22 results about "Protein labeling" patented technology

The disclosed systems and methods involve the semi-quantitative detection of disease-specific protein signatures in biological samples. The system may include a housing with a motor driven substrate holder on rails. The substrate holder is configured to be positioned at multiple locations along the guide rails. Imaging sensors capture images of portions of the substrate at these locations. The system detects markers on the substrate and determines the location of wells based on these markers. Based on the determined location of the wells, color values for each pixel in the wells can be measured. These values may be analyzed to detect disease-specific protein signatures in a variety of sample types.

The present application relates to the technical field of fluorescent dyes, in particular to an asymmetric pentamethine cyanine compound. The compound comprises a structure as shown in formula I. The compound provided by the present application has good water solubility, near-infrared absorption, high fluorescence quantum yield and good molar absorption coefficient, and can be used in nucleic acid labeling, DNA sequencing, cell imaging, protein labeling or specific recognition of amino acids.

This invention provides a method and apparatus for splitting single-cell mixed sample sequencing data, relating to the field of biotechnology. The splitting method includes: capturing and sequencing single-cell suspensions using a single-cell platform; then performing reference genome alignment, cell identification, and gene expression level quantification on the sequencing data using Cellranger; splitting the cell data identified in step a into two groups of cell data for different sexes based on SNP locus information from the 1000 Genomes Project; and distinguishing the two groups of cell data from male or female samples based on the proportion of sex-specific genes expressed in the two groups of cell data. This splitting method eliminates the need for additional experimental operations such as protein labeling and genome sequencing, and can provide accurate and reliable data splitting even when individual SNP information is unavailable.

The invention relates to a nitroimidazole group meso-substituted heptamethine cyanine dye as well as a preparation method and application thereof. According to the dye, a nitroimidazole group is introduced into the middle position of a heptamethine cyanine parent nucleus, so that the dye has excellent absorption characteristics in a near-infrared region, the excitation wavelength is 730-780 nm, the emission wavelength is 800-860 nm, and the dye has good tissue penetrating power. The nitroimidazole group can effectively quench fluorescence in an excited state through a light-induced electron transfer effect, energy is promoted to be released in a non-radiative transition mode, the photothermal conversion efficiency is remarkably improved, and meanwhile high light stability and low fluorescence quantum yield are kept. The dye can be further prepared into a nano preparation and shows excellent biocompatibility and tumor targeting ability. The compound has wide application prospects in the fields of non-diagnostic or therapeutic protein labeling, bioimaging, photothermal therapy and the like, is especially suitable for preparing efficient photosensitive reagent drugs for tumor photothermal therapy, and provides a new strategy for design of near-infrared photothermal diagnosis and treatment materials.

Provided are diagnostic and treatment methods,based on plasma protein markers for neurodegenerative diseases such as mild cognitive impairment (MCI) and Alzheimer's Disease (AD),as well as kits for diagnosing and / or treating these condition.Machine learning systems and methods for assessing the risk for a subject having a neurodegenerative disease based on measured protein marker levels are also provided.

The invention belongs to the technical field of biological medicines, and particularly relates to sulfonium salt polypeptide or protein as well as a preparation method and application thereof. Polypeptide or protein containing cysteine and a sulfonium salt compound are used as raw materials, and the sulfonium salt compound is used for alkylation of cysteine at a specific site of the polypeptide or protein to prepare the sulfonium salt polypeptide or protein. The sulfonium salt polypeptide or protein is used as a probe for covalently labeling tryptophan in a lysine methylation reader protein recognition pocket. According to the method disclosed by the invention, different types of sulfonium salt polypeptides and protein probes can be simply, conveniently and efficiently prepared, and the designed annular sulfonium salt with a novel skeleton has an obvious improvement on the polypeptide or protein labeling yield of reader protein. The prepared sulfonium salt probe not only can be used for identifying lysine methylation modified reader protein and finding a therapeutic target for disease-related pathways, but also can be used for modifying tryptophan at a specific site of an antibody-coupled drug and provides a powerful tool for targeted therapy research of tumors.

A protein marker panel for screening clinical futile recanalization (FR) in patients with large vessel occlusion-induced acute ischemic stroke (LVO-AIS) and use thereof are provided. The protein marker panel includes GUCA2A, CCL14, STAB1, and VSIG4. A combined diagnostic value of the protein marker panel is high, and receiver operating characteristic (ROC) curve analysis demonstrates an efficacy in distinguishing and screening FR patients, with an area under the curve (AUC) of 0.959, indicating desirable sensitivity and specificity. Such a protein marker panel shows promise as a significant indicator for clinical decision-making in FR evaluation.

The present invention relates to an integrated centrifugal device and method for analyte processing, particularly for the separation, enrichment, labeling, and analysis of nanovesicles such as extracellular vesicles (EVs). The device leverages a compact, rotatable substrate to execute multi-step assay protocols that may include chromatographic separation, centripetal fluid transport, immuno-capture, and protein labeling, all under low-speed centrifugal conditions. The device architecture enables automated, hands-free operation without requiring ultracentrifugation or specialized personnel. Fluidic modules including sedimentation, chromatography, displacement, and multiple assay chambers may be functionally integrated through a series of valves, channels, and immiscible liquid-based displacement mechanisms, facilitating precise sample handling, minimal loss, and multiplexed analysis.

Provided are a fluorescent probe, a preparation method therefor and a use thereof. The fluorescent probe sensitively and specifically responds to viscosity, and can be used for the specific fluorescence labeling of proteins as well as in the quantification, detection or kinetic study of proteins and the imaging of cells, tissues and living bodies.

The invention provides a vitamin B12 detection kit, belongs to the technical field of in-vitro diagnostic reagents, and particularly provides a vitamin B12 detection kit, which comprises: a labeling reagent, which comprises a vitamin B12 derivative labeled by a chemiluminescent marker; the alkaline dissociation agent comprises an alkaline solution, sodium chloride and urea; the acidic dissociation agent comprises an acidic solution, ethylenediamine tetraacetic acid, an organic solution and a reducing agent; and the solid-phase reagent comprises protein-labeled magnetic particles for specifically recognizing the vitamin B12. The vitamin B12 detection kit provided by the invention has relatively low detection limit and relatively high stability and accuracy, does not contain toxic reagents, and is suitable for clinical large-scale popularization and application.

The application discloses a transcription factor identification method based on a Transformer-Encoder and a multi-scale convolutional neural network. The method first extracts global features of a protein sequence using the Transformer-Encoder, then further extracts multi-scale local features from the global features using the multi-scale convolutional neural network, and finally fuses the extracted multiple features to output a probability of the protein sequence being a transcription factor. The transcription factor identification method based on the Transformer-Encoder and the multi-scale convolutional neural network can accurately identify whether an unknown protein sequence is a transcription factor, can quickly determine the transcription factor only by relying on the protein sequence, and greatly improves the protein labeling efficiency.

The invention discloses an adjacent protein marking method and application. The invention provides an adjacent protein labeling method, which comprises the following steps: by taking an aldehyde / keto-phenol small-molecule probe as a substrate, catalyzing the substrate into a substrate containing active free radicals by using a catalyst, and forming a covalent label by the substrate containing the active free radicals and a target protein to obtain a labeled protein. According to the method, the aldehyde / keto-phenol probe with high reaction activity is combined with a hydrazide / hydroxylamine reversible enrichment process, so that marker site identification with high sensitivity and high coverage is realized, and the limitations of site level analysis, cell physiological disturbance and the like of a traditional proximity labeling method are overcome. The method is simple to operate, good in reproducibility, high in data result reliability and accuracy and more real and reliable in identification result. By means of efficient site level analysis, the proteome can be identified and marked more accurately, and more sensitive protein structure analysis is realized.

The present invention relates to the technical fields of fluorescent dye synthesis, fluorescence imaging, and bio-sensing. Disclosed are an amide cyanine dye, an efficient synthesis method therefor, and a use thereof in fluorescence imaging. Compared with traditional cyanine dye synthesis methods, the amide cyanine dye synthesis method of the present invention is simple and has few by-products, short reaction time, high yield, a stable structure, and high universal applicability. By increasing the length of a conjugated chain, the absorption and emission wavelengths of the amide cyanine dye can be expanded from a short-wave near-infrared region to a near-infrared-II band. Experiments have proven that the amide cyanine dye has good photophysical properties and the potential for biofluorescence imaging, and can be used in biometric imaging, super-resolution fluorescence imaging of cellular mitochondria, protein labeling, specific recognition of antibodies, nucleic acid labeling, DNA sequencing, tumor photodynamic therapy, etc. On the basis of the advantages, the amide cyanine dye of the present invention has good prospects for application in the field of biofluorescence imaging research.

The invention relates to a caenorhabditis elegans model-based anti-aging effect evaluation method and application, and belongs to the technical field of anti-aging effect evaluation. The invention relates to a caenorhabditis elegans model-based anti-aging efficacy evaluation method, which comprises: S1, culturing a synchronized caenorhabditis elegans model on a nematode growth solid culture medium comprising a sample to be detected and E.coli OP50 escherichia coli for 3-9 days, and taking the cultured caenorhabditis elegans model as a sample group; culturing the synchronized caenorhabditis elegans model on a nematode growth solid culture medium containing E.coli OP50 escherichia coli for 3-9 days to obtain a blank group; s2, a plurality of caenorhabditis elegans models are selected from the sample group and the blank group for environment stress treatment; s3, performing fluorescence detection on the caenorhabditis elegans model subjected to environmental stress treatment, comparing the fluorescence intensity of a sample group with the fluorescence intensity of a blank group, and evaluating the anti-aging effect of the to-be-detected sample; wherein the caenorhabditis elegans model is a caenorhabditis elegans model of which SIR-2.1 protein is marked with fluorescent protein. And the anti-aging effect can be evaluated more accurately and efficiently.

The application belongs to the technical field of biological detection, and particularly relates to a probe compound for CoA detection, a fluorescent probe and application, comprising a modified rhodamine series fluorescent dye, a pantothenate kinase inhibitor, a HaloTag, a Nanoluc luciferase and a pantothenate kinase fusion protein; through protein labeling, a series of biosensors are obtained, coenzyme A concentration in living cells can be rapidly detected, and rapid screening of coenzyme A drugs is regulated; through rational design and modification of the chemical probe molecules, and engineering modification of the sensor proteins, the chemical probes can label the sensor proteins in living cells, and then the bioluminescent sensors working in living cells are obtained, the requirements of rapid and high-sensitivity detection of CoA can be met, and the drugs capable of regulating the change of coenzyme A concentration can be high-throughput screened.

Aspects herein relate, in part, to system and methods for evaluating a protein of interest. In particular, certain aspects use activity probes, which can be fused to recognition elements, to contact a protein of interest for further evaluation or analysis of the protein of interest.

This invention discloses a rapid quantitative detection method for Salmonella using LFS-SERS, belonging to the field of food safety technology. The method includes the following steps: S1, lysing the sample to obtain a crude DNA solution; S2, using the crude DNA solution as a template, performing Salmonella-specific LAMP amplification to obtain a biotin-labeled product; S3, mixing dCas9 protein, sgRNA, SERS-labeled gold nanoparticle-probe conjugate, the biotin-labeled product, and a loading buffer solution, incubating at room temperature for 1-5 minutes, performing a colorimetric reaction using a lateral flow test strip, and using a portable Raman spectrometer connected to a smart terminal to detect the T line in the LFS using SERS, thus achieving quantitative detection of Salmonella in the sample. This method is highly specific, sensitive, and stable; the entire detection process can be completed within 40 minutes, and it is low-cost and easy to operate.

The application relates to the field of chemical proteomics, in particular to a probe for labeling protein, and a structure formula of the probe is shown as formula I; the probe realizes protein labeling by labeling active amino acids, wherein the active amino acids are tyrosine, lysine, serine and threonine. The probe provides a new auxiliary tool for covalent small molecule drugs, protein covalent ligand screening, drug or active natural product target discovery and elucidation of protein-protein interaction. By using the probe and combining mass spectrometry analysis technology, high-throughput detection is realized, so that the analysis difficulty is reduced, and the experimental period is shortened; in addition, the probe is suitable for complex biological samples, and protein does not need to be purified, and the operation is simple.

The invention discloses a fluorescent dye targeting a human epidermal growth factor receptor 2 (HER2) as well as a preparation method and application of the fluorescent dye. The dye is composed of a fluorescent parent nucleus, a linker and a targeting group, and has near-infrared excitation and emission characteristics. The invention also provides an efficient preparation method of the dye. The dye has the innovativeness that fluorescence is quenched when the dye is in folded conformation in an aqueous solution, and the conformation is expanded and a strong fluorescence signal is released after the dye is specifically combined with HER2 protein, so that high signal-to-noise ratio detection is realized. The dye has the characteristics of quick response, high specificity, good biocompatibility and the like, and can be used in the fields of HER2 protein marking, molecular interaction research, drug screening, cell imaging and the like; and a novel fluorescence detection tool is provided for related research of HER2.

The invention discloses an amide cyanine dye as well as an efficient synthesis method and fluorescence imaging application thereof, and belongs to the technical fields of fluorescent dye synthesis, fluorescence imaging and biosensing. Compared with a traditional cyanine dye synthesis method, the amide cyanine dye synthesis method is simple, few in by-products, short in reaction time, high in yield, stable in structure and high in universality, the absorption and emission wavelength of the amide cyanine dye can be expanded from a short-wave near-infrared region to a near-infrared second-region wave band by increasing the length of a conjugated chain, and the absorption and emission wavelength of the amide cyanine dye can be expanded from a short-wave near-infrared region to a near-infrared second-region wave band. Experiments prove that the amide cyanine dye has good photophysical properties and biological fluorescence imaging potential, and can be applied to the aspects of biological recognition imaging, super-resolution fluorescence imaging of cellular mitochondria, protein labeling, specific recognition of antibodies, nucleic acid labeling, DNA sequencing, tumor photodynamic therapy and the like. Based on the advantages, the amide cyanine dye disclosed by the invention has a good application prospect in the field of biological fluorescence imaging research.

The present invention discloses superoxide-specific quinone methide precursors (QMP-SOs) that enable proximity labeling and chemoproteomics to investigate superoxide redox biology. QMP-SOs are activated by superoxide to generate a reactive quinone methide intermediate that covalently tags nearby proteins. These probes exhibit high selectivity toward superoxide over other ROS, enabling protein labeling in superoxide-rich cellular compartments. QMP-SOs are compatible with fluorescence imaging and tandem mass tag (TMT)-based mass spectrometry, facilitating visualization of superoxide dynamics and identification of superoxide-regulated proteins. In menadione-treated HepG2 cells, QMP-SO-TMT profiling identified mitochondrial proteins including DJ-1 and DLDH as redox-sensitive targets, linking superoxide stress to cell survival and metabolism. The QMP-SO platform uniquely combines spatial proximity labeling with superoxide specificity, allowing previously inaccessible insights into dynamic and localized redox signaling. This invention is broadly applicable in studying superoxide-mediated processes and holds potential for therapeutic target discovery in diseases driven by oxidative stress, including cancer, aging, and neurodegeneration.

The invention discloses an amantadine derivative as well as a preparation method and application thereof, and relates to the technical field of organic compounds. The amantadine derivative disclosed by the invention comprises a structure formed by connecting an amantadine group and an active group through PEG (Polyethylene Glycol), the active groups are selected from groups capable of reacting with amino acids. The series of amantadine derivatives provided by the invention can realize selective labeling of amino acid reactive groups of different types of proteins, can maximally reduce the influence of amantadine derivative labeling on the protein itself and the activity of the immobilized protein, and is suitable for the field of protein labeling. Amantadine group labeling is carried out on the protein through reaction with different types of groups on the labeled protein, and the method can be used for separation detection mediated by supermolecule macrocyclic host-guest interaction.