3 results about "Yellow fluorescent protein" patented technology

This invention belongs to the field of biosensor technology, specifically relating to a fusion protein, a 2-ketoglutaric acid biosensor, and its applications. Specifically, this invention combines the MifS extracellular sensing domain MifS-SD of the MifS / MifR two-component signal transduction system in Pseudomonas aeruginosa PAO1 with a superfolded circular rearranged yellow fluorescent protein, successfully constructing a 2-ketoglutaric acid biosensor with a high response amplitude. Experiments demonstrate that this biosensor can achieve real-time, in-situ detection of 2-ketoglutaric acid in microorganisms and mammalian cells, and can support the detection of related enzyme activities and the screening of inhibitors in a highly efficient, low-cost, and high-throughput manner, thus possessing good practical application value.

The invention belongs to the technical field of gene engineering, and particularly relates to an indole-3-acetic acid biosensor as well as a construction method and application thereof. The invention relates to an indole-3-acetic acid sensing plasmid. The plasmid carries a gene for coding a repressor protein, a promoter fragment specifically controlled by a transcription factor, a yellow fluorescent protein gene and a biosensor plasmid vector skeleton. The core of the invention lies in that the sensor has the advantages of high sensitivity, wide dynamic range and low background leakage, and can specifically respond to indole-3-acetic acid and various indole derivatives. The main application of the sensor is that the sensor is integrated into escherichia coli engineering bacteria for producing indole-3-acetic acid, so that high-yield strains can be quickly and efficiently screened from massive mutant libraries, and a key tool is provided for solving the industrialization bottleneck of indole-3-acetic acid microbiological method production.