Magnetic bead time resolution fluorescence immunoassay quantitative determination CK-MB (creatine kinase-MB) kit
A time-resolved fluorescence and immunoquantitative technology, applied in measurement devices, analytical materials, instruments, etc., can solve the problems of narrow quantitative measurement range of labeled enzymes and substrates, unsuitable for use in primary hospitals, poor sensitivity and accuracy, etc. Long fluorescence decay time, no radioactive pollution, and the effect of reducing background
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Examples
Embodiment 1
[0027] In this embodiment, the magnetic bead time-resolved fluorescence immunoquantitative detection kit for CK-MB consists of a reagent strip and a CK-MB calibrator.
[0028] Wherein, the shape of the reagent strip is fan-shaped, and 1 to 13 holes are arranged sequentially from left to right. Wells 1 and 2 are test wells for storing immunomagnetic beads and reagent wells; wells 3 and 4 are fluorescently labeled wells for storing europium-labeled antibodies; wells 6 and 7 are cleaning solution wells, It is used to store cleaning solution; No. 8 and No. 9 are sample diluent holes, which are used to store sample diluent; No. 12 is an enhancement fluid hole, used to store enhancement fluid; No. 5, 10, 11, and 13 are preparatory holes. A magnet can be stored between the 1st and 2nd wells for magnetic separation experiments. The 1st and 2nd wells can store a liquid volume of 300 μl; the 3rd and 4th wells can be disassembled from the entire reagent strip to become independent compon...
Embodiment 2
[0060] The making and detection of embodiment 2 reagent strips
[0061] The semi-finished reagent strips in this example are assembled through the following procedures: 50 μL CK-MB immunomagnetic beads, 200 μL europium-labeled CK-MB antibody, 2000 μL cleaning solution, 2000 μL Cleaning solution, 400 μL enhancement solution, and then seal with a film sealing machine. The sealing film is coated with product information that can be scanned and identified by an automatic fluorescence detection analyzer, including enterprise standard curve, batch, production date, and expiration date. The finished reagent strips, packaged CK-MB calibrator and other accessories are assembled into a kit.
[0062] Sample testing:
[0063] ①Add sample:
[0064] Put the sample or calibrator to be tested into the loading system of the automatic instrument, insert the reagent strip into the reagent strip slot, and the instrument will automatically identify the product information of the sealing film. A...
Embodiment 3
[0075] The comparison of embodiment 3 and commercially available kit
[0076] The commercially available creatine kinase assay kit (chemiluminescence microparticle immunoassay) (Abbott Trading Co., Ltd.) and the method in Example 1 were used to detect the sample (the concentration range of CK-MB in the sample was 1-300 ng / mL) Repeat three times to verify the correctness of the detection results of the kit of the present invention, the results are shown in Table 1 below.
[0077] Table 1 Sample test results
[0078]
[0079] Compared with commercially available reagents, the deviation of this reagent is less than ±5%, and the detection result of this reagent is accurate and reliable.
[0080] The commercially available creatine kinase assay kit (chemiluminescent microparticle immunoassay) (Abbott Trading Co., Ltd.) and the reagents in Example 1 were used to detect 200 clinical samples, and the test results are shown in Table 2 below.
[0081] Table 2 Test results of clinic...
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Abstract
Description
Claims
Application Information
- IPC
- G01N33/577
- CPC
- G01N33/577
- Inventors
- 李根平; 解巧丽