Yellow fluorochrome and synthetic method and use thereof

Abstract

The invention discloses a yellow fluorescent dye, a method for synthesizing the same and application. The fluorescent dye has a structure shown as the general formula (I), wherein R1 and R2 are selected from chlorine, fluorine, bromine or hydrogen; and n is a positive integer between 3 and 7. The fluorescence emission spectrum of the yellow fluorescent dye is positioned in a yellow light region of visible spectrum; and the yellow fluorescent dye has high fluorescence quantum yield and good fluorescence labeling capacity, and can be used for protein labeling.

Description

technical field [0001] The invention belongs to the field of fluorescent dye synthesis, and in particular relates to a fluorescent dye with yellow light emission and a synthesis method and application thereof. Background technique [0002] With the rapid development of information technology, people have higher requirements for high-density and high-definition display technology. Fluorescence was discovered as early as the 16th century, but the principle and conditions of fluorescence were not clear until the middle of the 19th century. From 1867 when Goppetsroder used morin as a fluorescent reagent to detect aluminum by fluorescence method, until the beginning of the 20th century, people have known that more than 600 compounds including rhodamine, fluorescein, fused-ring aromatic hydrocarbons and eosin have fluorescence. Then Wawwillous measured the fluorescence yield (1924), and Gavila measured the fluorescence lifetime (1926). After the development of instruments combin...

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Problems solved by technology

[0004] How to obtain different kinds of labeling reagents is very difficult in the field of protein labeling

First, it is difficult to find fluorescent reagents that do not have overlapping emission wavelengths, because the half emission wavelength of typical organic fluorescent dyes is generally 40-80nm, while the visible w

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