Preparation method of goat mycoplasma pneumonia immune colloidal gold labeled rapid diagnosis test paper

Abstract

The invention discloses a preparation method of goat mycoplasma pneumonia immune colloidal gold labeled rapid diagnosis test paper, which comprises the following steps: preparing a goat mycoplasma pneumonia specific monoclonal antibody by using goat mycoplasma pneumonia BNCC126187 strain RPS11 gene plasmid recombinant purified antigen as an immune antigen; through colloidal gold particle monoclonal antibody protein labeling, purification, quality identification and condition optimization, establishing a mycoplasma pneumonia colloidal gold immunochromatographic assay method; and combining the obtained mouse RPS11 monoclonal antibody and a colloidal gold probe on a glass fiber membrane, and assembling with a solid-phase NC membrane to obtain the gold-labeled detection test strip. The gold-labeled diagnosis and detection test strip developed by the invention has good characteristics of rapidness, sensitivity, specificity and simplicity and convenience in operation on diagnosis and detection of mycoplasma pneumonia of sheep, and lays a good technical foundation for early diagnosis and detection of mycoplasma pneumonia of sheep and development of epidemiological investigation work.

Description

technical field [0001] The invention relates to the technical field of animal husbandry and veterinary medicine, in particular to a method for preparing a colloidal gold-labeled rapid diagnostic test paper for sheep mycoplasma pneumonia. Background technique [0002] Mycoplasma pneumonia of sheep and goats (MPSG) is caused by Mycoplasma ovipneumoniae (Movi), Mycoplasma mycoides subsp. Capri (Mmc) and Mycoplasma capricolum subsp. Capricolum , Mcc) and other mycoplasma are common contagious diseases in sheep and goats (Xu Chunguang et al., 2014). In late summer and autumn, sudden changes in temperature or long-distance transportation stress occur more frequently and become popular (Chen Huaitao, 2004). [0003] According to the serological investigation of Mycoplasma pneumoniae in non-immune sheep flocks in some areas of Guizhou by Guizhou Institute of Animal Husbandry and Veterinary Medicine (Wen et al., 2011), Mycoplasma pneumoniae has become a major infectious disease affe...

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Problems solved by technology

The disease is a conditional infectious disease with a long incubation period. When the clinical symptoms are obvious, it is often in the late stage of the disease, which brings difficulties to the treatment. Most of the sheep with clinical symptoms have a poor prognosis. In addition, the blind use of drugs in production leads to drug inhibition to the pathogen. Difficulty with separate diagnosis

[0004] At present, the professional large-scale breeding of mutton sheep in Guizhou is relatively lagging behind, and most areas are still dominated by small-scale scattered farming by farmers, mainly in remote mountainous areas. and experimental conditions, etc., are difficult to meet the technical needs of modern sheep production

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