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2 results about "Yellow fluorescent protein" patented technology
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Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm. Like the parent GFP, YFP is a useful tool in cell and molecular biology thanks to its properties useful for fluorescence microscopy.
A fusion protein, 2-ketoglutaric acid biosensor and its application
This invention belongs to the field of biosensor technology, specifically relating to a fusion protein, a 2-ketoglutaric acid biosensor, and its applications. Specifically, this invention combines the MifS extracellular sensing domain MifS-SD of the MifS / MifR two-component signal transduction system in Pseudomonas aeruginosa PAO1 with a superfolded circular rearranged yellow fluorescent protein, successfully constructing a 2-ketoglutaric acid biosensor with a high response amplitude. Experiments demonstrate that this biosensor can achieve real-time, in-situ detection of 2-ketoglutaric acid in microorganisms and mammalian cells, and can support the detection of related enzyme activities and the screening of inhibitors in a highly efficient, low-cost, and high-throughput manner, thus possessing good practical application value.
Owner:SHANDONG UNIV