The application discloses a water ecological damage evaluation identification method based on high-
throughput environmental DNA sequencing, and belongs to the technical field of environmental damage investigation and identification, and comprises the following steps: collating a species
list of a target area, searching and downloading species sequence information, constructing a local
database, and supplementing the local
database information through specimen sequencing; collecting benthic animal samples of
multiple target area sample points, selecting test samples therefrom, extracting sample
DNA, amplifying and performing high-
throughput sequencing, performing species comparison according to the sequencing results, presetting multiple read number thresholds, judging whether species in a random mixed species sample and a quantitative proportion mixed species sample are detected, constructing an
environmental DNA sequencing and morphological mapping relationship model by using a
generalized linear model, optimizing the
environmental DNA sequencing and morphological mapping relationship model, calculating the
population resource quantity of a specific species in a natural sample, performing water ecological damage indicator species resource quantity evaluation and identification, and improving the water ecological damage identification accuracy and efficiency based on environmental
DNA.