Quantum dot microsphere immunochromatography test strip for detecting total amount of SARS-CoV-2 IgM-IgG antibody
A technology of quantum dots and test strips, applied in the field of quantum dot microsphere immunochromatographic test strips, can solve the problems of low detection rate of nucleic acid in throat swab samples, inability to meet on-site detection, low sensitivity, etc. The effect of meaning, high uniformity, and simple raw materials
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Embodiment 1
[0044] Example 1 Detects the Quantum Dot Microsphere Immunochromatography Test Strip for the Total Quantum Dot IgM-IgG Antibody to Novel Coronavirus
[0045] Such as figure 1As shown, a quantum dot microsphere immunochromatographic test strip for quantitatively detecting the total amount of novel coronavirus IgM-IgG antibodies, the test strip includes a bottom plate 7, a sample pad 1, a binding pad 2, a nitrocellulose membrane 5 and The absorbent pad 6, the sample pad 1, the binding pad 2, the nitrocellulose membrane 5 and the absorbent pad 6 are pasted end to end on the bottom plate 7 in sequence, overlapping each other by 2 mm, and the binding pad 2 is coated with the novel coronavirus N protein-quantum dot microspheres and S protein-quantum dot microspheres, the nitrocellulose membrane 5 is sequentially provided with a detection line 3 coated with a mixture of novel coronavirus N protein and S protein and coated with novel coronavirus N protein Quality control line 4 for a...
Embodiment 2
[0047] Example 2 Preparation of N protein and S protein (N protein-quantum dot microsphere and S protein-quantum dot microsphere) labeled with quantum dot microspheres
[0048] (1) Take quantum dot microspheres (Shanghai Kundao Biotechnology Co., Ltd., particle size 80-100nm) and sonicate for 1 minute, and adjust the concentration of microspheres with MES buffer (0.01M, pH 6.0) (final concentration is 0.5mg / mL), then add p-ethyl-N, N-dimethylpropylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS) (EDC and NHS powders are dissolved in MES buffer, EDC and The final concentration of NHS is 10mM), placed in a shaker at 37°C and incubated at a constant temperature for 0.5h to activate the carboxyl groups on the surface of the microspheres; then centrifuged in a centrifuge, discarded the supernatant, added MES buffer (0.01M, pH 6.0) to resuspend hang
[0049] (2) Divide the carboxyl-activated quantum dot microsphere buffer into 2 tubes, and add N protein (purchased from Guangdong ...
Embodiment 3
[0052] The preparation of embodiment 3 sample pads
[0053] Soak with buffer solution of 0.5% BSA (g / 100ml), 0.4% Tween-20 (v / v) for 30 minutes, dry for later use.
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