyme A concentration in living cells can be rapidly detected, and rapid screening of coenzyme A drugs is regulated; through rational design and modification of the chemical probe molecules, and engineering modification of the sensor proteins, the chemical probes can label the sensor proteins in living cells, and then the bioluminescent sensors working in living cells are obtained, the requirements of rapid and high-sensitivity detection of CoA can be met, and the drugs capable of regulating the change of coenzyme A concentration can be high-throughput screened.

This invention discloses a rapid quantitative detection method for Salmonella using LFS-SERS, belonging to the field of food safety technology. The method includes the following steps: S1, lysing the sample to obtain a crude DNA solution; S2, using the crude DNA solution as a template, performing Salmonella-specific LAMP amplification to obtain a biotin-labeled product; S3, mixing dCas9 protein, sgRNA, SERS-labeled gold nanoparticle-probe conjugate, the biotin-labeled product, and a loading buffer solution, incubating at room temperature for 1-5 minutes, performing a colorimetric reaction using a lateral flow test strip, and using a portable Raman spectrometer connected to a smart terminal to detect the T line in the LFS using SERS, thus achieving quantitative detection of Salmonella in the sample. This method is highly specific, sensitive, and stable; the entire detection process can be completed within 40 minutes, and it is low-cost and easy to operate.

The application relates to the field of chemical proteomics, in particular to a probe for labeling protein, and a structure formula of the probe is shown as formula I; the probe realizes protein labeling by labeling active amino acids, wherein the active amino acids are tyrosine, lysine, serine and threonine. The probe provides a new auxiliary tool for covalent small molecule drugs, protein covalent ligand screening, drug or active natural product target discovery and elucidation of protein-protein interaction. By using the probe and combining mass spectrometry analysis technology, high-throughput detection is realized, so that the analysis difficulty is reduced, and the experimental period is shortened; in addition, the probe is suitable for complex biological samples, and protein does not need to be purified, and the operation is simple.