Quantum dot microsphere immunochromatography test strip for detecting total amount of SARS-CoV-2 IgM-IgG antibody

A technology of quantum dots and test strips, applied in the field of quantum dot microsphere immunochromatographic test strips, can solve the problems of low detection rate of nucleic acid in throat swab samples, inability to meet on-site detection, low sensitivity, etc. The effect of meaning, high uniformity, and simple raw materials

Pending Publication Date: 2020-07-17
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

RT-PCR needs to take samples from suspected patients (usually throat swab upper respiratory tract sampling), but the nucleic acid detection rate of throat swab samples is low, the sensitivity is not high, and it is easy to cause false negative results, and the operation of RT-PCR requires professional expertise Experimental personnel, expensive equipment and a safe

Method used

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  • Quantum dot microsphere immunochromatography test strip for detecting total amount of SARS-CoV-2 IgM-IgG antibody

Examples

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Effect test

Embodiment 1

[0044] Example 1 Detects the Quantum Dot Microsphere Immunochromatography Test Strip for the Total Quantum Dot IgM-IgG Antibody to Novel Coronavirus

[0045] Such as figure 1As shown, a quantum dot microsphere immunochromatographic test strip for quantitatively detecting the total amount of novel coronavirus IgM-IgG antibodies, the test strip includes a bottom plate 7, a sample pad 1, a binding pad 2, a nitrocellulose membrane 5 and The absorbent pad 6, the sample pad 1, the binding pad 2, the nitrocellulose membrane 5 and the absorbent pad 6 are pasted end to end on the bottom plate 7 in sequence, overlapping each other by 2 mm, and the binding pad 2 is coated with the novel coronavirus N protein-quantum dot microspheres and S protein-quantum dot microspheres, the nitrocellulose membrane 5 is sequentially provided with a detection line 3 coated with a mixture of novel coronavirus N protein and S protein and coated with novel coronavirus N protein Quality control line 4 for a...

Embodiment 2

[0047] Example 2 Preparation of N protein and S protein (N protein-quantum dot microsphere and S protein-quantum dot microsphere) labeled with quantum dot microspheres

[0048] (1) Take quantum dot microspheres (Shanghai Kundao Biotechnology Co., Ltd., particle size 80-100nm) and sonicate for 1 minute, and adjust the concentration of microspheres with MES buffer (0.01M, pH 6.0) (final concentration is 0.5mg / mL), then add p-ethyl-N, N-dimethylpropylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS) (EDC and NHS powders are dissolved in MES buffer, EDC and The final concentration of NHS is 10mM), placed in a shaker at 37°C and incubated at a constant temperature for 0.5h to activate the carboxyl groups on the surface of the microspheres; then centrifuged in a centrifuge, discarded the supernatant, added MES buffer (0.01M, pH 6.0) to resuspend hang

[0049] (2) Divide the carboxyl-activated quantum dot microsphere buffer into 2 tubes, and add N protein (purchased from Guangdong ...

Embodiment 3

[0052] The preparation of embodiment 3 sample pads

[0053] Soak with buffer solution of 0.5% BSA (g / 100ml), 0.4% Tween-20 (v / v) for 30 minutes, dry for later use.

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Abstract

The invention discloses a quantum dot microsphere immunochromatography test strip for detecting the total amount of a novel coronavirus IgM-IgG antibody. The test strip comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad, the combination pad, the nitrocellulose membrane and the water absorption pad are sequentiallyconnected end to end and adhered to the bottom plate, the combination pad is coated with an N protein-marker and an S protein-marker, and the nitrocellulose membrane is provided with a detection linecoated with an N protein and S protein mixture and a quality control line coated with an N protein antibody and S protein antibody mixture. The total amount of the novel coronavirus antibody is quantitatively detected by utilizing the test strip, the detection sensitivity is high, the accuracy is good, and the repeatability and the stability are good; the test strip is simple to operate and low in cost, can quickly and quantitatively detect the total amount level of the SARS-CoV-2 IgM-IgG antibody in a human body, and provides powerful support for epidemic situations.

Description

technical field [0001] The invention belongs to the production and technical field of medical diagnostic products, in particular to a quantum dot microsphere immunochromatographic test strip for detecting the total amount of SARS-CoV-2 IgM-IgG antibodies. Background technique [0002] In 2019, an acute respiratory infectious disease caused by an as-yet-undiscovered coronavirus infection emerged. The virus was named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by the World Health Organization (WHO), and the novel coronavirus pneumonia (COVID-19) caused by this virus has caused 80,303 people nationwide Infection, 2947 deaths (as of 2020.03.04). [0003] The new coronavirus belongs to the new coronavirus of the genus β. It has an envelope, and the particles are round or oval, often pleomorphic, with a diameter of 60-140nm. Through the comparison between the new coronavirus and SARS in 2003, that is, severe acute respiratory syndrome Whole-genome comparisons of...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/543
CPCG01N33/56983G01N33/558G01N33/54313
Inventor 刘斐王彬单衍可李宝杰李嘉豪
Owner NANJING AGRICULTURAL UNIVERSITY
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