218 results about "Yellow fluorescent protein" patented technology

Nucleic acid compositions encoding non-aggregating chromo/fluoroproteins and mutants thereof, as well as the encoded proteins, are provided. The proteins of interest are polypeptides that are non-aggregating colored and/or fluorescent proteins, where the non-aggregating feature arises from the modulation of residues in the N-terminus of the protein and the chromo and/or fluorescent feature arises from the interaction of two or more residues of the protein. Also provided are fragments of the subject nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.

This invention provides tandem fluorescent protein construct including a donor fluorescent protein moiety, an acceptor fluorescent protein moiety and a linker moiety that couples the donor and acceptor moieties. The donor and acceptor moieties exhibit fluorescence resonance energy transfer which is eliminated upon cleavage. The constructs are useful in enzymatic assays.

The disclosure provides proteins that can be used to determine the redox status of an environment (such as the environment within a cell or subcellular compartment). These proteins are green fluorescent protein (GFP) variants (also referred to as redox sensitive GFP (rosGFP) mutants), which have been engineering to have two cysteine amino acids near the chromophore and within disulfide bonding distance of each other. Also provided are nucleic acid molecules that encode rosGFPs, vectors containing such encoding molecules, and cells transformed therewith. The disclosure further provides methods of using the rosGFPs (and encoding molecules) to analyze the redox status of an environment, such as a cell, or a subcellular compartment within a cell. In certain embodiments, both redox status and pH are analyzed concurrently.

A system for optical tomography includes an apparent light source adapted to project excitation light toward a specimen having fluorescent proteins therein, wherein the excitation light enters the specimen becoming intrinsic light within the specimen, wherein the intrinsic light is adapted to excite fluorescent light from the fluorescent proteins, and wherein the intrinsic light and the fluorescent light are diffuse. A method of optical tomography includes generating the excitation light with the apparent light source, wherein the intrinsic light and the fluorescent light are diffuse.

The invention relates to a white light LED (light emitting diode) with high color rendering index, high light efficiency and low color temperature and a preparation method thereof. The white light LED comprises a support, an LED blue wafer and a fluorescent powder layer, wherein the LED blue wafer is fixed on the support; the fluorescent powder layer is coated on the LED blue wafer; and the fluorescent powder is a mixture of yellow fluorescent powder, red fluorescent powder, green fluorescent powder and an adhesive. The white light LED provided by the invention has the advantages of high color rendering index, high light efficiency and low color temperature.

The invention relates to a fluorescent agent color wheel and a light source system using the fluorescent agent color wheel. The fluorescent agent color wheel at least comprises glass, a first fluorescent agent and a first light filtering sheet. The glass is provided with a first segment. The first fluorescent agent coats the first segment. First wave band light is converted into second wave band light in terms of structure. The first light filtering sheet is arranged to be adjacent to the first segment and used for filtering part of first light beams of the first wave band light and the second wave band light so that a second light beam of the second wave band light emits out by penetrating through the first light filtering sheet. The second wave band light is yellow and the first fluorescent agent is a yellow fluorescent agent. The first filtering sheet is a red light filtering sheet. Thus, the manufacturing cost is effectively reduced, the luminance of red light is improved along with boosting of drive currents, therefore, the overall luminance of the light source system is improved and image quality is modified.

The invention is an LED device able to regulate color temperature, mainly additionally providing at least a red LED cell and at least a green LED cell outside a white LED device comprising blue LED cell and yellow fluorescent powder coating, to change white light color temperature, where the yellow fluorescent powder coating can only cover the blue LED cell, or additionally cover the red and green LED cells. Additionally, the blue, red and green LED cells can be separately driven to further improve color temperature.

The invention discloses a yellow fluorescent dye, a method for synthesizing the same and application. The fluorescent dye has a structure shown as the general formula (I), wherein R1 and R2 are selected from chlorine, fluorine, bromine or hydrogen; and n is a positive integer between 3 and 7. The fluorescence emission spectrum of the yellow fluorescent dye is positioned in a yellow light region of visible spectrum; and the yellow fluorescent dye has high fluorescence quantum yield and good fluorescence labeling capacity, and can be used for protein labeling.

The invention discloses a yellow fluorescent powder and a preparation method thereof and a light emitting device prepared from the fluorescent powder. The fluorescent powder has the chemical formula of A[x]Z[y]Ce[3-x-y]E[m]D[5-m]O[12], wherein A is one or more elements of La, Y, Lu and Tb, Z is one or more elements of Zn, Mg, Ca, Sr and Ba, E is one or more elements of Al, In and Ga, D is one or two of Si and Ge, 0<x<3, 0<y<=0.2, x+y<3, 4.8<=m<5, and y+m=5. The yellow fluorescent powder has the advantages of low production cost, simple process and higher light emitting intensity.

A composition includes an artificial construct having (a) a reporter-containing portion chemically coupled to (b) a cleavage site. The cleavage site interacts with an investigational substance in a manner that cleaves the reporter-containing portion from a remainder of the construct. The cleaved portion is destroyed or otherwise degraded by the local environment, and presence of an investigational substance is evidenced by reduction in signal from the reporter. The investigational substance is preferably a Botulinum toxin (BoTN), and the cleavage sequence is all or part of a SNARE protein. The cleavable reporter-containing portion is preferably Yellow Fluorescent Protein (YFP), Citrine, Venus, or a YPet protein.

The invention provides a transformation method utilizing a red fluorescent protein as a selection marker. The method comprises the following steps of: synthesizing a fluorescent protein (FP) gene and modifying according to the bias of a rice codon, wherein the modified FP gene is used as a selective marker gene of rice transformation and plant regeneration; driving the FP gene by a callus/seed coat-specific promoter, closely connecting with a target gene, and transferring to rice embryonic calli under the mediation of Agrobacterium tumefaciens; screening for three times by a fluorescence microscope in 30 days after coculture; and further verifying an obtained transgenic seedling by utilizing a polymerase chain reaction (PCR) and Southern hybridization. The result shows that the positive rate of the transgenic seedling is up to 80 percent, which proves that FP serving as the selection marker of plant transformation is completely feasible. By the method, the potential hazard of the traditional selection markers such as antibiotic and herbicide resistance genes and the like to environment and food safety is effectively reduced. The invention provides a novel safe plant genetic transformation screening system.

The invention discloses a fluorescent protein, a fusion protein, an isolated nucleic acid, a vector and application thereof, belonging to the technical field of biomedical optics and molecular imaging. The fluorescent protein disclosed in the invention further comprises the amino acid mutation site C158D compared with the conventional fluorescent protein mCrimson. The mutation site enables the fluorescence spectral properties of the fluorescent protein of the invention to be changed, and the fluorescent protein has substantial Stokes shift characteristics, is applicable to single-photon or multi-photon microscopic multicolor imaging, can effectively reduce crosstalk between the fluorescence spectra of different fluorescent proteins, enhances the sensitivity of imaging, ensures simultaneousfluorescent imaging of the fluorescent proteins with different spectra and reduces high requirements on optical devices.

Provided are four new fluorescent proteins. The proteins were derived from two wild-type fluorescent proteins: a red fluorescent protein (RFP) that was isolated from Actinodiscus or Discosoma sp. 1 and a green fluorescent protein (GFP) isolated from Montastraea cavernosa. Two mutant forms were generated from each wild-type protein. Each of the mutated forms has a higher fluorescence intensity than the respective wild-type form. The mutant forms of the fluorescent proteins allow for more sensitive detection of the fluorescence emitted by the proteins. Additionally, one of the mutant proteins is more resistant to photobleaching than its wild-type protein. The invention also encompasses isolated nucleic acids encoding the mutant forms of the wild-type RFP and GFP.

The biomedicine PTD4-GFP-Apoptin fusion protein for treating tumor has its synthesized PTD4 utilized to introduce Apoptin protein into cell, and its Apoptin further utilized to induce tumor apoptosis, so as to inhibit tumor growth. At the same time, green fluorescent protein (GFP) is utilized for observing the intracellular distribution of the fusion protein and showing the aggregation of the medicine in the target position, realizing the effective monitoring and timely regulation of the treating process. Experiment shows that the fusion protein can penetrate biomembrane to kill tumor cell effectively while generating no damage to health cell. The present invention is applied in treating tumor.

The invention discloses a light color-variable LED structure. The light color-variable LED structure is composed of blue light LED chips and a yellow fluorescent powder film, wherein the plurality of blue light LED chips are fixedly arranged on a COB substrate so as to form an LED array, and the yellow fluorescent film coats the LED chips, the yellow fluorescent powder film is of a periodic structure, and portions of the yellow fluorescent powder film which are corresponding to the different blue light LED chips have different thicknesses. The blue LED chips are alternately divided into an A group and a B group; the thickness or density of fluorescent powder on the yellow fluorescent powder film which is corresponding to the A group is small; the thickness or density of fluorescent powder on the yellow fluorescent powder film which is corresponding to the A group is large; color temperature corresponding to light emitted by the A group is high; and color temperature corresponding to light emitted by the B group is low; the A group and the B group are independently driven, so that the luminous intensity ratios of the A group and the B group are different, and therefore, the overall light color of the LED changes, thus, adjustability of light color of the LED can be realized through controlling the driving ratios of the A group and the B group.

The present invention relates to novel fluorescent protein variants of DsRed and eqFP578. Fluorescent protein variants having increased photostability and/or having reversible photoswitching behavior, as well as polynucleotides encoding such variants are provided herein. Methods of using these novel fluorescent protein variants and methods for constructing other fluorescent protein variants having increased photostability are also provided by the present invention.