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Reagent for auxiliarily diagnosing lung cancer lymph node metastasis

A technology for lymph node metastasis and auxiliary diagnosis, applied in biological testing, material inspection products, measuring devices, etc., can solve the problems of low practicability and large clinical disconnection, and achieve increased usability, high reliability, and strong clinical use value Effect

Inactive Publication Date: 2012-05-09
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And its detection method has not yet been popularized in clinical diagnosis, and it is out of touch with clinical practice, and its practicability is not high

Method used

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  • Reagent for auxiliarily diagnosing lung cancer lymph node metastasis
  • Reagent for auxiliarily diagnosing lung cancer lymph node metastasis
  • Reagent for auxiliarily diagnosing lung cancer lymph node metastasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, the development process of reagent

[0019] The training set contains 255 cases of lung cancer. Firstly, a set of lung cancer tissue microarray was constructed, and the immunohistochemical staining and result analysis of a total of 23 candidate lung cancer-related marker proteins were completed using tissue chips containing lung squamous cell carcinoma. On this basis, the generalized linear regression model (Generalized Linear Model, GLM) was used to study the relationship between the expression status of the above 23 candidate marker proteins in 319 primary tumor tissues of lung squamous cell carcinoma and lymph node metastasis. The results showed that among the 23 proteins, the expression levels of 9 proteins were significantly correlated with lymph node metastasis (p<0.05). Among them, TIMP1, MMP1, TPX2, Aurora-A, uPA, Osteopontin, and Cathepsin-D were positively correlated with lymph node metastasis—the stronger their expression in the primary tumor, ...

Embodiment 2

[0023] Embodiment 2, the composition of reagent

[0024] The reagent consists of eight antibodies packaged individually; all eight antibodies are purchased, as follows:

[0025] Anti-MMP1 antibody: produced by Oncogene, the product catalog number is MS-802-P, and the concentration used is 1:300.

[0026] Anti-TIMP1 antibody: produced by Santa Cruz, the product catalog number is sc-21734, and the concentration used is 1:50.

[0027] Anti-IQGAP1 antibody: produced by BD Biosciences, the product catalog number is 610612, and the used concentration is 1:100.

[0028] Anti-CTSD antibody: produced by Santa Cruz, the product catalog number is sc-10725, and the concentration used is 1:100.

[0029] Anti-TPX2 antibody: produced by Biolegene, the product catalog number is 628002, and the used concentration is 1:500.

[0030] Anti-uPA antibody: produced by Calbiochem, the product catalog number is 1M13L, and the used concentration is 1:100.

[0031] Anti-pIgR / SC antibody: produced by...

Embodiment 3

[0036] Embodiment 3, the application of reagent

[0037] 52 patients with squamous cell carcinoma of the lung (volunteers who gave informed consent), 24 of them were patients with lymph node metastasis, and 28 were patients without lymph node metastasis. Carry out immunohistochemistry to 52 routine lung squamous cell carcinoma patients with the reagent of embodiment 2 (each patient carries out immunohistochemistry with eight kinds of antibodies in the reagent respectively, and the experimental material of immunohistochemistry is the primary tumor tissue of patient's surgical resection slices).

[0038] Each antigen was scored according to the immunohistochemical results. Immunohistochemical scoring standard for cytoplasmic antigen: qualitative score of cell staining intensity + quantitative score of cell staining area = scoring result; qualitative score of cell staining intensity is: no staining = 0, mild staining = 1, moderate staining = 2, strong staining Staining=3; the q...

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Abstract

The invention discloses a reagent for auxiliarily diagnosing lung cancer lymph node metastasis, comprising 8 antibodies which are used for detecting 8 protein markers which are MMP1 (matrix metalloproteinase-1), TIMP1 (tissue inhibitor of metalloproteinases metallopeptidase inhibitor 1), IQGAP1 (IQ motif containing GTPase activating protein 1), TPX2 (targeting protein for Xklp2), uPA (Urokinase-type plasminogen activator), Cathepsin-D, Fascin and pIgR / SC (polymeric immunoglobulin receptor / secretory component). By adopting the 8 antibodies and an immunohistochemical staining result, lung cancer lymph node metastasis can be auxiliarily diagnosed, and the reagent is expected to be used for the risk estimation of lung squamous cell cancer lymph node metastasis and the prognostic prediction. The reagent has high creditability, strong practicability and clinical use value based on the clinical routine immunohistochemical staining technology when the reagent is used for auxiliary diagnosis.

Description

technical field [0001] The invention relates to a reagent for auxiliary diagnosis of lymph node metastasis of lung cancer. Background technique [0002] Cancer has become one of the major diseases that seriously threaten human survival and health. Its morbidity and mortality are increasing year by year, and the number of new cases and deaths of lung cancer each year ranks among the top among various malignant tumors. Studies have shown that 90% of patients with squamous cell carcinoma of the lung died of tumor metastasis, which is partly due to the fact that lung cancer has no clear sentinel lymph nodes, and the operation is limited to the scope of lymph node dissection. There may be potential problems that cannot be detected by light microscopy. The tiny metastases of lymph node metastasis will eventually lead to the difficulty of diagnosis of lymph node metastasis, which will affect the formulation of treatment plan and the survival rate of patients. Therefore, the use of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574G01N33/577
Inventor 刘宇高燕宁林冬梅程书钧肖汀李琳谭金晶
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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